Tumor Necrosis Factor-alpha in Gingival Crevicular Fluid as a Diagnostic Marker for Periodontal Diseases: A Systematic Review

Madureira, Davidson Fróis; Lima, Izabella Lucas de Abreu; Costa, Giselle Cabral; Lages, Elizabeth Maria Bastos; Martins, Carolina Castro; Silva, Tarcı́lia Aparecida

doi:10.1016/j.jebdp.2018.04.001

Cited by 34 publications

(26 citation statements)

References 51 publications

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“…Several polymorphisms in the promoter region of TNF-α have been identified, and the 308G/A and 863C/A polymorphisms may contribute to the susceptibility of periodontitis according to a meta-analysis. 22 The TNF level was also shown to be elevated in the GCF 122 and serum 123 of chronic periodontitis patients, while no decrease in TNF was observed after NSPT. These results can be explained by the participation of TNF in bone metabolism, and studies have shown that TNF upregulates RANKL expression in gingival epithelial cells, 124,125 T cells and osteoblasts.…”

Section: Pro-inflammatory Cytokines Involved In Periodontitismentioning

confidence: 91%

The cytokine network involved in the host immune response to periodontitis

2019

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Periodontitis is an inflammatory disease involving the destruction of both soft and hard tissue in the periodontal region. Although dysbiosis of the local microbial community initiates local inflammation, over-activation of the host immune response directly activates osteoclastic activity and alveolar bone loss. Many studies have reported on the cytokine network involved in periodontitis and its crucial and pleiotropic effect on the recruitment of specific immunocytes, control of pathobionts and induction or suppression of osteoclastic activity. Nonetheless, particularities in the stimulation of pathogens in the oral cavity that lead to the specific and complex periodontal cytokine network are far from clarified. Thus, in this review, we begin with an up-to-date aetiological hypothesis of periodontal disease and summarize the roles of cytokines in the host immune response. In addition, we also summarize the latest cytokine-related therapeutic measures for periodontal disease.

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Section: Pro-inflammatory Cytokines Involved In Periodontitismentioning

confidence: 91%

The cytokine network involved in the host immune response to periodontitis

2019

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“…Moreover, in order to mimic inflammatory conditions in vitro, cells were treated with human recombinant tumor necrosis factor (TNF)α (Biomol, Hamburg, Germany), which is one of the key inflammatory mediators in the pathogenesis of the periodontal diseases. TNFα was applied at a concentration of 1 ng/ml, which is in the range of levels usually found in the gingival crevicular fluid (GCF) of periodontally diseased patients and which has been used by other investigators [22,23]. Untreated cells served as a control.…”

Section: Methodsmentioning

confidence: 99%

Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells

Nokhbehsaim

Nogueira

Nietzsche

et al. 2020

Mediators of Inflammation

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Periodontitis is a prevalent chronic inflammatory disease triggered by a synergistic and dysbiotic microbiota present in the oral biofilm. This in vitro study is aimed at evaluating the regulation of cyclooxygenase (COX)2 expression and production by the periodontopathogen Filifactor alocis in human gingival fibroblastic (HGF-1) and monocytic (THP-1) cells and also at investigating the underlying cellular pathway mechanisms. HGF-1 and THP-1 cells were exposed either to F. alocis or to the proinflammatory cytokine tumor necrosis factor alpha (TNFα) for 1 and 2 d to examine the COX2 expression by qPCR. COX2 protein levels were evaluated by ELISA in F. alocis-stimulated cells. Both types of cells were also stimulated with a blocking toll-like receptor (TLR)2 antibody or specific inhibitors against MAPKs. F. alocis significantly (p<0.05) increased COX2 at both transcriptional and protein levels in both HGF-1 and THP-1 cells. Moreover, the stimulatory effect of F. alocis on COX2 was more pronounced in HGF-1 cells in comparison to THP-1 cells. F. alocis upregulated the COX2 expression in a dose-dependent manner in both type cells at 1 d. TNFα also significantly (p<0.05) increased the COX2 expression in both cells. After preincubation of HGF-1 and THP-1 cells either with a neutralizing anti-TLR2 antibody or with specific MAPK inhibitors, the F. alocis-upregulated COX2 expression was significantly (p<0.05) suppressed at 1 d. Our in vitro study provides original evidence that F. alocis stimulates COX2 production in fibroblastic and monocytic cells through TLR2 and MAPK mechanisms, suggesting a role of this periodontopathogen in the etiopathogenesis of periodontitis.

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“…TNF-α is considered a key cytokine involved with the innate response against the periodontopathogenic bacteria. Although no correlation could be found between the levels of TNF-α in GCF in different degrees of periodontitis, this molecule showed a strong correlation with the severity of periodontal destruction, and it could be used to compare the various stages of periodontal disease [39]. In our study, we have proved the presence of TNF-α in GCF of patients with periodontitis and PISF obtained from individuals without any clinical symptoms of developing mucositis or peri-implantitis.…”

Section: Discussionmentioning

confidence: 59%

Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

Aleksandrowicz

Brzezińska‐Błaszczyk

Kozłowska

et al. 2020

Preprint

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Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis.Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis.Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

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Tumor Necrosis Factor-alpha in Gingival Crevicular Fluid as a Diagnostic Marker for Periodontal Diseases: A Systematic Review

Cited by 34 publications

References 51 publications

The cytokine network involved in the host immune response to periodontitis

The cytokine network involved in the host immune response to periodontitis

Regulation of Cyclooxygenase 2 by Filifactor alocis in Fibroblastic and Monocytic Cells

Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

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