Tumor suppressor Interferon Regulatory Factor 1 selectively blocks expression of endogenous retrovirus

Stoltz, Kyle; Jondle, Christopher Ned; Pulakanti, Kirthi; Sylvester, P. A.; Urrutia, Raúl; Rao, Sridhar; Tarakanova, Vera L.

doi:10.1016/j.virol.2018.10.003

Cited by 8 publications

(6 citation statements)

References 64 publications

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“…DNase-treated total RNA (1 mg) was reverse transcribed into cDNA with Omniscript RT kit (QIAGEN) following manufacturer’s instructions. qPCR was performed using the iQ SYBR Green Supermix (Life Technologies) on a QuantStudio 6 Flex Real-Time PCR (Life Technologies) using the previously published primer sequences listed ( Table S1 ; Collins et al., 2015 ; Karimi et al., 2011 ; Macfarlan et al., 2011 ; Stoltz et al., 2019 ; Yin et al., 2011 ; Yoshinobu et al., 2009 ; Young et al., 2012b ). The housekeeping gene Gapdh was used to normalize the Critical Threshold (CT) values.…”

Section: Methodsmentioning

confidence: 99%

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Lima-Júnior

Krishnamurthy

Bouladoux

et al. 2021

Cell

112

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Section: Methodsmentioning

confidence: 99%

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Lima-Júnior

Krishnamurthy

Bouladoux

et al. 2021

Cell

112

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“…The tendency for alteration in the transcription factors also affects the two transcription factors, irf1 and irf3 tendencies for binding. These transcription factors are interferon regulator factors with tumor suppression effect in addition to activation of the innate immunity apoptosis and response to DNA damage for the factor irf1 [ 38 , 39 ]. IRF3 is associated with the activation of the transcription of interferon’s alpha and beta, as well as other interferon-induced genes [ 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Study of single nucleotide polymorphism of vascular endothelium factor in patients with differentiated thyroid cancer

Motawea

Zaki

Saif

et al. 2022

Clin Diabetes Endocrinol

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Background Genetic alterations and high levels of the vascular endothelial growth factor (VEGF) are presumptive risk factors for differentiated thyroid cancer (DTC). Objective This work aims to study the presence of − 634G/C polymorphism of vascular endothelial growth factor (rs2010963) and its’ serum level in patients with DTC and comparing these results with those of the control subjects. Material and method The study was a retrograde case–control study that included seventy patients with DTCin addition to seventy apparently healthy control subjects. Blood sample was taken and subjected to study of − 634G/C VEGF polymorphism (rs2010963) by real time PCR and measurement of its’ plasma level by immunoassay kit (ELISA). Results Regarding genotyping of VEGFA − 634G/C (rs2010963) polymorphism, there was significant increase in CG and GG genotypes (28.6%, 18.6% respectively) among patients compared to control subjects (20.0%, 4.3% respectively) and significant increase in CC genotype in control subjects (75.7%) compared to patients (52.9%), P = 0.001. The VEGF mean ± SD level was significantly elevated in patients compared to control subjects (1215.81 ± 225.78 versus 307.16 ± 91.81, P = 0.006). Moreover, there was significant increase in VEGF levels in patients with CG and GG genotypes (1295.9 ± 68.74, 1533.08 ± 109.95, respectively) compared to patients with CC genotype (1061 163.25), P = 0.001). Conclusion There was significant increase in GG and CG genotypes in patients with DTC compared to control subjects which may suggest a predisposing role for these genotypes in development of DTC. Moreover, there was significant increase in serum level of vascular endothelial growth factor in patients with GG and CG genotypes which may reflect the mechanism of these genotypes in development of DTC.

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“…Activity of untargeted dCAS9 had a strong effect on gene expression, potentially because of DNA damage induction and off-target effects ( Pluripotency is associated with lack of epigenetic repression and transcriptional activation of transposable elements (TEs; Peaston et al, 2004). Transposable elements include endogenous retroviruses, and IRF1 is involved in repression of endogenous retroviruses (Stoltz et al, 2019). We analyzed expression levels of TE families in WT differentiation and in the absence of IRF1 in EpiLCs (Dataset EV6).…”

Section: Irf1 Is a Regulator Of Interferon-stimulated Genes During Pl...mentioning

confidence: 99%

Transient upregulation of IRF1 during exit from naive pluripotency confers viral protection

et al. 2022

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Stem cells intrinsically express a subset of genes which are normally associated with interferon stimulation and the innate immune response. However, the expression of these interferon-stimulated genes (ISG) in stem cells is independent from external stimuli such as viral infection. Here, we show that the interferon regulatory factor 1, Irf1, is directly controlled by the murine formative pluripotency gene regulatory network and transiently upregulated during the transition from naive to formative pluripotency. IRF1 binds to regulatory regions of a conserved set of ISGs and is required for their faithful expression upon exit from naive pluripotency. We show that in the absence of IRF1, cells exiting the naive pluripotent stem cell state are more susceptible to viral infection. Irf1 therefore acts as a link between the formative pluripotency network, regulation of innate immunity genes, and defense against viral infections during formative pluripotency.

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Tumor suppressor Interferon Regulatory Factor 1 selectively blocks expression of endogenous retrovirus

Cited by 8 publications

References 64 publications

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Endogenous retroviruses promote homeostatic and inflammatory responses to the microbiota

Study of single nucleotide polymorphism of vascular endothelium factor in patients with differentiated thyroid cancer

Transient upregulation of IRF1 during exit from naive pluripotency confers viral protection

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