Tumor viruses and early mouse embryos

Kelly, Françoise; Condamine, Hubert

doi:10.1016/0304-419x(82)90009-9

Cited by 41 publications

(30 citation statements)

References 189 publications

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“…Lanes A and B received DNA from 15 embryos; lanes C and D received DNA from 12 embryos; lanes E and F received DNA from 10 embroys; lanes G and H received DNA from 13 embryos; lanes I and J received DNA from 15 embryos; lanes K and L received DNA from 25 embryos; and lanes M and N received DNA from 15 embryos. cells are not permissive for PyV DNA replication (44). PyV DNA replication and expression of viral genes were not observed after infection of mouse embryos and mouse EC cells with PyV virions.…”

Section: -Cellmentioning

confidence: 91%

See 1 more Smart Citation

Sequence-dependent DNA replication in preimplantation mouse embryos.

Wirak¹,

Chalifour²,

Wassarman³

et al. 1985

Mol. Cell. Biol.

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Circular, double-stranded DNA molecules were injected into nuclei of mouse oocytes and one-or two-cell embryos to determine whether specific sequences were required to replicate DNA during mouse development.Although all of the injected DNAs were stable, replication of plasmid pML-l DNA was not detected unless it contained either polyomavirus (PyV) or simian virus 40 (SV40) DNA sequences. Replication occurred in embryos, but not in oocytes. PyV DNA, either alone or recombined with pML-l, underwent multiple rounds of replication to produce superhelical and relaxed circular monomers after injection into one-or two-cell embryos. SV40 DNA also replicated, but only 3% as well as PyV DNA. Coinjection of PyV DNA with either pML-1 or SV40 had no effect on the replicating properties of the three DNAs. These results are consistent with a requirement for specific cis-acting sequences to replicate DNA in mammalian embryos, in contrast to sequence-independent replication of DNA injected into Xenopus eggs. Furthermore, PyV DNA replication in mouse embryos required PyV large T-antigen and either the a-,8-core or ,-core configuration of the PyV origin of replication. Although the a-core configuration replicated in differentiated mouse cells, it failed to replicate in mouse embryos, demonstrating cell-specific activation of an origin of replication. Replication or expression of PyV DNA interfered with normal embryonic development. These results reveal that mouse embryos are permissive for PyV DNA replication, in contrast to the absence of PyV DNA replication and gene expression in mouse embryonal carcinoma cells.Although ori sequences have not yet been demonstrated as functional elements of eucaryotic chromosomes, they are implicated strongly by the temporal order of gene replication (11, 36); the selective amplification of specific genes (53,65,74); the existence of autonomously replicating sequences (26,76); and the fact that all bacterial, viral, plasmid, and organelle genomes require one or more unique cis-acting sequences that act as origins of replication (ori) and interact with specific gene products. However, some observations suggest that DNA replication during the early stages of embryonic development may not require sequence-specific origins of replication to carry out DNA replication.Analysis of DNA replication in Drosophila melanogaster (6,56,85) has revealed that the average distance between chromosome replication bubbles in preblastomere embryos is at least five times smaller than that in differentiated cells, suggesting that embryos initiate replication at many more sequences than do differentiated cells of the same species. Furthermore, injection of DNA into Xenopus eggs revealed that semiconservative DNA replication can occur under the apparent control of the cell division cycle but with no apparent requirement for specific DNA sequences (34,57,58). All of the DNA molecules examined, including simian virus 40 (SV40) and polyomavirus (PyV) DNA which normally replicate only in differentiated cells of a specific m...

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Section: -Cellmentioning

confidence: 91%

“…Although PyV replicates in a variety of differentiated mouse cells (25), neither mouse embryos (44) nor mouse embryonal carcinoma (EC) cells have been reported to support PyV DNA transcription (19,31,71) or replication (18,30). EC cells share many properties found in multipotent cells of 7-to 8-day-old mouse embryos (54).…”

mentioning

confidence: 99%

Sequence-dependent DNA replication in preimplantation mouse embryos.

Wirak¹,

Chalifour²,

Wassarman³

et al. 1985

Mol. Cell. Biol.

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“…It has been shown that only differentiated cells support viral replication, while undifferentiated cells are not permissive (Kelly and Condamine, 1982). The lack of virus growth in undifferentiated cells has been related to a block in early viral mRNA synthesis.…”

Section: Introductionmentioning

confidence: 99%

Selection of mouse neuroblastoma cell-specific polyoma virus mutants with stage differentiative advantages of replication.

