Tumor necrosis factor-α and interleukin-6 gene polymorphism association with susceptibility to celiac disease in Italian patients

Abstract

ABSTRACT. The aim of this research was to study polymorphisms in the genes encoding cytokines interleukin-6 (IL-6) and tumor necrosis factoralpha (TNF-α) in patients with celiac disease (CD) antigens DQ2 (DQ2-positive) or DQ8 (DQ8-positive). We compared the results with healthy controls to determine whether any of the polymorphisms have a role in susceptibility to CD. 16343-16352 (2015) included in the study. Analysis of single nucleotide polymorphisms (SNPs) was carried out using the polymerase chain reaction-restriction fragment length polymorphism method. Significant differences for the TNF-α (-308 G>A) polymorphism were observed when we compared the flowing groups: DQ2-positive with controls [odds ratio (OR) = 0.45, P = 0.0002]; DQ8-positive with controls (OR = 3.55, P < 0.0001); and DQ2-positive with DQ8-positive (OR = 0.12, P < 0.0001). We did not observe a statistically significant association between IL-6 (-174 G>C) polymorphism and CD (P > 0.05). Our results suggest that TNF-α (-308 G>A) polymorphism may play a role in susceptibility to CD in Italian patients.

TNF-α -308 G > A (rs1800629) Polymorphism is Associated with Celiac Disease: A Meta-analysis of 11 Case-Control Studies

“…Most of the evidences suggest that the endogenous production of TNF-α may be influenced by TNF-α promoter polymorphisms, thereby affecting messenger RNA (mRNA) and protein expression levels26. In view of facts about the significant role of TNF-α gene in modulation of acute inflammation and host innate immunity, various studies have been conducted to analyze the relationship between TNF-α -308 G > A gene polymorphism and the development of CD, but the results from different published studies were contradictory and inconclusive1415161718192021222324. In order to improve the statistical power, we performed the present meta-analysis to assess the said association of TNF-α -308 G > A polymorphism and CD risk from eleven case-control studies, as combining of the data from different studies has an advantage of reducing the random error27.…”

“…Keeping aforesaid information in view, to date a number of case control and cohort studies have been performed to investigate the association of TNF-α -308 G > A gene polymorphism and CD susceptibility1415161718192021222324. However, the results are inconsistent and controversial because of small sample size of individual study and possible selective bias.…”

Interleukin-6 and interleukin-17 gene polymorphism association with celiac disease in children

“…Dema et al (7) determined that IL-6 (-174G/C) polymorphism increased the risk of CD in girls. However, another study determined no relation between this polymorphism and CD (2). IL-6 (-572G/C) (rs1800796) is found adjacent to the IL-6 5' promoter region (8).…”

“…Genetic polymorphisms have been reported to contribute to CD sensitivity by altering the levels and functions of cytokines (2). This study aimed to evaluate IL-6 (-572G/C) (rs1800796) and IL-17 (-197A/G) (rs2275913) gene polymorphisms in children with CD.…”

Interleukin-6 (IL-6) -174G/C genomic polymorphism contribution to the risk of coronary artery disease in a Chinese population

“…The IL-6 gene, located at chromosome 7p21-24, is composed of 4 introns and 5 exons. Since single nucleotide polymorphisms of IL-6 gene promoter may affect the expression and secretion of IL-6, and subsequently the altered circulating levels might result in relevant biological responses, and the IL-6 polymorphism has been regarded as a crucial modulator in pathogenesis of various diseases, including Alzheimer's disease, type 2 diabetes, celiac disease, chronic obstructive pulmonary disease and osteoarthritis (de Albuquerque Maranhão et al, 2015;Fernandes et al, 2015;Xie et al, 2015;Buraczynska et al, 2016;Ramos Dos Santos et al, 2016). Although several studies have shown that IL-6 polymorphisms could be also involved in the development of CAD, the conclusions were not consistent Liu et al, 2015;Wang et al, 2015;Yang et al, 2015).…”