Tumor-associated carbohydrate antigens in primary pulmonary adenocarcinomas and their metastases

Sugiyama, Keisaku; Kawai, Toshiaki; Nagata, Noriko; Suzuki, Minoru

doi:10.1016/0046-8177(92)90402-o

Cited by 14 publications

(5 citation statements)

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“…Thus, when cancer cells express CA19-9, they appear to play an important role in the process of haematogenous metastasis (Takada et al, 1991(Takada et al, , 1993. Several immunohistochemical studies have demonstrated that expression of CA19-9 is correlated with a high risk of distant metastasis and poor outcome in lung cancer patients (Sugiyama et al, 1992, Ogawa et al, 1994. This evidence suggests strongly that CA19-9 also plays a role in metastatic processes, in vivo.…”

Section: Discussionmentioning

confidence: 99%

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

et al. 1999

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Summary It has been reported that cytokeratin 8 (CK8) is expressed in all non-small-cell lung cancers (NSCLC). We hypothesized that antigenic changes of CK8 may occur in some NSCLC cell lines. To prove this, Western immunoblot analysis using anti-human CK8 monoclonal antibodies as well as immunohistological staining of CK8 were performed in NSCLC cell lines. As a result, CK8 which had a higher molecular weight than recombinant CK8 was demonstrated in two of eight NSCLC cell lines. In addition, this CK8 contained antigenic epitopes of CA19-9. This CK8 with higher molecular weight, may have played a role in the process of invasion or metastasis of NSCLC. © 1999 Cancer Research CampaignKeywords: antibody; CA19-9; cytokeratin 8; non-small-cell lung cancer 769British Journal of Cancer (1999) 81(5), 769-773 © 1999 Cancer Research Campaign Article no. bjoc.1999 Received 24 November 1998 Revised 28 April 1999 Accepted 10 May 1999Correspondence to: J Fujita Proteins were electrophoretically transferred onto nitrocellulose membrane by the method of Towbin et al (1983). Proteins were detected by immunoblotting, using five clones of anti-CK8 monoclonal antibodies (clones Ks 8.7, Ks 8.10, Ks 8.17.2, and M20 were purchased from Progen Biotechnik GMBH, Heidelberg, Germany; clone C-51 was purchased from YLEM S. r. 1., Roma, Italy), peroxidase-conjugated goat anti-mouse IgG antibody (Sigma ImmunoChemicals, lot 094H-4810, St Louis, MO, USA), and stained with 4CN PLUS for chromogenic detection of horseradish peroxidase (NEM TM Life Science Products, Boston, MA, USA). Western immunoblots for CA19-9 were also performed using three clones of anti-CA19-9 monoclonal antibodies (clone C241:5:1:4 was purchased from YLEM S. r. 1., Roma, Italy; clone ZY-CO9 was purchased from Zymed Laboratories, Inc., San Francisco, CA, USA; clone 1116-NS-19-9 was purchased from Centocor Co., Malvern, PA, USA), and peroxidase-conjugated goat anti-mouse IgG antibody IgG antibody, and stained with 4CN PLUS. Immunohistochemistry of lung cancer cells by several anti-CK8 antibodiesTo evaluate the expression of CK8 in several NSCLC cell lines, immunohistochemical staining by anti-human monoclonal antibody against CK8 was performed. Cells were immunohistochemically stained, employing the avidin-biotin peroxidase complex method (Dako LSAB kit-peroxidase, DAKO Corp., Kyoto, Japan) using mouse monoclonal antibodies against CK8 (clone 35 βH11, Enzo Diagnostics, Inc., New York, NY, USA, 1:5000 dilution). In order to retrieve and increase the immunoreactivities, preincubation with 0.1% pronase at 37°C for 20 min was performed. Lectin blottingSDS-PAGE and protein transfer onto nitrocellulose membrane was performed as described above. Ten kinds of horseradish peroxidase-labelled lectins, purchased from EY Laboratories, Inc. (San Mateo, CA, USA), were used for lectin blotting. Proteins were detected by ten kinds of peroxidase-labelled lectins (1:100 dilutions, following manufacturer's instruction), and stained with 4CN PLUS. Lectins used were as follows; Maclura pomifer...

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Section: Discussionmentioning

confidence: 99%

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

et al. 1999

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“…LeX-terminated glycosphingolipids and glycoproteins are highly expressed in a variety of human cancers [17][18][19]. Fvirosomes by virtue of LeX-Le x interaction may find their use as a potent carrier of cytosol active anticancer drugs.…”

Section: Discussionmentioning

confidence: 99%

F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through Le^X‐Le^X interaction

Kumar

Sarkar

1996

FEBS Letters

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“…The expression of sLe (x) has been reported in several human cancer cells, such as colorectal carcinoma [18][19][20][21][22]33], gastric carcinoma [12,25,30], pulmonary adenocarcinoma [27,29] and squamous cell carcinoma [6,13]. The expression of sLe (x) in both primary and metastatic lesions of human breast carcinomas was also reported and the relationship between the metastatic behavior of the tumor and expression of sLe (x) was implied [24].…”

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The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

et al. 2002

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The expression of sialyl Lewis X (sLe(x)) in 93 canine and 15 feline mammary gland tumors (MGT) obtained by surgical resection at Veterinary Medical Center, the University of Tokyo was examined by immunohistochemistry. Their clinicopathological features and prognosis were also reviewed. Approximately 60% of MGT tissues showed sLe(x) positive expressions, while all normal mammary gland tissues were negative. However, its expression was not correlated with clinicopathological features and prognosis significantly. This study suggests that sLe(x) may be a tumor-associated antigen in canine and feline MGTs.

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Tumor-associated carbohydrate antigens in primary pulmonary adenocarcinomas and their metastases

Cited by 14 publications

References 5 publications

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through Le^X‐Le^X interaction

The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

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Tumor-associated carbohydrate antigens in primary pulmonary adenocarcinomas and their metastases

Cited by 14 publications

References 5 publications

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

Detection of large molecular weight cytokeratin 8 as carrier protein of CA19–9 in non-small-cell lung cancer cell lines

F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through LeX‐LeX interaction

The Expression of Sialyl Lewis X in Canine and Feline Mammary Gland Tumors.

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F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through Le^X‐Le^X interaction