Tumor neoangiogenesis detection by confocal laser endomicroscopy and anti-CD105 antibody: Pilot study

Abstract

AIM:To evaluate neoangiogenesis in patients with colon cancer by two fluorescently labeled antibodies on fresh biopsy samples imaged with confocal laser endomicroscopy (CLE). . When using the anti-CD31 antibody, the average diameter of vessels in the normal colon tissue was 7.67 ± 0.5 μm and the vessel density was 3191.60 ± 387.8 vessels/mm 3 , while in the tumors we obtained an average diameter of 10.88 ± 0.8 μm and a vessel density of 4707.30 ± 448.85 vessels/mm 3 . Thus, there were more vessels stained with CD31 than CD105 (P < 0.05). The average vessel diameter was similar for both CD31 and CD105 staining. A qualitative comparison between CLE vs immunohistochemistry lead to similar results. CONCLUSION:Specific imaging and quantification of tumor microvessels are feasible in human rectal cancer using CLE examination and CD105 immunostaining of fresh tissue samples. Core tip: We evaluated CD105 expression from fresh tissue samples of human rectal adenocarcinoma, using confocal laser endomicroscopy (CLE). While vessels marked with fluorescent CD31 were visible in both normal and malignant tissue, CD105 was predominantly expressed in tumor lesions, having reduced affinity for normal rectal mucosa. Our data showed that CLE using CD105 antibody for tumor vascular network imaging is feasible and that CD105 represents a more specific marker for rectal cancer neoangiogenesis than panendothelial markers. To our knowledge, this is the first study to report the use of fluorescently-labeled CD105 antibody in conjunction with CLE in patients with rectal tumor.

Probe-based confocal laser endomicroscopy for in vivo evaluation of the tumor vasculature in gastric and rectal carcinomas

“…The present investigation is the first to take into account the analysis of tumor vasculature by pCLE in a considerable number of rectal and gastric cancers. Two recent studies showed that the use of confocal laser endomicroscopy in colorectal cancers was suitable for measuring more vascular parameters than conventional immunohistochemistry alone, and for detecting tumor neoangiogenesis with fluorescently labeled antibodies 31 , 32 . The approach used by the authors was completely different: the pCLE analysis was not performed during the endoscopic examination but on fresh tumor biopsies.…”

Küçük hücreli dışı akciğer kanserlerinde endoglin, CD31 ve VEGFR2 ile saptanan mikrodamar yoğunluğu ve sağkalıma etkisi

“…Endoglin, tümör içinde veya çevresinde yüksek hassasiyete sahip küçük damarları boyarken, neoplastik olmayan dokudaki kan damarlarını boyamadığı veya sadece zayıf boyadığı ifade edilmiştir. Buna karşılık CD31'in ise tüm kan damarlarını ayrım gözetmeden boyadığı görülmüştür [25,26]. Glioblastomlu hastalar üzerinde yapılan çalışmada da benzer sonuca ulaşılmış, endoglinin glioblastomadaki anjiyogenezin değerlendirilmesinde CD31'e göre daha spesifik ve hassas bir belirteç olduğu gösterilmiştir [27].…”

Introductory Chapter: Endoscopy-Novel Techniques and Recent Advancements

“…The most used dye is fluorescein which has a safety profile, and has the ability of highlighting the vessels. However, the direction seems to be toward individualized situations whereas specific antibodies such as CD 31, CD105, and EGFR [24,25] might be more useful for tissue architecture description. CLE is considered a valuable tool with great potential that may overcome some of the disadvantages of classic histology such as time waiting or sampling bias, thus facilitating live diagnosis and treatment decisions.…”