Tumor growth activity of duloxetine in Ehrlich carcinoma in mice

Moura, Ed Carlos Rey; Leal, Plínio da Cunha; Serra, Izabel Cristina Portela Bogéa; Ribeiro, Bruno de Paulo; Nascimento, Johnny Ramos do; Nascimento, Flávia R.F.; Sakata, Rioko Kimiko

doi:10.1186/s13104-018-3655-4

Cited by 7 publications

(8 citation statements)

References 19 publications

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“…The tumor control group was treated with 12% (v/v) Tween-80 in saline. On the eighth day, the animals were anesthetized with ketamine (100 mg/kg) and xylazine hydrochloride (16 mg/kg), and then euthanized [25]. Total viable cancer cells were obtained as the product of the tumor volume (mL) by cell viability (number of cells × 10 6 /mL).…”

Section: In Vivo Antitumor Activitymentioning

confidence: 99%

Antitumor Effect of a Novel Spiro-Acridine Compound is Associated with Up-Regulation of Th1-Type Responses and Antiangiogenic Action

et al. 2019

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Tumor cells have specific features, including angiogenesis induction, cell cycle dysregulation, and immune destruction evasion. By inducing a T helper type 2 (Th2) immune response, tumor cells may favor immune tolerance within the tumor, which allows progression of cancer growth. Drugs with potential antitumor activity are the spiro-acridines, which is a promising new class of acridine compounds. Herein, the novel spiro-acridine (E)-5 -oxo-1 -((3,4,5-trimethoxybenzylidene)amino)-1 ,5 -dihydro-10H-spiro[acridine-9,2 -pyrrole]-4 -carbonitrile (AMTAC-17) was synthesized and tested for antitumor effects. Toxicity evaluation was performed in mice after acute treatment (2000 mg/kg, intraperitoneally, i.p.). The Ehrlich ascites carcinoma model was used to investigate the antitumor activity of AMTAC-17 (12.5, 25, or 50 mg/kg, i.p.) after seven days of treatment. Effects on the cell cycle, angiogenesis, and inflammatory responses were investigated. LD 50 (lethal dose 50%) was estimated to be higher than 5000 mg/kg. AMTAC-17 reduced the Ehrlich tumor's total viable cancer cells count and peritumoral micro-vessels density, and induced an increase in the sub-G1 peak. Additionally, there was an increase of Th1 cytokine profile levels (IL-1β, TNF-α, and IL-12). In conclusion, the spiro-acridine compound AMTAC-17 presents low toxicity, and its in vivo antitumor effect involves modulation of the immune system to a cytotoxic Th1 profile and a reduction of tumor angiogenesis.

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Section: In Vivo Antitumor Activitymentioning

confidence: 99%

Antitumor Effect of a Novel Spiro-Acridine Compound is Associated with Up-Regulation of Th1-Type Responses and Antiangiogenic Action

et al. 2019

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“…studies using animal models have found that behavioral stress stimulates the rapid progression of ovarian (46), pancreatic (47), prostate (48), and breast (49) carcinomas and malignant melanomas (50). Duloxetine is commonly used to treat depression and is suggested as an adjuvant therapy for cancer pain management (51).…”

Section: A549 Cells Were Also Pretreated With And/or Chloroquine (10 ...mentioning

confidence: 99%

Duloxetine Enhances TRAIL-mediated Apoptosis via AMPK-mediated Inhibition of Autophagy Flux in Lung Cancer Cells

Zinnah

Park

2019

Anticancer Res

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Background/Aim: The antidepressant duloxetine is known as a serotonin-norepinephrine reuptake inhibitor, used for treating depression and anxiety. TRAIL selectively induces cell death in a variety of tumor cells by binding to its membrane death receptor (DR). The aim of the study was to examine whether duloxetine affects TRAIL-mediated apoptosis. Materials and Methods: Cell viability and apoptosis was measured by morphological image, crystal violet staining, MTT and LDH assay. Immunocytochemistry and western blotting techniques were applied to detect autophagy and apoptosis indicator proteins. TEM assay was used to determine the autophagy. Results: Duloxetine treatment considerably sensitizes human lung adenocarcinoma cells to TRAIL-mediated apoptosis by targeting TRAIL-DR5. Treatment with duloxetine inhibited AMPK phosphorylation and resulted in increased p62 and microtubule-associated protein 1A/1B light chain 3B-II levels, indicating inhibition of autophagy flux. Blockade of DR5 with DR5-specific small-interfering RNA negatively regulated the apoptotic effect. Conclusion: Clinical administration of TRAIL in combination with duloxetine may serve as a therapeutic approach for the treatment of TRAILresistant lung cancer cells.

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“…Ascitic fluid was collected from donor mice after 7 days of inoculum, prepared with Trypan blue, and counted in a Neubauer chamber for viability assessment. Cells were diluted to 2×106 cells/mL and inoculated into mice by intraperitoneal injection [50] …”

Section: Methodsmentioning

confidence: 99%

Siparuna guianensis Essential Oil Antitumoral Activity on Ehrlich Model and Its Effect on Oxidative Stress

Viana Barbosa,

Silva de Jesus,

Botelho Jerônimo

et al. 2023

Chemistry & Biodiversity

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This work aims to evaluate the chemical composition, in vitro antioxidant capacity, and in vivo antitumoral activity of S. guianensis essential oil against Ehrlich's ascitic carcinoma and the effects on oxidative stress. The animals (Mus musculus) received a daily dose of S. guianensis oil orally (100 mg/kg) for 9 days. The main constituents of essential oil were curzerenone (16.4±1.5 %), drimenol (13.7±0.2 %), and spathulenol (12.4±0.8 %). S. guianensis oil showed antioxidant activity, inhibiting 11.1 % of DPPH radicals (95.7 mgTE/g); and 15.5 % of the β‐carotene peroxidation. The group treated with S. guianensis showed a significant reduction in tumor cells (59.76±12.33) compared to the tumor group (96.88±19.15). Essential oil of S. guianensis decreased MDA levels and increased SOD levels in liver tissue. The essential oil of S. guianensis reduced oxidative stress, and showed antitumor and antioxidant activity, being characterized as a new chemical profile in the investigation of pathologies such as cancer.

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Tumor growth activity of duloxetine in Ehrlich carcinoma in mice

Cited by 7 publications

References 19 publications

Antitumor Effect of a Novel Spiro-Acridine Compound is Associated with Up-Regulation of Th1-Type Responses and Antiangiogenic Action

Antitumor Effect of a Novel Spiro-Acridine Compound is Associated with Up-Regulation of Th1-Type Responses and Antiangiogenic Action

Duloxetine Enhances TRAIL-mediated Apoptosis via AMPK-mediated Inhibition of Autophagy Flux in Lung Cancer Cells

Siparuna guianensis Essential Oil Antitumoral Activity on Ehrlich Model and Its Effect on Oxidative Stress

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