Tumor Cell Behavior in Porous Hydrogels: Effect of Application Technique and Doxorubicin Treatment

Zoughaib, Mohamed; Luong, Duong; Siraeva, Zulfira; Yergeshov, Abdulla A.; Salikhova, Taliya I.; Кузнецова, С. В.; Kiyamova, Ramziya; Abdullin, Timur I.

doi:10.1007/s10517-019-04577-y

Cited by 8 publications

(9 citation statements)

References 28 publications

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“…According to LSCM analysis, the TM-doped cryogels preserved an interconnected porous structure ( Figure 1 A), typical of cryogel scaffolds, with somewhat larger pores at the upper surface, commonly used for cell culture [ 34 ]. Non-doped cryogels possessed macropores with a calculated mean pore size of 80 ± 13 μm, which moderately decreased by 1.2–1.4 times in the TM-doped materials.…”

Section: Resultsmentioning

confidence: 99%

“…The cryogels with cultured cells were collected at day 3, transferred into new wells containing 0.5 mL MTS/PMS reagents in fresh culture medium to assess cell metabolic activity [ 34 ]. After incubation for 1.5 h under standard culture conditions (37 °C, 5% CO 2 ), the absorbance was determined at 490 nm on an Infinite M200 PRO microplate analyzer (Tecan).…”

Section: Methodsmentioning

confidence: 99%

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Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Yergeshov

Zoughaib

Ishkaeva

et al. 2022

Gels

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Divalent trace metals (TM), especially copper (Cu), cobalt (Co) and zinc (Zn), are recognized as essential microelements for tissue homeostasis and regeneration. To achieve a balance between therapeutic activity and safety of administered TMs, effective gel formulations of TMs with elucidated regenerative mechanisms are required. We studied in vitro and in vivo effects of biodegradable macroporous cryogels doped with Cu, Co or Zn in a controllable manner. The extracellular ROS generation by metal dopants was assessed and compared with the intracellular effect of soluble TMs. The stimulating ability of TMs in the cryogels for cell proliferation, differentiation and cytokine/growth factor biosynthesis was characterized using HSF and HUVEC primary human cells. Multiple responses of host tissues to the TM-doped cryogels upon subcutaneous implantation were characterized taking into account the rate of biodegradation, production of HIF-1α/matrix metalloproteinases and the appearance of immune cells. Cu and Zn dopants did not disturb the intact skin organization while inducing specific stimulating effects on different skin structures, including vasculature, whereas Co dopant caused a significant reorganization of skin layers, the appearance of multinucleated giant cells, along with intense angiogenesis in the dermis. The results specify and compare the prooxidant and regenerative potential of Cu, Co and Zn-doped biodegradable cryogels and are of particular interest for the development of advanced bioinductive hydrogel materials for controlling angiogenesis and soft tissue growth.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Yergeshov

Zoughaib

Ishkaeva

et al. 2022

Gels

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“…[ [25][26][27][28][29] HEMA-Gelatin: radical polymerization and cross-linking with poly(ethylene glycol) diacrylate (PEGDA) [30] Reactions of multifunctional-amine and epoxy groups; chitosan and diglycidyl ethers of ethylene glycol (EGDGE) or polyethylene glycol (PEGDGE) [31] Reaction between amine and carboxy groups: gelatin, gelatin-heparin cross-linked with EDC/NHS [32,33] Oxime-cross-linking of gelatin-oxyamine and hyaluronan-aldehyde; gelatin-hyaluronan cryogel [34] Radiation crosslinking, exposure to high energy source (electron beam)…”

Section: Ionic Interactionmentioning

confidence: 99%

“…Facilitates cancer cell invasion, migration and proliferation allowing for comprehensive characteristic of tumor cell behavior and testing the activity of anticancer drugs [29] Hyaluronic acid-PEG Immobilized biomimetic ligands offer control over cellular response resulting in enhanced spreading with RGD and formation of 3D cell spheroids with IKVAV [161] HEMA-Alginate-Gelatin 3D culturing of prostate cancer cells and spheroids to study their progression and behavior [188] Poly(ethylene glycol) diacrylate and type I collagen semi-interpenetrating polymer network cryogels Tunable off-the-shelf platforms for 3D cancer cell [112] Drug delivery HEMA-Gelatin pH-responsive release of doxorubicin with increased rate in more acidic conditions mimicking tumor environment [30] Inulin cryogel Release of amoxicillin trihydrate [85] Alginate beads Release of quercetin as antioxidant [19] Chitosan, hydroxyapatite, heparin, and polyvinyl alcohol composite Release of bone morphogenic protein 2 and support of the differentiation of rat bone marrow mesenchymal stem cells.…”

Section: Gelatinmentioning

confidence: 99%

Design and Assessment of Biodegradable Macroporous Cryogels as Advanced Tissue Engineering and Drug Carrying Materials

Zoughaib

Yergeshov

2021

Gels

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Cryogels obtained by the cryotropic gelation process are macroporous hydrogels with a well-developed system of interconnected pores and shape memory. There have been significant recent advancements in our understanding of the cryotropic gelation process, and in the relationship between components, their structure and the application of the cryogels obtained. As cryogels are one of the most promising hydrogel-based biomaterials, and this field has been advancing rapidly, this review focuses on the design of biodegradable cryogels as advanced biomaterials for drug delivery and tissue engineering. The selection of a biodegradable polymer is key to the development of modern biomaterials that mimic the biological environment and the properties of artificial tissue, and are at the same time capable of being safely degraded/metabolized without any side effects. The range of biodegradable polymers utilized for cryogel formation is overviewed, including biopolymers, synthetic polymers, polymer blends, and composites. The paper discusses a cryotropic gelation method as a tool for synthesis of hydrogel materials with large, interconnected pores and mechanical, physical, chemical and biological properties, adapted for targeted biomedical applications. The effect of the composition, cross-linker, freezing conditions, and the nature of the polymer on the morphology, mechanical properties and biodegradation of cryogels is discussed. The biodegradation of cryogels and its dependence on their production and composition is overviewed. Selected representative biomedical applications demonstrate how cryogel-based materials have been used in drug delivery, tissue engineering, regenerative medicine, cancer research, and sensing.

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“…In vitro drug delivery properties of dual-targeted RGD/GHK liposomes were assessed using doxorubicin (DOX) and paclitaxel (PTX) as well-established anticancer/antiangiogenic drugs. 42,43 DOX was encapsulated into the liposomes from the aqueous phase, whereas PTX was pre-distributed in the lipid phase. EE of the drugs was determined by spectrophotometric and HPLC analyses, respectively.…”

Section: Study Of Targeted Liposomal Formulations Of Anticancer Drugsmentioning

confidence: 99%

Amphiphilic RGD and GHK peptides synergistically enhance liposomal delivery into cancer and endothelial cells

et al. 2021

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Tumor Cell Behavior in Porous Hydrogels: Effect of Application Technique and Doxorubicin Treatment

Cited by 8 publications

References 28 publications

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Regenerative Activities of ROS-Modulating Trace Metals in Subcutaneously Implanted Biodegradable Cryogel

Design and Assessment of Biodegradable Macroporous Cryogels as Advanced Tissue Engineering and Drug Carrying Materials

Amphiphilic RGD and GHK peptides synergistically enhance liposomal delivery into cancer and endothelial cells

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