Tumor–stroma interactions influence cytokine expression and matrix metalloproteinase activities in paired primary and metastatic head and neck cancer cells

Koontongkaew, Sittichai; Amornphimoltham, Panomwat; Yapong, Bunjird

doi:10.1016/j.cellbi.2008.10.009

Cited by 32 publications

(33 citation statements)

References 26 publications

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“…Similarly, direct injection of human MSC into ischemic hearts decreased fibrosis, left ventricular dilation, apoptosis, and increased myocardial thickness with preservation of systolic and diastolic cardiac function without evidence of myocardial regeneration. MSCs appear to achieve this improved function by increasing acutely the cellularity and decreasing production of extracellular (25,35) .…”

Section: B4) Beneficial Remodeling Of the Extracellular Matrixmentioning

confidence: 99%

“…Evidence also exists that both endogenous and exogenous stem cells are able to "home" or migrate into the area of injury from the site of injection or infusion (26,30,(32)(33)(34)(35)(36)(37)(38) . MSC in the bone marrow can be mobilized, target the areas of infarction, and differentiate into target tissue type.…”

Section: B3) Anti-apoptotic and Chemotactic Signalingmentioning

confidence: 99%

“…Fourth, stem cell transplantation alters the extracellular matrix, resulting in more favorable post-infarct remodeling, strengthening of the infarct scar, and prevention of deterioration in organ function (34)(35)(36)(37) . Acute human and murine MSC infusion prior to ischemia improve myocardial developed pressure, contractility, and compliance after ischemia/reperfusion (I/R) injury and decrease end diastolic pressure.…”

Section: B4) Beneficial Remodeling Of the Extracellular Matrixmentioning

confidence: 99%

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Possible mechanisms of retinal function recovery with the use of cell therapy with bone marrow-derived stem cells

Siqueira¹,

Voltarelli²,

Messias³

et al. 2010

Arq. Bras. Oftalmol.

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Section: B4) Beneficial Remodeling Of the Extracellular Matrixmentioning

confidence: 99%

Section: B3) Anti-apoptotic and Chemotactic Signalingmentioning

confidence: 99%

Section: B4) Beneficial Remodeling Of the Extracellular Matrixmentioning

confidence: 99%

See 1 more Smart Citation

Possible mechanisms of retinal function recovery with the use of cell therapy with bone marrow-derived stem cells

Siqueira¹,

Voltarelli²,

Messias³

et al. 2010

Arq. Bras. Oftalmol.

View full text Add to dashboard Cite

“…CAFs have been induced from regular fibroblasts, and even from mesenchymal stem cells, via treatment with carcinoma cell-derived medium (17) or a co-culture model (18). In addition, previous studies have demonstrated that co-culture with CAFs changes the level of secretion of numerous cytokines from carcinoma cells (19,20), thus, giving them a higher potential for invasion and metastasis. The possibility of the two cell types mutually influencing each other is one of the advantages of the Transwell co-culture model, and the background for selecting it for use in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…Various studies have shown that patients with HNSCC commonly overexpress cytokines, including IL-1, IL-6, tumor necrosis factor (TNF)-α and TNF-β (25,26). Among others, IL-6 has been shown to be elevated in HNSCC cell lines of metastatic origin compared with those cultivated from the primary tumor lesion (19). Therefore, an IL-6 assay was integrated into the present study, as increased levels of IL-6 indicate a higher potential for tumor proliferation and metastasis.…”

