Tumor suppressor microRNA-198 is actively transported out of liver cancer cells

Yu, Xiaojie; Eischeid, H; Büttner, Reinhard; Odenthal, Margarete

doi:10.1055/s-0036-1597498

Cited by 1 publication

(1 citation statement)

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“…Interestingly, during liver diseases, increased miR-198 levels can be found in the blood stream while the intracellular expression is significantly downregulated. It has been recently revealed that miR-198 is actively transported out of liver cancer cells via exocytosis 15 , although proteins responsible for this transport are yet unknown. Therefore, we also identified proteins attached to miR-198 capturing beads that provided new insights to elucidate the bioanalytical potential of this new process.…”

Section: Resultsmentioning

confidence: 99%

Selective Capture and Purification of MicroRNAs and Intracellular Proteins through Antisense-vectorized Magnetic Nanobeads

Gessner

Jungreuthmayer

et al. 2019

Sci Rep

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MicroRNAs (miRNAs) are small non-coding nucleotides playing a crucial role in posttranscriptional expression and regulation of target genes in nearly all kinds of cells. In this study, we demonstrate a reliable and efficient capture and purification of miRNAs and intracellular proteins using magnetic nanoparticles functionalized with antisense oligonucleotides. For this purpose, a tumor suppressor miRNA (miR-198), deregulated in several human cancer types, was chosen as the model oligonucleotide. Magnetite nanoparticles carrying the complementary sequence of miR-198 (miR-198 antisense) on their surface were delivered into cells and subsequently used for the extracellular transport of miRNA and proteins. The successful capture of miR-198 was demonstrated by isolating RNA from magnetic nanoparticles followed by real-time PCR quantification. Our experimental data showed that antisense-coated particles captured 5-fold higher amounts of miR-198 when compared to the control nanoparticles. Moreover, several proteins that could play a significant role in miR-198 biogenesis were found attached to miR-198 conjugated nanoparticles and analyzed by mass spectrometry. Our findings demonstrate that a purpose-driven vectorization of magnetic nanobeads with target-specific recognition ligands is highly efficient in selectively transporting miRNA and disease-relevant proteins out of cells and could become a reliable and useful tool for future diagnostic, therapeutic and analytical applications.

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Section: Resultsmentioning

confidence: 99%