Tumor Suppressor MicroRNA-27a in Colorectal Carcinogenesis and Progression by Targeting SGPP1 and Smad2

Bao, Yonghua; Chen, Zhiguo; Guo, Yanning; Feng, Yan; Li, Zexin; Han, Wenliang; Wang, Jianguo; Zhao, Weixing; Jiao, Yunjuan; Li, Kai; Wang, Qian; Wang, Jiaqi; Zhang, Huijuan; Wang, Liang; Yang, Wancai

doi:10.1371/journal.pone.0105991

Cited by 96 publications

(93 citation statements)

References 45 publications

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“…We selected the SMAD2 and SMAD4 genes as potential targets of miR‐27a in lung cancer. Recent studies have also reported that miR‐27a regulated SMAD2 and SMAD4 in human lung fibroblasts and SGPP1 and SMAD2 in human colorectal cancer . In addition, upregulation of miR‐27a has been reported in a number of cancers, such as breast cancer, gastric adenocarcinoma, pancreatic carcinoma, and lung cancer, describing it as an oncogene.…”

Section: Discussionmentioning

confidence: 93%

MIR‐27a regulates the TGF‐β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer

Chae

Ban

Yoo

et al. 2017

Molecular Carcinogenesis

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The transforming growth factor-β (TGF-β) signaling pathway is associated with carcinogenesis and various biological processes. SMAD2 and SMAD4, which are putative tumor suppressors, have an important role in TGF-β signaling. The aberrant expression of these genes is implicated in some cancers. However, the mechanisms of SMAD2 and SMAD4 dysregulation are poorly understood. In this study, we observed that miR-27a was upregulated in lung cancer cell lines and patients. In addition, SMAD2 and SMAD4 genes were identified as targets of miR-27a by several target prediction databases and experimental validation. Functional studies revealed that miR-27a overexpression decreased SMAD2 and SMAD4 mRNA and protein levels. Furthermore, miR-27a contributed to cell proliferation and invasion by inhibiting TGF-β-induced cell cycle arrest. These results suggest that miR-27a may function as an oncogene by regulating SMAD2 and SMAD4 in lung cancer. Thus, miR-27a may be a potential target for cancer therapy.

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Section: Discussionmentioning

confidence: 93%

MIR‐27a regulates the TGF‐β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer

Chae

Ban

Yoo

et al. 2017

Molecular Carcinogenesis

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“…These oncomiRs effect CRC cell proliferation, apoptosis, migration and invasion (12). However, another type of miRNA, termed tumor suppressive miRs (tsmiRs), are significantly downregulated in human colorectal adenocarcinoma tissue samples compared with the adjacent normal colorectal tissues, including miR-339-5p, miR-27a and miR-139-5p (13)(14)(15). miR-517a is considered to be a novel oncomiR and was observed to be elevated in human hepatocellular carcinoma (HCC) (16); expression of miR-517a increased proliferation, migration, and invasion of HCC cells in vitro (16).…”

Section: Introductionmentioning

confidence: 99%

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Jiang

et al. 2016

Oncology Letters

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Abstract. Colorectal cancer (CRC) is a highly invasive tumor that is frequently associated with distant metastasis, which is the primary cause of poor prognosis. However, the mechanisms of metastasis remain poorly understood. MicroRNAs (miRNAs/miRs) have been considered to be implicated in CRC progression. In particular, miR-517a is proposed as a novel tumor-associated miRNA and has a potential role in tumor metastasis. The expression of miR-517a in CRC specimens was detected by reverse transcription-quantitative polymerase chain reaction. Transwell assays were performed to determine the migration and invasion of CRC cells. The putative target genes of miR-517a were disclosed using publicly available databases and western blot analysis. The present study identified that the expression of miR-517a was significantly higher in CRC tissues as compared with adjacent non-tumor tissues. Clinical analysis indicated that increased expression of miR-517a was correlated with poor prognostic features and poor long-term survival of CRC patients. In vitro evidences demonstrated that downregulation of miR-517a inhibited cell migration and invasion in HCT-116 cells. By contrast, upregulation of miR-517a increased the number of migrated and invaded SW480 cells. Notably, miR-517a expression was inversely regulated by forkhead box J3 (FOXJ3) abundance in CRC cells. Furthermore, an inverse correlation between miR-517a and FOXJ3 expression was observed in CRC tissues. In conclusion, miR-517a appears to be an independent prognostic marker for predicting survival of CRC patients, and may promote cell migration and invasion by inhibiting FOXJ3.

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“…Accumulating evidence has shown that miRNA might play critical roles in cancer initiation and the progression processes, including DLBCL . MiR‐27a, which located at chromosome 19, has been reported to be associated with several types of cancer, such as colon cancer, breast cancer, gastric adenocarcinoma, and pancreatic carcinoma . In addition, several studies have investigated the relationship between Pre‐miR‐27a rs895819 polymorphism and risk of cancer such as colorectal cancer, cervical cancer, and gastric cancer .…”

Section: Introductionmentioning

confidence: 99%

Pre‐miR‐27a rs895819 polymorphism and risk of diffuse large B‐cell lymphoma

Tang

et al. 2019

Clinical Laboratory Analysis

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Background Recently, several studies have investigated the relationship between Pre‐miR‐27a rs895819 polymorphism and risk of various cancers. However, the relationship between rs895819 and diffuse large B‐cell lymphoma (DLBCL) has not been well known. Methods In this study, we conducted a case‐control study to explore the role of Pre‐miR‐27a rs895819 in risk of DLBCL. The PCR‐TaqMan and luciferase assays and in vitro experiments were used to evaluate polymorphism function. Results As a result, we found subjects carrying with rs895819 AG/GG genotype had a significantly decreased risk when compared with those carrying the AA genotype. Further qPCR assay showed that the DLBCL patients carrying AG/GG genotypes showed a lower level of mature miR‐27a when compared with patients carrying AA genotype. Moreover, miR‐27a levels were upregulated in DLBCL tissues compared with normal lymphoid tissues. Further in vitro experiments showed that miR‐27a might function as an oncogene through target TGFBR1. In addition, TGFBR1 overexpression rescues effects of miR‐27a inhibitor on DLBCL cells phenotypes. Conclusions In conclusion, these findings indicate that rs895819 A > G might reduce the expression of mature miR‐27a, and leading a higher level of TGFBR1, ultimately inhibiting the development of DLBCL.

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Tumor Suppressor MicroRNA-27a in Colorectal Carcinogenesis and Progression by Targeting SGPP1 and Smad2

Cited by 96 publications

References 45 publications

MIR‐27a regulates the TGF‐β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer

MIR‐27a regulates the TGF‐β signaling pathway by targeting SMAD2 and SMAD4 in lung cancer

miR-517a is an independent prognostic marker and contributes to cell migration and invasion in human colorectal cancer

Pre‐miR‐27a rs895819 polymorphism and risk of diffuse large B‐cell lymphoma

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