Section: Discussionmentioning

confidence: 99%

Differences in tumor stroma derived from irradiated versus non-irradiated fibroblasts in a co-culture model with head and neck squamous cell carcinoma

et al. 2016

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Abstract. Cancer cells have a variety of interactions with neighboring connective tissue, and this activity primarily involves fibroblasts. Co-culture of fibroblasts derived from human skin with cancer cells results in the conversion of fibroblasts into cancer-associated fibroblasts, which are known to support tumor growth and invasiveness. To evaluate the effect of radiation on tumor-fibroblast interactions, the present study co-cultivated fibroblasts from pre-irradiated and non-irradiated human skin with FaDu head and neck squamous cell carcinoma cells for 3 days. Subsequently, cells were analyzed for tumor viability, apoptosis, and secretion of interleukin (IL)-6 and -8 by performing an MTT assay, Annexin V-propidium iodide test and enzyme-linked immunosorbent assay, respectively. Co-culture of FaDu cells with pre-irradiated fibroblasts resulted in a significant decrease in tumor viability, a notable increase in apoptosis and significantly lower levels of IL-8 compared with FaDu cells cultured with non-irradiated fibroblasts. Therefore, we propose that pre-irradiation changes the properties of fibroblasts and their effects on co-cultivated tumor cells, and, thus may lead to an improved understanding of the therapeutic options for patients that have already undergone irradiation. IntroductionSquamous cell carcinoma is a major cause of cancer morbidity and mortality, and is one of the most commonly occurring malignancies worldwide. The incidence of head and neck squamous cell carcinoma (HNSCC) is estimated at 500,000 per year in the United States (1). Changes have been made regarding the therapeutic strategies applied for such patients, including different surgical approaches, radiotherapy and chemotherapy either in alone or in various combinations; however, despite this multi-modal treatment strategy, survival rates have not improved significantly over the past several decades, with an overall 5-year survival rate of 40-50% (2).There is increasing evidence that tumor growth is not only determined by malignant cancer cells, but also by the tumor stroma (3). The tumor stroma is partially composed of fibroblasts and the connective tissue they produce. Physiologically, due to their close proximity, continuous crosstalk between the stroma and epithelia controls tissue differentiation (4). In the pathology of a tissue wound, the stroma takes on the role of repair, while paracrine signaling alters epithelial proliferation and differentiation (4,5). As cancer functionally resembles a chronic wound, these mechanisms of wound repair are useful to examine from an oncological point of view. In this regard, fibroblasts, which are the primary component of tumor stroma, have been shown to be prominent modifiers of cancer progression (6). It has also become increasingly clear that there are different subpopulations of fibroblasts, as a result of their continuous interaction with epithelial or cancer cells. One of these subpopulations, termed cancer-associated fibroblasts (CAFs), has been shown to be an important promoter of t...

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Enhancement of head and neck squamous cell carcinoma proliferation, invasion, and metastasis by tumor‐associated fibroblasts in preclinical models

et al. 2013

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Background Head and neck squamous cell carcinoma (HNSCC) has had little improvement in mortality rates in decades. A clearer understanding of the HNSCC tumor microenvironment will aid in finding more effective targeted therapies for this disease. Tumor associated fibroblasts (TAFs) are the largest stromal cellular components of the tumor microenvironment in HNSCC. Methods We isolated TAFs from clinical HNSCC cases and propagated in vitro. The effects of TAF secreted paracrine factors on in vitro HNSCC migration, invasion and proliferation was assessed. The effect of TAFs on HNSCC growth and metastases was determined in an orthotopic floor of mouth tumor model. Results TAF conditioned media increased HNSCC cell migration, invasion and proliferation. TAFs increased HNSCC tumor growth and metastases in vivo. Conclusions TAFs play a major role in increasing tumor growth and metastasis in HNSCC. Targeting the tumor stroma may be important to reduce the rate of HNSCC metastasis.

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Tumor–stroma interactions influence cytokine expression and matrix metalloproteinase activities in paired primary and metastatic head and neck cancer cells

Cited by 32 publications

References 26 publications

Possible mechanisms of retinal function recovery with the use of cell therapy with bone marrow-derived stem cells

Possible mechanisms of retinal function recovery with the use of cell therapy with bone marrow-derived stem cells

Differences in tumor stroma derived from irradiated versus non-irradiated fibroblasts in a co-culture model with head and neck squamous cell carcinoma

Enhancement of head and neck squamous cell carcinoma proliferation, invasion, and metastasis by tumor‐associated fibroblasts in preclinical models

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