Tumor-Suppressing Effects of miR-141 in Human Osteosarcoma

Abstract

Osteosarcoma is the most common primary malignancy to arise from bone. The pathogenesis of osteosarcoma is unclear, and new therapy molecular target is needed. The miRNAs researched suggested that miRNAs are involved in the pathogenesis of osteosarcoma. MiR-141, which belong to miR-200 family, take a part in tumorigenesis. However, the role of miR-141 in the pathogenesis of osteosarcoma remained unclear. In this study, we focused on the miR-141 in osteosarcoma and found that the expression of miR-141 is lower in osteosarcoma. Overexpression of miR-141 not only inhibits osteosarcoma cell proliferation but also induces cell apoptosis. It is estimated that miR-141 played its role via ZEB1 and ZEB2. In all, miR-141 played a osteosarcoma-suppressing role via ZEB1 and ZEB2. Our finding may elucidate the miRNAs mechanism in osteosarcoma and provide a new molecule target for osteosarcoma therapy.

Long Non-coding RNA HOTAIR Is Targeted and Regulated by miR-141 in Human Cancer Cells

“…4A). We also examined whether miR-141 alters the transcription activities on the HOTAIR promoter because miR-141 regulates transcription factors such as ZEB1 and ZEB2 (23,35,36). Treatment with ␣-amanitin, an inhibitor of RNA polymerase II, did not significantly affect miR-141 suppression of HOTAIR in 786-O and ACHN cells (Fig.…”

“…We also examined whether miR-141 transcriptionally suppresses HOTAIR because miR-141 regulates transcription factors, such as ZEB1 and ZEB2 (23,35,36). We treated 786-O and ACHN cells with ␣-amanitin, which suppresses RNA polymerase II expression and activity (37, 38) and examined HOTAIR expression after transfection of miR-141.…”

miR-574-3p acts as a tumor promoter in osteosarcoma by targeting SMAD4 signaling pathway

“…Surgical specimens from 10 osteosarcoma and matched normal control tissues were obtained postoperatively in May 2010 from the Department of Orthopedics of Jinling Hospital, Nanjing University, School of Medicine (Nanjing, China) (3,5,10). All patients provided signed informed consent for their tissues to be used for scientific research.…”

“…Ethical approval for the study was obtained from Jinling Hospital, Nanjing University. All diagnoses were based on pathological and/or cytological evidence (10). The histological features of the specimens were evaluated by senior pathologists according to the World Health Organization classification criteria (11)(12)(13).…”

“…The critical functions of numerous miRNAs in cancer biology have been illustrated, including the regulation of various cellular processes, such as proliferation and apoptosis (3,5,9,10). The abnormality of miR-574-3p was observed in patients with non-small cell lung cancer and prostate cancer, and it was found that this miRNA regulated colorectal cancer growth and plays important roles in bladder cancer cell lines (11)(12)(13).…”