Tumor necrosis factor alpha promoter polymorphism studies in pregnant women

Khan, Imran Ali; Kamineni, Vasundhara; Poornima, Subhadra; Jahan, Parveen; Hasan, Qurratulain; Rao, Pragna

doi:10.1016/j.jrhm.2015.01.001

Cited by 13 publications

(8 citation statements)

References 35 publications

(24 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many other reports with other marker systems showed lower percent polymorphisms relative to our findings in this study. For instance, RAPD and microsatellite markers revealed 71.2% [ 52 ], 90.0% [ 55 ], 55.5% [ 56 ], 46.5% [ 57 , 58 ], 64.5% [ 59 ] and 58.44% polymorphism [ 30 ]. The percentage polymorphism obtained from this present study is quite high indicating the higher informative nature of the markers used (ISSR and SCoT).…”

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers

et al. 2017

View full text Add to dashboard Cite

Background: Assessment of genetic diversity of Vigna unguiculata (L.) Walp (cowpea) accessions using informative molecular markers is imperative for their genetic improvement and conservation. Use of efficacious molecular markers to obtain the required knowledge of the genetic diversity within the local and regional germplasm collections can enhance the overall effectiveness of cowpea improvement programs, hence, the comparative assessment of Inter-simple sequence repeat (ISSR) and Start codon targeted (SCoT) markers in genetic diversity of V. unguiculata accessions from different regions in Nigeria. Comparative analysis of the genetic diversity of eighteen accessions from different locations in Nigeria was investigated using ISSR and SCoT markers. DNA extraction was done using Zymogen Kit according to its manufacturer's instructions followed by amplifications with ISSR and SCoT and agarose gel electrophoresis. The reproducible bands were scored for analyses of dendrograms, principal component analysis, genetic diversity, allele frequency, polymorphic information content, and population structure.

show abstract

Section: Discussionmentioning

confidence: 99%

Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Genomic DNA was extracted from peripheral leucocytes using the salting-out technique, routinely used in our laboratory, as previously described. 20 The DNA samples were stored at −80 °C until processed. Genotyping for KCNQ1 (rs2283228) and KCNJ11 (rs5210) variants was carried out via PCR-RFLP in a 25 μL reaction (Bangalore Genei kit; Bangalore Genei Pvt.…”

Section: Methodsmentioning

confidence: 99%

Correlation between KCNQ1 and KCNJ11 gene polymorphisms and type 2 and post-transplant diabetes mellitus in the Asian Indian population

et al. 2015

Self Cite

View full text Add to dashboard Cite

Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) share a common pathophysiology. However, diabetes mellitus is a complex disease, and T2DM and PTDM have different etiologies. T2DM is a metabolic disorder, characterized by persistent hyperglycemia, whereas PTDM is a condition of abnormal glucose tolerance, with variable onset after organ transplant. The KCNQ1 and KCNJ11 gene encode potassium channels, which mediate insulin secretion from pancreatic β-cells, and KCN gene mutations are correlated with the development of diabetes. However, no studies have been carried out to establish an association between KCNQ1 and KCNJ11 gene polymorphisms and T2DM and PTDM. Therefore, our study was aimed at the identification of the role of KCNQ1 and KCNJ11 gene polymorphisms associated with T2DM and the risk of developing PTDM in the Asian Indian population. We have carried out a case–control study including 250 patients with T2DM, 250 control subjects, 42 patients with PTDM and 98 subjects with non-PTDM. PCR-RFLP analysis was carried out following the isolation of genomic DNA from EDTA-blood samples. The results of the present study reveal that two single nucleotide polymorphisms (rs2283228 and rs5210, of the KCNQ1 and KCNJ11 genes, respectively) are associated with both T2DM and PTDM. The results of our study suggest a role of KCNQ1 and KCNJ11 gene variants in the increased risk of T2DM and PTDM in the Asian Indian population.

show abstract

“…DNA was routinely purified at Kamineni Hospitals using a salting out procedure (12,13). The region 173 bp upstream of the GCK coding region was genotyped with polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis.…”

Section: Mutational Analysismentioning

confidence: 99%

Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Khan

Vattam

Jahan

et al. 2015

IRDR

Self Cite

View full text Add to dashboard Cite

Tumor necrosis factor alpha promoter polymorphism studies in pregnant women

Cited by 13 publications

References 35 publications

Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers

Assessment of genetic diversity in Vigna unguiculata L. (Walp) accessions using inter-simple sequence repeat (ISSR) and start codon targeted (SCoT) polymorphic markers

Correlation between KCNQ1 and KCNJ11 gene polymorphisms and type 2 and post-transplant diabetes mellitus in the Asian Indian population

Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

Contact Info

Product

Resources

About