Tumor-promoting effects of cannabinoid receptor type 1 in human melanoma cells

Carpi, Sara; Fogli, Stefano; Polini, Beatrice; Montagnani, Valentina; Podestà, Adriano; Breschi, Maria Cristina; Romanini, Antonella; Stecca, Barbara; Nieri, Paola

doi:10.1016/j.tiv.2017.01.018

Cited by 28 publications

(26 citation statements)

References 39 publications

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“…Such compounds may allow for avoiding CB1 activation-related adverse effects. However, there is a report on tumor-progressive roles of CB1 agonist in melanoma cells [271].…”

Section: Cancermentioning

confidence: 99%

A Guide to Targeting the Endocannabinoid System in Drug Design

Stasiulewicz

Znajdek

Grudzień

et al. 2020

IJMS

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The endocannabinoid system (ECS) is one of the most crucial systems in the human organism, exhibiting multi-purpose regulatory character. It is engaged in a vast array of physiological processes, including nociception, mood regulation, cognitive functions, neurogenesis and neuroprotection, appetite, lipid metabolism, as well as cell growth and proliferation. Thus, ECS proteins, including cannabinoid receptors and their endogenous ligands’ synthesizing and degrading enzymes, are promising therapeutic targets. Their modulation has been employed in or extensively studied as a treatment of multiple diseases. However, due to a complex nature of ECS and its crosstalk with other biological systems, the development of novel drugs turned out to be a challenging task. In this review, we summarize potential therapeutic applications for ECS-targeting drugs, especially focusing on promising synthetic compounds and preclinical studies. We put emphasis on modulation of specific proteins of ECS in different pathophysiological areas. In addition, we stress possible difficulties and risks and highlight proposed solutions. By presenting this review, we point out information pivotal in the spotlight of ECS-targeting drug design, as well as provide an overview of the current state of knowledge on ECS-related pharmacodynamics and show possible directions for needed research.

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“…Such compounds may allow for avoiding CB1 activation-related adverse effects. However, there is a report on tumor-progressive roles of CB1 agonist in melanoma cells [271].…”

Section: Cancermentioning

confidence: 99%

A Guide to Targeting the Endocannabinoid System in Drug Design

Stasiulewicz

Znajdek

Grudzień

et al. 2020

IJMS

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“…The cDNA obtained was diluted 1:3 in RNase-free water from adipocytes, while the exosome-cDNA was used without dilution. The qPCR experiments were performed by miScript SYBR-Green PCR kit (Qiagen), as previously reported [52]. Signals were detected on the MiniOpticon CFX 48 real-time PCR Detection System (Bio-Rad, Hercules, CA, USA).…”

Section: Evaluation Of Mirna Expressionmentioning

confidence: 99%

The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation

et al. 2019

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Inflammation of the adipose tissue plays an important role in the development of several chronic diseases associated with obesity. Polyphenols of extra virgin olive oil (EVOO), such as the secoiridoids oleocanthal (OC) and oleacein (OA), have many nutraceutical proprieties. However, their roles in obesity-associated adipocyte inflammation, the NF-κB pathway and related sub-networks have not been fully elucidated. Here, we investigated impact of OC and OA on the activation of NF-κB and the expression of molecules associated with inflammatory and dysmetabolic responses. To this aim, fully differentiated Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were pre-treated with OC or OA before stimulation with TNF-α. EVOO polyphenols significantly reduced the expression of genes implicated in adipocyte inflammation (IL-1β, COX-2), angiogenesis (VEGF/KDR, MMP-2), oxidative stress (NADPH oxidase), antioxidant enzymes (SOD and GPX), leukocytes chemotaxis and infiltration (MCP-1, CXCL-10, MCS-F), and improved the expression of the anti-inflammatory/metabolic effector PPARγ. Accordingly, miR-155-5p, miR-34a-5p and let-7c-5p, tightly connected with the NF-κB pathway, were deregulated by TNF-α in both cells and exosomes. The miRNA modulation and NF-κB activation by TNF-α was significantly counteracted by EVOO polyphenols. Computational studies suggested a potential direct interaction between OC and NF-κB at the basis of its activity. This study demonstrates that OC and OA counteract adipocyte inflammation attenuating NF-κB activation. Therefore, these compounds could be novel dietary tools for the prevention of inflammatory diseases associated with obesity.

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“…THC has been shown to amplify the expression of KSHV GPCR, unleashing the proliferation of endothelial cells and inducing the apparition of Kaposi sarcoma in vitro, on human cell lines; these effects seem to be dose-dependent [196]. Endocannabinoids may be involved in the neural metastasis of melanoma via the CB1 receptor by similarly stimulating tumor cell migration as neuron migration, as demonstrated in vitro on A375 and 501 Mel melanoma cell lines [176,204].…”

Section: Adverse Effects Of Cannabinoidsmentioning

confidence: 95%

“…Conversely, the CB1 receptor was identified as having tumor-promoting effects in knockdown models of melanoma, as the CB1-silenced group demonstrated inhibition of ERK and Akt phosphorylation and cell cycle arrest; these findings lead to the hypothesis that CB1 expression alters communication and feedback loops in the endocannabinoid system, mediating the inhibition of migration and proliferation of melanoma cells in vitro [176]. Furthermore, this controversial pro-tumoral effect observed in vitro may stand to prove that the anti-tumoral effects of cannabinoids cited in various in vivo studies rely on impacting the inflammatory milieu, a factor which is improperly represented in in vitro studies [11,172].…”

Section: Melanomamentioning

confidence: 99%

Cannabinoids in the Pathophysiology of Skin Inflammation

et al. 2020

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Cannabinoids are increasingly-used substances in the treatment of chronic pain, some neuropsychiatric disorders and more recently, skin disorders with an inflammatory component. However, various studies cite conflicting results concerning the cellular mechanisms involved, while others suggest that cannabinoids may even exert pro-inflammatory behaviors. This paper aims to detail and clarify the complex workings of cannabinoids in the molecular setting of the main dermatological inflammatory diseases, and their interactions with other substances with emerging applications in the treatment of these conditions. Also, the potential role of cannabinoids as antitumoral drugs is explored in relation to the inflammatory component of skin cancer. In vivo and in vitro studies that employed either phyto-, endo-, or synthetic cannabinoids were considered in this paper. Cannabinoids are regarded with growing interest as eligible drugs in the treatment of skin inflammatory conditions, with potential anticancer effects, and the readiness in monitoring of effects and the facility of topical application may contribute to the growing support of the use of these substances. Despite the promising early results, further controlled human studies are required to establish the definitive role of these products in the pathophysiology of skin inflammation and their usefulness in the clinical setting.

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Tumor-promoting effects of cannabinoid receptor type 1 in human melanoma cells

Cited by 28 publications

References 39 publications

A Guide to Targeting the Endocannabinoid System in Drug Design

A Guide to Targeting the Endocannabinoid System in Drug Design

The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation

Cannabinoids in the Pathophysiology of Skin Inflammation

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