1996
DOI: 10.1053/jhep.1996.v23.pm0008591843
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Tumor necrosis factor alpha gene expression and the response to interferon in chronic hepatitis

Abstract: Tumor necrosis factor alpha (TNF-alpha) is a cytokine with pleiotropic properties that is induced in a variety of pathological situations including viral infections. In this work, we analyzed the expression of TNF-alpha gene in patients with chronic hepatitis C. Serum TNF-alpha levels were found to be elevated in all chronic hepatitis C patients including those cases presenting sustained biochemical remission of the disease after interferon therapy. Untreated patients with chronic hepatitis C showed increased … Show more

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Cited by 149 publications
(148 citation statements)
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“…7 To determine Mn-SOD mRNA levels we used a similar procedure. Either PBMC or liver total RNA (1.25 or 0.75 g, respectively) was reverse-transcribed (60 min at 37°C) with 200 U of M-MuLV reverse transcriptase (BRL, Gaithersburg, MD) in 25 l volume of 5ϫ RT buffer (0.25 mol/l Tris-HCl pH 8.3, 0.375 mol/l KCl, 15 mmol/l MgCl 2 ), supplemented with 5 mmol/l DTT, 0.4 mmol/l deoxynucleoside triphosphate, ribonuclease inhibitor (30 U), and random hexamers (250 ng) (Boehringer-Mannheim, Mannheim, Germany).…”
Section: Analysis Of Mrna Levels Of Mn-sod and Tnf〈 In Pbmc And In Thmentioning
confidence: 99%
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“…7 To determine Mn-SOD mRNA levels we used a similar procedure. Either PBMC or liver total RNA (1.25 or 0.75 g, respectively) was reverse-transcribed (60 min at 37°C) with 200 U of M-MuLV reverse transcriptase (BRL, Gaithersburg, MD) in 25 l volume of 5ϫ RT buffer (0.25 mol/l Tris-HCl pH 8.3, 0.375 mol/l KCl, 15 mmol/l MgCl 2 ), supplemented with 5 mmol/l DTT, 0.4 mmol/l deoxynucleoside triphosphate, ribonuclease inhibitor (30 U), and random hexamers (250 ng) (Boehringer-Mannheim, Mannheim, Germany).…”
Section: Analysis Of Mrna Levels Of Mn-sod and Tnf〈 In Pbmc And In Thmentioning
confidence: 99%
“…7 Mn-SOD cDNA fragments were amplified by 24 or 23 cycles (PBMC or liver, respectively) (94, 60, and 72°C, 1 min each step), and ␤-actin was amplified by 20 cycles (94, 55, and 72°C, 1 min each step), a protocol that avoided interference of the plateau effect. Oligonucleotides (5Ј-3Ј) d(TGCACCACAG-CAAGCACCAC) and d(AAACCAAGCCAAC-CCCAACC) were the upstream and downstream primers, respectively, used for amplification of 310 bp fragment from human Mn-SOD cDNA.…”
Section: Analysis Of Mrna Levels Of Mn-sod and Tnf〈 In Pbmc And In Thmentioning
confidence: 99%
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