Tumor detection using magnetosome nanoparticles functionalized with a newly screened EGFR/HER2 targeting peptide

Xiang, Zhichu; Yang, Xiaoliang; Xu, Junjie; Lai, Wenjia; Wang, Zihua; Hu, Zhiyuan; Tian, Jie; Geng, Li; Fang, Qiaojun

doi:10.1016/j.biomaterials.2016.11.022

Cited by 71 publications

(59 citation statements)

References 33 publications

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“…Bioengineered MTB are used to produce magnetosomes with diverse shapes and narrow distribution of crystal size as well as specific functions, which make them ideal as image contrasting agents. These custom‐tailored magnetosomes have been intensively investigated for MRI‐based detection of cancerous cells in experimental tumor models, [ 320–324 ] Bioengineering of MTB may be achieved using methods that include direct chemical modification, genetic engineering, and hybrid modification ( Figure A). With the advancement of nanotechnology, magnetosomes may be functionalized with different ligands such as enzymes, proteins, nucleic acids, green‐fluorescent protein, antibodies, and drugs (Figure 16B) [ 325 ] for multifunctional applications (Figure 16C).…”

Section: Applicationsmentioning

confidence: 99%

“…For example, chemical modification of magnetosomes with peptide P75 produces functionalized magnetosomes that bind to human epithelial growth factor receptor and epithelial growth factor receptor‐2. [ 327 ] Indirect interaction between magnetosome membrane and antibody may be achieved using a fusion tag. Staphylococcal protein A (SPA), which is an immunoglobulin G‐binding protein and serves as fusion tag, fuses with MamC or MamF and binds to the fragment crystallization (Fc region) of immunoglobulins.…”

Section: Applicationsmentioning

confidence: 99%

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Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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Bacteria play an important role in the calcification process of natural minerals and within organisms ( Table 1). It is Microbe-mediated mineralization is ubiquitous in nature, involving bacteria, fungi, viruses, and algae. These mineralization processes comprise calcification, silicification, and iron mineralization. The mechanisms for mineral formation include extracellular and intracellular biomineralization. The mineral precipitating capability of microbes is often harnessed for green synthesis of metal nanoparticles, which are relatively less toxic compared with those synthesized through physical or chemical methods. Microbe-mediated mineralization has important applications ranging from pollutant removal and nonreactive carriers, to other industrial and biomedical applications. Herein, the different types of microbe-mediated biomineralization that occur in nature, their mechanisms, as well as their applications are elucidated to create a backdrop for future research.

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Section: Applicationsmentioning

confidence: 99%

Section: Applicationsmentioning

confidence: 99%

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

et al. 2020

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“…Interestingly, magnetosomes can be functionalized in vivo by fusing foreign proteins of interest to the magnetosome membrane proteins, such as MamC/Mms13, MagA, and Mms16 (Arakaki et al, 2008;Ren et al, 2018). The translational fusion of functional proteins to these transmembrane proteins of magnetosomes has led to numerous successful prototypes in a wide range of applications, including of industrial uses (Ginet et al, 2011;Sugamata et al, 2013;Honda et al, 2015a,b;Xiang et al, 2017;Mickoleit and Schüler, 2018). The inherent magnetic characteristics of magnetosomes make them very useful in some situations, especially for implementation in magneticfield-related technologies, such as magneto-immunoassays and biomedical imaging (Ren et al, 2018).…”

Section: Magnetosomesmentioning

confidence: 99%

Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications

Wong

Ogura

Chen

et al. 2020

Front. Bioeng. Biotechnol.

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Enzymes function as biocatalysts and are extensively exploited in industrial applications. Immobilization of enzymes using support materials has been shown to improve enzyme properties, including stability and functionality in extreme conditions and recyclability in biocatalytic processing. This review focuses on the recent advances utilizing the design space of in vivo self-assembled polyhydroxyalkanoate (PHA) particles as biocatalyst immobilization scaffolds. Self-assembly of biologically active enzyme-coated PHA particles is a one-step in vivo production process, which avoids the costly and laborious in vitro chemical cross-linking of purified enzymes to separately produced support materials. The homogeneous orientation of enzymes densely coating PHA particles enhances the accessibility of catalytic sites, improving enzyme function. The PHA particle technology has been developed into a remarkable scaffolding platform for the design of cost-effective designer biocatalysts amenable toward robust industrial bioprocessing. In this review, the PHA particle technology will be compared to other biological supramolecular assembly-based technologies suitable for in vivo enzyme immobilization. Recent progress in the fabrication of biological particulate scaffolds using enzymes of industrial interest will be summarized. Additionally, we outline innovative approaches to overcome limitations of in vivo assembled PHA particles to enable finetuned immobilization of multiple enzymes to enhance performance in multi-step cascade reactions, such as those used in continuous flow bioprocessing.

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“…Recent research demonstrated the display of targeting peptides on the surfaces of these magnetosomes, such as RGD and recently the GFR/HER2 targeting peptide P75. [13][14][15][16] As targeting moieties, peptides offer several advantages, including their small size, high affinity, ease of modification, and low immunogenicity. [17][18][19][20] Despite their promise, some peptides are challenging to attach to the surface of synthetic iron oxide nanoparticles while maintaining their structural confirmation, making transgenic expression on the surface of magnetosomes the most direct means to produce contrast agents in such cases.…”

Section: Introductionmentioning

confidence: 99%

Genetic encoding of targeted MRI contrast agents for in vivo tumor imaging

Schuerle¹,

Furubayashi

Soleimany

et al. 2019

Preprint

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Tumor-selective contrast agents have the potential to aid in the diagnosis and treatment of cancer using noninvasive imaging modalities such as magnetic resonance imaging (MRI). Such contrast agents can consist of magnetic nanoparticles incorporating functionalities that respond to cues specific to tumor environments. Genetically engineering magnetotactic bacteria to display peptides has been investigated as a means to produce contrast agents that combine the robust image contrast effects of magnetosomes with transgenic targeting peptides displayed on their surface. This work reports the first use of magnetic nanoparticles that display genetically-encoded pH low insertion peptide (pHLIP), a long peptide intended to enhance MRI contrast by targeting the extracellular acidity associated with the tumors. To demonstrate the modularity of this versatile platform to incorporate diverse targeting ligands by genetic engineering, we also incorporated the cyclic αv integrin-binding peptide iRGD into separate magnetosomes. Specifically, we investigate their potential for enhanced binding and tumor imaging both in vitro and in vivo. Our experiments indicate that these tailored magnetosomes retain their magnetic properties, making them well-suited as T2 contrast agents, while exhibiting increased binding compared to wild-type magnetosomes.

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Tumor detection using magnetosome nanoparticles functionalized with a newly screened EGFR/HER2 targeting peptide

Cited by 71 publications

References 33 publications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Microbe‐Mediated Extracellular and Intracellular Mineralization: Environmental, Industrial, and Biotechnological Applications

Bioengineered Polyhydroxyalkanoates as Immobilized Enzyme Scaffolds for Industrial Applications

Genetic encoding of targeted MRI contrast agents for in vivo tumor imaging

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