Tumor necrosis factor-alpha modifies adhesion properties of rat islet B cells.

Cirulli, Vincenzo; Halban, Philippe A.; Rouiller, D.

doi:10.1172/jci116403

Cited by 27 publications

(25 citation statements)

References 51 publications

(69 reference statements)

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“…Exposure to tumor necrosis factor or antibodies against E-cadherin results in an abnormal islet architecture and a decreased cell adhesion, respectively, which are associated with a decrease in insulin release (Cirulli et al 1993, Yamagata et al 2002. These findings reinforce the idea that CAM-mediated cell-cell contact is crucial for a proper secretory response by the endocrine pancreas.…”

Section: Discussionsupporting

confidence: 67%

Upregulation of the expression of tight and adherens junction-associated proteins during maturation of neonatal pancreatic islets in vitro

et al. 2004

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Cell-cell contacts mediated by intercellular junctions are crucial for proper insulin secretion in the endocrine pancreas. The biochemical composition of the intercellular junctions in this organ and the role of junctional proteins in endocrine pancreatic dysfunctions are still unclear. In this study, we investigated the expression and cellular location of junctional and cytoskeletal proteins in cultured neonatal rat pancreatic islets. Neonatal B-cells had an impaired insulin secretion compared to adult cells. Cultured neonatal islets showed a time-dependent increase in the glucose-induced secretory response. The maturation of B-cells in vitro was accompanied by upregulation of the expression of some junctional proteins in islet cells. Neonatal islets cultured for only 24 h showed a low expression and a diffuse cytoplasmic location of the tight junctional proteins occludin and ZO-1 and of the adherens junctional proteins alpha- and beta-catenins, as demonstrated by immunoblotting and immunocytochemistry. Culturing islets for up to 8 days significantly increased the cell expression of these junctional proteins but not of the cytoskeletal proteins vinculin and alpha-actinin. A translocation of ZO-1 and catenins to the cell-cell contact region, as well as a higher association of F-actin with the intercellular junction, were also observed in neonatal islets following prolonged culturing. ZO-1 and beta-catenin were immunolocated in the endocrine pancreas of adult rats indicating that these junctional proteins are also expressed in this organ in situ. In conclusion, endocrine pancreatic cells express several junctional proteins that are upregulated following differentiation of the endocrine pancreas in vitro.

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Section: Discussionsupporting

confidence: 67%

Upregulation of the expression of tight and adherens junction-associated proteins during maturation of neonatal pancreatic islets in vitro

et al. 2004

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“…These cell aggregates showed within one to three weeks of in vitro culture a cellular architecture of endocrine core and outer CK-19 positive cells similar to that of encapsulated islets (Fig. 3) and reassociated islets (31)(32)(33), suggesting that the proliferated cells had retained the ability to form islet-like structures. Furthermore, by controlling the cell load per capsule, we were able to generate aggregates roughly the size of average human islets (Fig.…”

Section: Discussionmentioning

confidence: 86%

Generation of Functional Islet-Like Clusters After Monolayer Culture and Intracapsular Aggregation of Adult Human Pancreatic Islet Tissue

Tsang

Zheng²,

Wang³

et al. 2007

Transplantation

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Collectively, these data indicate that adult human pancreatic islet cells can be expanded by three serial passages while maintaining their endocrine properties and can yield functional islet-like cell clusters through intracapsular aggregation that reverse hyperglycemia in diabetic mice. This culture and aggregation process could serve as a platform for proliferation and differentiation studies of endocrine lineage cells.

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“…In animal models of diabetes, the well-defined islet architecture is disturbed [6,7] so that glucagon-and somatostatincontaining cells are intermingled with beta cells. The same altered cellular organisation was reproduced in vitro by exposing islet cells to cytokines known to be secreted by leucocytes that infiltrate islets during diabetes onset [8].…”

Section: Introductionmentioning

confidence: 87%

Insulin secretion from human beta cells is heterogeneous and dependent on cell-to-cell contacts

Wojtusciszyn

Armanet²,

Morel³

et al. 2008

Diabetologia

126

102

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Aims/hypothesis We assessed the heterogeneity of insulin secretion from human isolated beta cells and its regulation by cell-to-cell contacts. Methods Insulin secretion from single and paired cells was assessed by a reverse haemolytic plaque assay. The percentage of plaque-forming cells, the mean plaque area and the total plaque development were evaluated after 1 h of stimulation with different secretagogues. Results Not all beta cells were surrounded by a haemolytic plaque under all conditions tested. A small fraction of the beta cell population (20%) secreted more than 90% and 70% of total insulin at 2.2 and 22.2 mmol/l glucose, respectively. Plaque-forming cells, mean plaque area and total plaque development were increased at 12.2 and 22.2 compared with 2.2 mmol/l glucose. Insulin secretion of single beta cells was similar at 12.2 and 22.2 mmol/l glucose. Insulin secretion of beta cell pairs was increased compared with that of single beta cells and was higher at 22.2 than at 12.2 mmol/l glucose. Insulin secretion of beta cells in contact with alpha cells was also increased compared with single beta cells, but was similar at 22.2 compared with 12.2 mmol/l glucose. Delta and other non-beta cells did not increase insulin secretion of contacting beta cells compared with that of single beta cells. Differences in insulin secretion between 22.2 and 12.2 mmol/l glucose were observed in murine but not in human islets. Conclusions/interpretation Human beta cells are highly heterogeneous in terms of insulin secretion so that a small fraction of beta cells contributes to the majority of insulin secreted. Homologous and heterologous intercellular contacts have a significant impact on insulin secretion and this could be related to the particular architecture of human islets.

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Tumor necrosis factor-alpha modifies adhesion properties of rat islet B cells.

Cited by 27 publications

References 51 publications

Upregulation of the expression of tight and adherens junction-associated proteins during maturation of neonatal pancreatic islets in vitro

Upregulation of the expression of tight and adherens junction-associated proteins during maturation of neonatal pancreatic islets in vitro

Generation of Functional Islet-Like Clusters After Monolayer Culture and Intracapsular Aggregation of Adult Human Pancreatic Islet Tissue

Insulin secretion from human beta cells is heterogeneous and dependent on cell-to-cell contacts

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