Tumeurs du sac vitellin de l’ovaire : revue de la littérature

Even, Caroline; Lhommé, C.; Duvillard, P.; Morice, Philippe; Balleyguier, Corinne; Pautier, Patricia; Troalen, Frédéric; Rouge, Thibault De La Motte

doi:10.1684/bdc.2011.1387

Cited by 6 publications

(4 citation statements)

References 77 publications

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“…Yolk sac carcinoma are the most common malignant germ cell tumors in children, which are commonly found in the ovary, testes, sacrococcygeal areas and the midline of the body [1-4]. This type of germ tumors is aggressive and highly metastatic which can rapidly spread to adjoining tissues through the lymphatic system [5-7].…”

Section: Introductionmentioning

confidence: 99%

Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells

Hua

Liu

et al. 2011

J Exp Clin Cancer Res

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BackgroundMDR1 gene encoding P-glycoprotein is an ATP-dependent drug efflux transporter and related to drug resistance of yolk sac carcinoma. Ultrasound microbubble-mediated delivery has been used as a novel and effective gene delivery method. We hypothesize that small interfering RNA (siRNA) targeting MDR1 gene (siMDR1) delivery with microbubble and ultrasound can down-regulate MDR1 expression and improve responsiveness to chemotherapeutic drugs for yolk sac carcinoma in vitro.MethodsRetroviral knockdown vector pSEB-siMDR1s containing specific siRNA sites targeting rat MDR1 coding region were constructed and sequence verified. The resultant pSEB-siMDR1 plasmids DNA were encapsulated with lipid microbubble and the DNA release were triggered by ultrasound when added to culture cells. GFP positive cells were counted by flow cytometry to determine transfection efficiency. Quantitative real-time PCR and western blot were performed to determine the mRNA and protein expression of MDR1. P-glycoprotein function and drug sensitivity were analyzed by Daunorubicin accumulation and MTT assays.ResultsTransfection efficiency of pSEB-siMDR1 DNA was significantly increased by ultrasound microbubble-mediated delivery in rat yolk sac carcinoma L2 (L2-RYC) cells. Ultrasound microbubble-mediated siMDR1s delivery effectively inhibited MDR1 expression at both mRNA and protein levels and decreased P-glycoprotein function. Silencing MDR1 led to decreased cell viability and IC50 of Vincristine and Dactinomycin.ConclusionsOur results demonstrated that ultrasound microbubble-mediated delivery of MDR1 siRNA was safe and effective in L2-RYC cells. MDR1 silencing led to decreased P-glycoprotein activity and drug resistance of L2-RYC cells, which may be explored as a novel approach of combined gene and chemotherapy for yolk sac carcinoma.

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Section: Introductionmentioning

confidence: 99%

Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells

Hua

Liu

et al. 2011

J Exp Clin Cancer Res

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“…Yolk sac tumors of the ovary are rare tumors that usually occur in young women 20 -30 years old [1] [2] [3]. They represent the second most common histologic subtype of malignant ovarian germ cell tumor after dysgerminomas [4].…”

Section: Discussionmentioning

confidence: 99%

“…A few years later, Teilum reclassified these tumors and described their extraembryonal germ cell origin. He designated them as endodermal sinus tumors, which were termed later "Yolk sac tumor" due to their similarities with the extraembryonal yolk sac and vitelline structures [2] [3].…”

Section: Introductionmentioning

confidence: 99%

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Refractory Ascites Revealing an Ovarian Yolk Sac Tumor with Intraperitoneal Rupture

Razafimahefa¹,

Razafindrafara²,

Rafaralahivoavy³

et al. 2023

OJPathology

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Yolk sac tumors of the ovary are rare entities that account for 2% -5% of all ovarian tumors. They represent the second most common histological variant of malignant germ cell tumors of the ovary after dysgerminomas. Yolk sac tumors are most commonly encountered in women in the second and third decades. Microscopically, they are highly polymorphic and can present in a pure form or associated with another contingent of germ cell tumor. We report the case of a 26-year-old woman, who underwent surgery for a large right ovarian tumor rupturing into the peritoneal cavity. The ovarian tumor was revealed by ascites of great abundance and abdomino-pelvic pain. On histological examination, the diagnosis of yolk sac tumor in its pure and polyvesicular vitelline pattern was made. Through this observation, we propose to discuss the anatomoclinical particularities of these tumors by emphasizing the importance of histology for the diagnosis as well as the need of an early and appropriate management.

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Maternal Tumours Associated with and Influenced by Pregnancy

Berencsi

2012

Maternal Fetal Transmission of Human Viruses and Their Influence on Tumorigenesis

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Tumeurs du sac vitellin de l’ovaire : revue de la littérature

Cited by 6 publications

References 77 publications

Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells

Ultrasound microbubble-mediated delivery of the siRNAs targeting MDR1 reduces drug resistance of yolk sac carcinoma L2 cells

Refractory Ascites Revealing an Ovarian Yolk Sac Tumor with Intraperitoneal Rupture

Maternal Tumours Associated with and Influenced by Pregnancy

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