Tumor suppression by resistant maltodextrin, Fibersol-2

Abstract

Abbreviations: BAX, Bcl-2-associated X protein; MFI, mean fluorescence intensity; mTOR, mammalian target of rapamycin; PARP, poly ADP reibose polymerase; ROS, reactive oxygen speciesResistant maltodextrin Fibersol-2 is a soluble and fermentable dietary fiber that is Generally Recognized As Safe (GRAS) in the United States. We tested whether Fibersol-2 contains anti-tumor activity. Human colorectal cancer cell line, HCT116, and its isogenic cells were treated with FIbersol-2. Tumor growth and tumorigenesis were studied in vitro and in vivo. Apoptotic pathway and generation of reactive oxygen species (ROS) were investigated. We discovered that Fibersol-2 significantly inhibits tumor growth of HCT116 cells by inducing apoptosis. Fibersol-2 strongly induces mitochondrial ROS and Bax-dependent cleavage of caspase 3 and 9, which is shown by isogenic HCT116 variants. Fibersol-2 induces phosphorylation of Akt, mTOR in parental HCT116 cells, but not in HCT116 deficient for Bax or p53. It prevents growth of tumor xenograft without any apparent signs of toxicity in vivo. These results identify Fibersol-2 as a mechanism-based dietary supplement agent that could prevent colorectal cancer development.

The Novel Herbal Cocktail AGA Alleviates Oral Cancer through Inducing Apoptosis, Inhibited Migration and Promotion of Cell Cycle Arrest at SubG1 Phase

“…In addition to this, the gluconate may be partially attributed to an increased anti-cancer impact when combined with other therapeutics, such as Zn2+ [22]. Furthermore, maltodextrin also imparts tumor suppression by inducing apoptosis via phosphorylation of Akt [23]. Based on our therapeutics evidenced on antrodia camphorata, we synthesized a robust cocktail, i.e., AGA, in addition to minor quantities of L-arginine, Zinc gluconate and maltodextrin, to investigate its anti-oral cancer potential.…”

The Effects of Prebiotic Supplementation with OMNi-LOGiC® FIBRE on Fecal Microbiome, Fecal Volatile Organic Compounds, and Gut Permeability in Murine Neuroblastoma-Induced Tumor-Associated Cachexia

“…OMNi-LOGiC ® FIBRE is a prebiotic consisting of dextrin (resistant starch type 4 with α-1,2- and α-1,3-linked dextrose) and partially hydrolyzed guar gum (β-1,4-linked mannose with side chains of α-1,6-linked galactose). In previous studies, dextrin increased the amount of cecal SCFAs and inhibited tumor growth in cell cultures and tumor development in mouse xenografts of HCT116 cell lines [ 29 ]. Guar gum lead to an increase of cecal SCFA, a reduction of pro-inflammatory cytokines, and an improved gut barrier in models of experimental colitis [ 30 , 31 ].…”

“…Dextrin has been reported to induce mitochondrial production of reactive oxygen species and Bax-dependent cleavage of caspases 3 and 9, leading to decreased tumor cell growth in HCT116 (human colorectal carcinoma) cell cultures and to reduced tumor development in HCT116 mouse xenografts [ 29 ]. In contrast, our NB-bearing animals did not react to this substance regarding tumor take rate, tumor growth, or tumor weight.…”

Efficiency of Resistant Starch and Dextrins as Prebiotics: A Review of the Existing Evidence and Clinical Trials

“…5 or 10 g 4 h A total of 10 g of Fibersol-2 stimulates production of satiety hormones (peptide-YY). [140] Fibersol-2 HTC116 cell line --Significant inhibition of tumor growth of HCT116 cells by induction of apoptosis without visible signs of toxicity in vivo.…”