Tumor prevalence and biomarkers of genotoxicity in brown bullhead (Ameiurus nebulosus) in Chesapeake Bay tributaries

Pinkney, Alfred E.; Harshbarger, John C.; Karouna‐Renier, Natalie K.; Jenko, Kathryn; Balk, Lennart; Skarphéðinsdóttir, Halldóra; Liewenborg, Birgitta; Rutter, Michael A.

doi:10.1016/j.scitotenv.2011.09.035

Cited by 18 publications

(26 citation statements)

References 54 publications

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“…In fish, water or food exposure to PAHs and PHAHs has been reported to result in numerous biological effects such as weight loss, immunotoxicity, hepatotoxicity, cardiotoxicity, edema formation, genotoxicity and tumor promotion (e.g. Tanguay et al 2005;Reynaud and Deschaux 2006;Pinkney et al 2011;Nahrgang et al 2010;Wills et al 2010;Hicken et al 2011). Such compounds are known to affect early life stages development, that is, malformations of embryo and larvae (Barron et al 2004;Sundberg et al 2006, Billiard et al 2008, and cause alteration of endocrine system function and reproduction (Monosson 1999(Monosson /2000.…”

Section: Introductionmentioning

confidence: 99%

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Durieux

Connon

Werner

et al. 2012

Fish Physiol Biochem

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The striped bass (Morone saxatilis) supports a valuable recreational fishery and is among the most important piscivorous fish of the San Francisco Estuary. This species has suffered a significant decline in numbers over the past decades, and there is indication that contaminants are important contributors. Polycyclic aromatic hydrocarbons (PAHs) and polyhalogenated aromatic hydrocarbons (PHAHs) including PCBs and dioxins are widespread in the estuary, they typically bioaccumulate through trophic levels, reaching highest levels in top predators and are known to affect the fish health and development. The aim of this study was to investigate the dynamics of cytochrome P4501A (Cyp1a) induction simultaneously at different levels of biological organization (RNA transcription and protein synthesis) as a biomarker of exposure to PAHs and PHAHs. We utilized β-naphthoflavone (BNF) as a model PAH to induce Cyp1a responses in juvenile striped bass in both dose-response and time-response assessments and determined Cyp1a mRNA and protein levels. Significant responses were measured in both systems at 10 mg ΒΝF kg⁻¹, a concentration used for time-response studies. Messenger RNA levels peaked at 6 h post-injection, while protein levels increased progressively with time, significantly peaking at 96 h post-injection; both remaining elevated throughout the duration of the test (8 days). Our data suggest that rapid induction of gene transcription following exposure and subsequent cumulative protein synthesis could provide a useful means of identifying temporal variants in exposure to Cyp1a inducers in Morone saxatilis. The potential application of this combined Cyp1a gene and protein biomarker in this species for field studies is discussed.

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Section: Introductionmentioning

confidence: 99%

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Durieux

Connon

Werner

et al. 2012

Fish Physiol Biochem

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“…It is important to note that neoplasia in the brown bullhead is not exclusive to the Great Lakes basin and has been reported elsewhere in the United States (Bowser et al, 1991;Brown et al, 1973;Brown and Sinclair, 1977;Kim et al, 1989;Osburn, 1925;Pinkney et al, 2001Pinkney et al, , 2004aPinkney et al, , 2004bSpitsbergen and Wolfe, 1995;Steyermark et al, 1999). The brown bullhead is a scaleless, bottomdwelling catfish that inhabits slow moving fresh and brackish waters with a sand or mud bottom and abundant aquatic vegetation (Sinnott and Ringler, 1987), and is considered to have a limited home range (Millard et al, 2009;Obert, 1994;Sakaris et al, 2005).…”

Section: Neoplasia In Brown Bullheadmentioning

confidence: 79%

A historical perspective on the “fish tumors or other deformities” beneficial use impairment at Great Lakes Areas of Concern

Rafferty

Blazer

Pinkney

et al. 2009

Journal of Great Lakes Research

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“…Genotoxic pollutants may have mutagenic and (or) carcinogenic properties, that affect a cell's genetic material, causing mutations and in some cases contributing to the tumor development and neoplasia (Pinkney et al, 2011;Williams, 2012). A variety of methods have been used with fishes to detect exposure to genotoxic compounds.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results

Blazer¹,

Mazik²,

Iwanowicz³

et al. 2014

Open-File Report

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For more information on the USGS-the Federal source for science about the Earth, its natural and living resources, natural hazards, and the environment-visit http://www.usgs.gov or call 1-888-ASK-USGS (1-888-275-8747) For an overview of USGS information products, including maps, imagery, and publications, visit http: //www.usgs.gov/pubprod To order this and other USGS information products, visit http://store.usgs.gov Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.Although this information product, for the most part, is in the public domain, it also may contain copyrighted materials as noted in the text. Permission to reproduce copyrighted items must be secured from the copyright owner.This draft manuscript is distributed solely for purposes of scientific peer review. Its content is deliberative and predecisional, so it must not be disclosed or released by reviewers. Because the manuscript has not yet been approved for publication by the U.S. Geological Survey, it does not represent any official USGS finding or policy. AbstractDuring fall 2010 and spring 2011, a total of 119 brown bullhead (Ameiurus nebulosus), 136 white sucker (Catostomus commersoni), 73 smallmouth bass (Micropterus dolomieu), and 59 largemouth bass (M. salmoides) were collected from seven Great Lakes Basin Areas of Concern and one Reference Site. Comprehensive fish health assessments were conducted in order to document potential adverse effects from exposure to complex chemical mixtures. Fish were necropsied on site, blood samples obtained, pieces of liver, spleen, kidney, gill and any abnormalities placed in fixative for histopathology. Liver samples were saved for gene expression analysis and otoliths were removed for aging. A suite of fish health indicators was developed and implemented for site comparisons and to document seasonal effects and species differences in response to environmental conditions. Organism level (grossly visible lesions, condition factor), tissue level (microscopic pathology, organosomatic indices, micronuclei, and other nuclear abnormalities), plasma factors (reproductive steroid hormones, vitellogenin), and molecular (gene expression) indicators were included. This report describes the methods and preliminary results.

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Tumor prevalence and biomarkers of genotoxicity in brown bullhead (Ameiurus nebulosus) in Chesapeake Bay tributaries

Cited by 18 publications

References 54 publications

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

A historical perspective on the “fish tumors or other deformities” beneficial use impairment at Great Lakes Areas of Concern

Monitoring of wild fish health at selected sites in the Great Lakes Basin: methods and preliminary results

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