Tumor marker-responsive behavior of gels prepared by biomolecular imprinting

Abstract

We report dynamic glycoprotein recognition of gels prepared by biomolecular imprinting using lectin and antibody molecules as ligands for tumor-specific marker glycoproteins. The glycoproteinimprinted gels prepared with minute amounts of cross-linkers could dynamically recognize tumor-specific marker glycoproteins by lectin and antibody ligands and induce volume changes according to the glycoprotein concentration. The glycoprotein-imprinted gel shrank in response to a target glycoprotein but nonimprinted gel swelled a little. The glycoprotein-responsive shrinking of the imprinted gel was caused by formation of lectin-glycoproteinantibody complexes that acted as reversible cross-linking points. Glycoprotein-imprinted gels only shrank when both lectin and antibody in the gels simultaneously recognized the saccharide and peptide chains of the target glycoprotein. As shrinking behavior of biomolecularly imprinted gels in response to glycoproteins enables the accurate detection and recognition of tumor-specific marker glycoproteins, they have many potential applications as smart devices in sensing systems and for molecular diagnostics.gel ͉ tumor-specific marker ͉ stimuli-responsive gel ͉ molecular recognition

A Thermosensitive Monolithic Column as an Artificial Antibody for the On‐line Selective Separation of the Protein

“…[29][30][31] So far, to the best of our knowledge, the preparation of a thermosensitive polymer to imprint a protein has been limited to static formats, such as our previous study on the bulk polymerization of a macroporous thermosensitive imprinted hydrogel for the recognition of proteins, [32] and has not been applied to monolithic columns for proteins. [33][34][35][36] This study focuses on the combined merits of the monolithic column and the thermosensitive protein MIP to selectively separate a template protein in an on-line method. NIsopropylacrylamide (NIPAAm; a smart material), acrylamide (AAm), and methacrylic acid (MAA) were chosen as monomers to develop a suitable thermosensitive MIP with lysozyme as the template.…”

Smart surface imprinting polymer nanospheres for selective recognition and separation of glycoprotein

“…Compared with other separation methods of glycoprotein [42], the imprinted nanospheres had a more satisfied selectivity. The adsorption rate of the imprinted nanospheres was faster than that of tumor marker-responsive gels [43], which can be attributed to the formation of the specific binding sites on the surface of the nanospheres. Moreover, the reusability was superior to that of MIPs by polymerization of 3-aminophenylboronic acid [44].…”

Smart polymeric gels: Redefining the limits of biomedical devices

“…Miyata et al in a recently reported article on stimuli-sensitive gels reported dynamic glycoprotein (GP) recognition by gels prepared by biomolecular imprinting [251]. The GPimprinted gels had lectin and antibodies as ligands for tumor-specific marker GPs and were prepared with minute amounts of crosslinkers.…”