Tumor-targeting [2]catenane-based grid-patterned periodic DNA monolayer array for<i>in vivo</i>theranostic application

Chen, Yanru; Sun, Shaoli; Yin, Hongwei; Liu, Ran; Xu, Huo; Yang, Ya; Wu, Zai‐Sheng

doi:10.1039/d1tb01978c

Cited by 5 publications

(2 citation statements)

References 124 publications

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“…As shown in Figure S1A, naked AuNPs are nearly spherical and monodispersed, and they have an average height (AH) of 15.9 ± 0.8 nm and average width (AW) of 31.9 ± 5.9 nm. The fact that the AH value is lower than AW is reasonable taking the AFM tip-broadening effect into account. , The AFM-measured value of naked AuNPs is consistent with the literature reports . From AFM in Figure S1B, there is a yellowish halo surrounding each particle, and an increase in the size of nanoparticles is detected.…”

Section: Resultssupporting

confidence: 90%

“…The fact that the AH value is lower than AW is reasonable taking the AFM tip-broadening effect into account. 40,41 The AFMmeasured value of naked AuNPs is consistent with the literature reports. 42 From AFM in Figure S1B, there is a yellowish halo surrounding each particle, and an increase in the size of nanoparticles is detected.…”

Section: ■ Results and Discussionsupporting

confidence: 90%

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Spherical Nucleic Acid-Mediated Spatial Matching-Guided Nonenzymatic DNA Circuits for the Prediction and Prevention of Malignant Tumor Invasion

Wang,

Li,

Luo

et al. 2024

Anal. Chem.

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Detection of intracellular miRNAs, especially sensitive imaging of in vivo miRNAs, is vital to the precise prediction and timely prevention of tumorgenesis but remains a technical challenge in terms of nuclease resistance and signal amplification. Here, we demonstrate a gold nanoparticle-based spherical nucleic acid-mediated spatial matchingguided nonenzymatic DNA circuit (SSDC) for efficient screening of intracellular miRNAs and, in turn, finding cancerous tissues in living organisms before the appearance of clinical symptoms. Due to the substantially enhanced nuclease resistance, the false positive signal is avoided even in a complex biological medium. Target miRNA can straighten out the hairpin DNA probe to be linear, allowing the probe to penetrate into the internal region of a core/ shell DNA-functionalized signal nanoampfilier and initiate a strand displacement reaction, generating an amplified fluorescence signal. The detection limit is as low as 17 pM, and miRNA imaging is in good accordance with the gold standard polymerase chain reaction method. The ability to image intracellular miRNAs is substantially superior to that of conventional fluorescence in situ hybridization techniques, making in vivo SSDC-based imaging competent for the precise prediction of tumorigenesis. By intratumoral chemotherapy guided by SSDC-based imaging, tumorigenesis and progression are efficiently controlled before the onset of clinical symptoms.

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Section: Resultssupporting

confidence: 90%

Section: ■ Results and Discussionsupporting

confidence: 90%

Spherical Nucleic Acid-Mediated Spatial Matching-Guided Nonenzymatic DNA Circuits for the Prediction and Prevention of Malignant Tumor Invasion

Wang,

Li,

Luo

et al. 2024

Anal. Chem.

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Intelligent Reconfiguration‐Promoted Cellular Internalization of Core–Shell DNA Nanoprobe Equipped with Successive Dual Stimuli‐Responsive Protective Satellites for Amplification Fluorescence Imaging of Tumor Cells

Pan,

Niu,

Luo

et al. 2024

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Although DNA probes have attracted increasing interest for precise tumor cell identification by imaging intracellular biomarkers, the requirement of commercial transfection reagents, limited targeting ligands, and/or non‐biocompatible inorganic nanostructures has hampered the clinic translation. To circumvent these shortcomings, a reconfigurable ES‐NC (Na+‐dependent DNAzyme (E)‐based substrate (S) cleavage core/shell DNA nanocluster (NC)) entirely from DNA strands is assembled for precise imaging of cancerous cells in a successive dual‐stimuli‐responsive manner. This nanoprobe is composed of a strung DNA tetrahedral satellites‐based protective (DTP) shell, parallelly aligned target‐responsive sensing (PTS) interlayer, and hydrophobic cholesterol‐packed innermost layer (HCI core). Tetrahedral axial rotation‐activated reconfiguration of DTP shell promotes the exposure of interior hydrophobic moieties, enabling cholesterol‐mediated cellular internalization without auxiliary elements. Within cells, over‐expressed glutathione triggers the disassembly of the DTP protective shell (first stimulus), facilitating target‐stimulated signal transduction/amplification process (second stimuli). Target miRNA‐21 is detected down to 10.6 fM without interference from coexisting miRNAs. Compared with transfection reagent‐mediated counterpart, ES‐NC displays a higher imaging ability, resists nuclease degradation, and has no detectable damage to healthy cells. The blind test demonstrates that the ES‐NC is suitable for the identification of cancerous cells from healthy cells, indicating a promising tool for early diagnosis and prediction of cancer.

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Center backbone-rigidified DNA polygonal nanostructures and bottom face-templated polyhedral pyramids with structural stability in a complex biological medium

et al. 2023

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Tumor-targeting [2]catenane-based grid-patterned periodic DNA monolayer array forin vivotheranostic application

Abstract: By cross-catenating two DNA rings containing palindromic fragments, we demonstrate a catenane-based grid-patterned periodic DNA monolayer array ([2]GDA) capable of accumulating in tumor tissues and amenable to the delivery of anticancer drugs.

Cited by 5 publications

References 124 publications

Spherical Nucleic Acid-Mediated Spatial Matching-Guided Nonenzymatic DNA Circuits for the Prediction and Prevention of Malignant Tumor Invasion

Spherical Nucleic Acid-Mediated Spatial Matching-Guided Nonenzymatic DNA Circuits for the Prediction and Prevention of Malignant Tumor Invasion

Intelligent Reconfiguration‐Promoted Cellular Internalization of Core–Shell DNA Nanoprobe Equipped with Successive Dual Stimuli‐Responsive Protective Satellites for Amplification Fluorescence Imaging of Tumor Cells

Center backbone-rigidified DNA polygonal nanostructures and bottom face-templated polyhedral pyramids with structural stability in a complex biological medium

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