Tumour budding and other prognostic pathological features at invasive margins in serrated colorectal adenocarcinoma: a comparative study with conventional carcinoma

Abstract

Compared to CC, SAC displayed more HG-TB, HG-CyPs and fewer PLI at the invasive margins and this may account for its poorer clinical outcome. TB, CyPs, TGP and PLI are useful histological prognostic aids in SAC.

Immunohistochemical expression profile of β-catenin, E-cadherin, P-cadherin, laminin-5γ2 chain, and SMAD4 in colorectal serrated adenocarcinoma

“…We have previously reported a different histological phenotype in SAC [16] and have confirmed the higher incidence of TB in this tumor type. Here we have studied the immunophenotype shown in the CT and at the IF, particularly in TB and non-TB aggregates, in a wellcharacterized series of SACs.…”

“…Population, salient clinicopathological features, and follow-up information of patients with CRC included in this study have been previously reported [15,16]. The histological criteria for the diagnosis of SAC include epithelial serrations, clear or eosinophilic cytoplasm, abundant cytoplasm, vesicular nuclei, absence of or less than 10% necrosis of the total surface area, mucin production, and cell balls and papillary rods in mucinous areas of a tumor [17].…”

“…None of the CRC cases (both SACs and CCs) in this study displayed all the histological features proposed for high-grade microsatellite instability (MSI-H) carcinoma diagnosis [18]. TB was assessed as described previously [16]. Briefly, an isolated single cancer cell or a cluster of fewer than 5 cancer cells was defined as budding foci.…”

“…We have previously shown that SAC has a worse outcome than conventional carcinomas (CCs) [15]; and more recently, we have proposed that its greater aggressiveness may be due to the presence of a particular histological phenotype shown at the IF, namely, high-grade (HG)-TBs, HG-cytoplasmic pseudofragments (HG-CyPs), infiltrative tumor growth pattern, and weaker lymphocytic response [16].…”

Targeting the serrated pathway of colorectal cancer with mutation in BRAF

“…This process resembling epithelial-mesenchymal transition does usually not occur in serrated carcinomas of the CIMP-H/BRAF/MSI-H type. By contrast, in other carcinomas from the serrated pathway such as those showing CIMP-H/BRAF/MSS [7,14,89,90] tumor budding is frequently observed.…”