Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma

Essa, Ahmed; Yamazaki, Manabu; Maruyama, Satoshi; Abé, Tatsuya; Babkair, Hamzah; Raghib, Adel M; Megahed, Eman; Cheng, Jun; Saku, Takashi

doi:10.1016/j.pathol.2016.02.006

Cited by 16 publications

(26 citation statements)

References 38 publications

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“…In a study involving 30 cases of well‐differentiated oral SCC, Essa et al observed that MGCs are CD68 + /CD204 + /CD163 − . Recently, the same immunoprofile was reported for MGCs induced from monocytes in a direct coculture with ZK‐1 cells (derived from human tongue SCC) . According to Essa et al , the absence of CD163 expression in MGCs indicates their partial differentiation into the M2 phenotype.…”

Section: Discussionmentioning

confidence: 54%

“…Secretion of cytokines by the tumor microenvironment seems to influence tumor progression and may also determine the formation of MGCs . These cells have been identified in several epithelial malignancies .…”

Section: Discussionmentioning

confidence: 99%

“…Reciprocal interactions between tumor and inflammatory cells can promote tumor progression . Among the various inflammatory cells found in the tumor microenvironment, macrophages are the most abundant component in many malignancies, including oral squamous cell carcinoma (SCC) . Macrophages can be phenotypically polarized by the microenvironment to create specific M1 or M2 functional programs .…”

Section: Introductionmentioning

confidence: 99%

“…In intraoral SCC, the nature and significance of MGC reactions are poorly understood. The few studies available suggest that MGC reactions represent a foreign body reaction to keratinized SCC cells and probably arise from M2 macrophages . In the context of lower lip SCCs, which differ in terms of pathogenesis and biological behavior from intraoral SCCs , to our knowledge, there are no previous studies evaluating the presence of MGC reaction and its relationship with tumor progression (PubMed Database, Scopus, Web of Science, LILACS, SIGLE).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

Santos

Miguel

Pinto

et al. 2017

J Oral Pathology Medicine

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Section: Discussionmentioning

confidence: 54%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

Santos

Miguel

Pinto

et al. 2017

J Oral Pathology Medicine

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“…And a recent study has also demonstrated that TAMs could be recruited by the tumor microenvironment of HNSCC [15]. However, the precise mechanism by which monocytes are recruited is still unclear.…”

Section: Resultsmentioning

confidence: 99%

CCL2/EGF positive feedback loop between cancer cells and macrophages promotes cell migration and invasion in head and neck squamous cell carcinoma

Gao

Wang

et al. 2016

Oncotarget

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Head and neck squamous cell carcinoma (HNSCC) represents the most frequent malignancy in the head and neck region, and the survival rate has not been improved significantly over the past three decades. It has been reported the infiltrated macrophages contribute to the malignant progression of HNSCC. However, the crosstalk between macrophages and cancer cells remains poorly understood. In the present study, we explored interactions between monocytes/macrophages and HNSCC cells by establishing the direct co-culture system, and found that the crosstalk promoted the migration and invasion of cancer cells by enhancing the invadopodia formation through a CCL2/EGF positive feedback loop. Our results demonstrated HNSCC cells educated monocytes into M2-like macrophages by releasing C-C motif chemokine ligand 2 (CCL2, or MCP-1). And the M2-like macrophages secreted epithelial growth factor (EGF), which increased the motility of HNSCC cells by enhancing the invadopodia formation. These subcellular pseudopodia degraded extracellular matrix (ECM), facilitating tumor local invasion and distant metastasis. Moreover, EGF up-regulated CCL2 expression in HNSCC cells, which recruited monocytes and turned them into M2-like macrophages, thus forming a positive feedback paracrine loop. Finally, we reported that curcumin, a powerful natural drug, suppressed the production of EGF and CCL2 in macrophages and cancer cells, respectively, blocking the feedback loop and suppressing the migration and invasion of HNSCC cells. These results shed light on the possibilities and approaches based on targeting the crosstalk between cancer cells and monocytes/macrophages in HNSCC for potential cancer therapy.

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CD163⁺ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia

Shigeoka

Koma

Nishio

et al. 2019

Clinical & Exp Dental Res

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ObjectiveAccumulating evidence suggests that macrophages are involved in the immunoediting of oral squamous cell carcinoma but the role of macrophages in oral carcinogenesis is unclear. We aimed to clarify the role of macrophages in oral leukoplakia, which is the most common oral potentially malignant disorder from immunotolerance viewpoint.Materials and methodsThe study included 24 patients who underwent surgical resection for tongue leukoplakia. The relationships between macrophage markers and clinicopathological factors were assessed. Conditioned medium was harvested from the CD163+ human monocytic leukaemia cell line, THP‐1. The phenotypic alteration of human oral keratinocytes by the conditioned medium treatment was assessed using quantitative reverse transcription‐polymerase chain reaction and enzyme‐linked immunosorbent assay. Moreover, the clinical samples were evaluated using immunohistochemistry.ResultsTongue leukoplakia tissues with high CD163+ macrophage infiltration were associated with significantly higher degrees of epithelial dysplasia, abnormal Ki‐67 expression and cytokeratin13 loss when compared with the tissues with low CD163+ macrophage infiltration. In vitro, CD163+ THP‐1 conditioned medium induced immunosuppressive molecules, especially interleukin‐10 (IL‐10) in human oral keratinocytes. The IL‐10 expression levels showed significant positive correlations with not only the numbers of FOXP3+ regulatory T cells but also that of CD163+ macrophages.ConclusionsIn tongue leukoplakia, CD163+ macrophages infiltration correlates with immunosuppressive cytokine IL‐10 expression.

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Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma

Cited by 16 publications

References 38 publications

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

CCL2/EGF positive feedback loop between cancer cells and macrophages promotes cell migration and invasion in head and neck squamous cell carcinoma

CD163⁺ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia

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Tumour-associated macrophages are recruited and differentiated in the neoplastic stroma of oral squamous cell carcinoma

Cited by 16 publications

References 38 publications

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

Multinucleated giant cell reaction in lower lip squamous cell carcinoma: a clinical, morphological, and immunohistochemical study

CCL2/EGF positive feedback loop between cancer cells and macrophages promotes cell migration and invasion in head and neck squamous cell carcinoma

CD163+ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia

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CD163⁺ macrophages infiltration correlates with the immunosuppressive cytokine interleukin 10 expression in tongue leukoplakia