Tumor-bone interaction induced by transplantable human tumors in nude mice

Abstract

Tumor-bone interactions were experimentally studied using four transplantable human urogenital tumors in nude mice. The method consisted of subcutaneously (SC) inoculating tumor cells over the calvaria in nude mice after the periosteum has been disrupted. This resulted in a local tumor causing fragmentation of the bone. The degree of tumor-bone interaction also varied with the type of implanted tumors as shown in radiographic and histologic examinations. All tumors were associated with histologic patterns of classical bone remodeling, including bone destruction with osteoclast proliferation and reactive new bone formation. The evidence presented here suggests that the majority of tumor-bone interaction showed a combination of both features, bone destruction and new bone formation, and the mechanisms whereby tumors interact with bone may vary with the biological properties of the tumor. Our new system would be suitable for studying the biology of local interaction between bone and tumor cells and searching out a method to protect the bone from cancer cells.

Inhibition by a new bisphosphonate (YM175) of bone resorption induced by the MBT-2 tumour of mice

“…With regard to experimental model systems of neoplasmassociated local osteolysis, tumour-bone interactions using calvaria of the mice were extensively studied by our group (Nemoto et al, 1986;Nemoto et al, 1987;Nemoto et al, 1988a;Nemoto et al, 1988b). In one of the recent studies, suggestive evidence was obtained that our system might be suitable for studying the biology of local interaction between bone and cancer cells.…”

“…The method consisted of inoculating tumour cells subcutaneously (SC) (Fleisch et al, 1969;Fleisch & Felix, 1979;Fleisch, 1983). Inhibition of tumour induced osteolysis by bisphosphonates has been noted in animal and human studies (Jung et al, 1981; VanHoltenVerzantvoort et al, 1987;Morton & Howell, 1988).With regard to experimental model systems of neoplasmassociated local osteolysis, tumour-bone interactions using calvaria of the mice were extensively studied by our group (Nemoto et al, 1986; Nemoto et al, 1987;Nemoto et al, 1988a;Nemoto et al, 1988b). In one of the recent studies, suggestive evidence was obtained that our system might be suitable for studying the biology of local interaction between bone and cancer cells.…”

“…The tumour has retained the histologic appearance of a poorly differentiated transitional cell carcinoma. TSU-PRI is a cell line derived from a primary adenocarcinoma of the prostate in a 73-year-old man with multiple osteoblastic bone metastases (Nemoto et al, 1988b).Induction of osteolysis MBT-2 cells were transplanted routinely by subcutaneously (SC) inoculation into female C3H/He mice. When the resulting tumour was visible, it was excised aseptically and minced in medium 199.…”

“…The tumour has retained the histologic appearance of a poorly differentiated transitional cell carcinoma. TSU-PRI is a cell line derived from a primary adenocarcinoma of the prostate in a 73-year-old man with multiple osteoblastic bone metastases (Nemoto et al, 1988b).…”

“…The tumour has retained the histologic appearance of a poorly differentiated transitional cell carcinoma. TSU-PRI is a cell line derived from a primary adenocarcinoma of the prostate in a 73-year-old man with multiple osteoblastic bone metastases (Nemoto et al, 1988b Radiographs were examined in blind conditions. The area of bone resorption from radiograph detectable lesions was measured by computerised analysis using graphtec software for plotters.…”