Maione¹,

Passananti²,

Simone³

et al. 1985

The EMBO Journal

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Two mouse neuroblastoma cell lines were analyzed for their permissivity for polyoma virus growth. One (N18) is fully permissive for polyoma replication, the other (41A3) shows limited permissivity and the viral genome persists, without noticeable cell death. Virus persistence does not seem to alter the cells' ability to differentiate in vitro and leads to selection of viral mutants altered in the untranscribed regulatory region of the genome. The mutant types obtained appear to be related to the degree of host cell differentiation. Nucleotide sequence analysis of the restriction fragment covering the regulatory region shows that duplications are present in all mutants, while deletions in the non-duplicated segment are only present in mutants selected from less differentiated cells. These alterations involve both domains of the regulatory region that are considered to be essential for DNA replication and for enhancer activity. Mixed infections with polyoma wild type show that the selected mutants have cis-advantage in replication in neuroblastoma cells and not in 3T6 cells. Mutants carrying the deletion in the non-duplicated segment of the enhancer show a selective advantage in replication over the undeleted one in mixed infection. This advantage is much stronger in neuroblastoma cells in suspension (less-differentiated stage) than in monolayer cells (more-differentiated stage). An interpretation of the overall structure of the regulatory enhancer region, based on the observed differences between the mutants selected at different stages of differentiation in neuroblastoma and previously described mutants selected in undifferentiated cells, is discussed.

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“…IAPs are regularly observed by electron microscopy in mouse oocytes and early embryos (reviewed in references 15,46,47), and both IAP-specific proteins (12)(13)(14) and RNA (37) have been demonstrated in preimplantation embryos. IAPs were seen only occasionally in a systematic survey of normal mouse tissues that included adult BALB/c thymus (45).…”

mentioning

confidence: 99%

Intracisternal A-particle gene expression in normal mouse thymus tissue: gene products and strain-related variability.

Kuff¹,

Fewell²

1985

Mol. Cell. Biol.

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Intracisternal A-particle (IAP)-specific sequences were 5-to 10-fold enriched in polyadenylated RNA from BALB/cJ thymus as compared with RNAs from liver, spleen, and kidney. The major transcripts of 7.2 and 5.4 kilobases were the same size as those found in an TAP-rich neuroblastoma cell line. The absolute levels and proportions of these transcripts varied in thymuses from mice of different inbred strains. With antiserum prepared against p73, the main IAP structural protein, several size classes of IAP-related proteins were immunoprecipitated from extracts of thymus cells incubated with [35S]methionine; these included p73 itself and a group of polypeptides in the size range of 114 to 120 kilodaltons (pll4-pl20). The inbred strains showed marked characteristic differences in the electrophoretic patterns of their IAP-related proteins. Earlier studies showed that the 7.2-kilobase RNA from neuroblastoma IAPs coded for p73 in a cell-free translation system. Correlations between the RNA and protein patterns in thymuses of the different inbred strains indicated that 5.4-kilobase RNA gives rise to the pll4-pl20 polypeptides. Metabolically labeled p120 was found to include methionine-containing tryptic peptides of p73 plus additional peptides consistent with its larger size. In vivo labeling kinetics showed that the pll4-pl20 polypeptides were not major precursors of p73 in intact neuroblastoma cells. This study shows that IAP gene expression in mouse thymus is genetically determined and that a novel class of IAP-related polypeptides can be expressed independently of the major particle structural protein.Intracisternal A-particles (IAPs) are retrovirus-like entities found in many types of mouse tumor cells (20,45). They contain a group-specific internal structural protein, p73 (20,32), and a DNA polymerase activity with properties of reverse transcriptase (42, 43). The isolated particles also contain specific polyadenylated [poly(A)] RNAs (30) ranging in size from 7.2 to about 3.5 kilobases (kb) (34,36,41). These RNA species, which are related to one another in sequence, vary in relative proportion in particles isolated from different tumor sources. Endogenous provirus-like elements homologous to the IAP-associated RNAs ("IAP genes") are reiterated 1,000-fold per haploid genome in Mus musculus (28). The full-size genetic unit is 7.3 kb in length and is colinear with the 7.2-kb transcript (21). Shorter elements with internal deletions are also found (21, 34), and one group of these, the so-called type IT elements (40), are known to give rise to some of the shorter IAP-specific RNAs (34, 41). The IAP genetic elements share many structural properties with integrated retroviral proviruses and certain transposable elements in Drosophila spp. and yeasts (8a). Recently, IAP genes have been found to appear in novel locations in the genome of certain IAP-rich BALB/c mouse myeloma and hybridoma cell lines and to affect the function of genes at the various target sites (5,7,9,10,18,19,41). Thus, on occasion they can act as mobile e...

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Tumor viruses and early mouse embryos

Cited by 41 publications

References 189 publications

Sequence-dependent DNA replication in preimplantation mouse embryos.

Sequence-dependent DNA replication in preimplantation mouse embryos.

Selection of mouse neuroblastoma cell-specific polyoma virus mutants with stage differentiative advantages of replication.

Intracisternal A-particle gene expression in normal mouse thymus tissue: gene products and strain-related variability.

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