Tumeur à rénine. À propos d'un nouveau cas diagnostiqué au cours d'une grossesse

Ducret, F; Turc-Baron, C.; Pointet, P; Vernin, Guillaume; Skowron, Olivier; Gregor, Brigitte Mc; Gasc, Jean‐Marie; Beaune, G.; Vincent, Madeleine

doi:10.1016/j.nephro.2005.01.008

Cited by 3 publications

(3 citation statements)

References 30 publications

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“…Mut3DPT‐ C 9 h is an improved variant of the original DPT‐ C 9 h CPP that has increased stability in mouse serum while keeping the same pro‐apoptotic properties . However, cancer cells treated with the therapeutic peptide C 9 h without being coupled to a delivery system at concentrations as high as 100 μ m showed the same apoptosis levels as non‐treated cells, because this peptide is not capable of being internalized in the cells without a vehicle .…”

Section: Resultsmentioning

confidence: 99%

“…Apoptosis is a genetically programmed form of cell death whose dysregulation is associated with cancers. Recently, several phosphatases have become attractive targets for the treatment of a variety of diseases, including cancers . However, the only clinical drugs targeting phosphatases are the immunosuppressive cyclosporine A and FK506, which inhibit serine/threonine phosphatase 2B (calcineurin) and NFAT activation .…”

Section: Introductionmentioning

confidence: 99%

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ϵ‐Polylysine‐Capped Mesoporous Silica Nanoparticles as Carrier of the C9h Peptide to Induce Apoptosis in Cancer Cells

Torre

Dominguez-Berrocal

Murguía

et al. 2018

Chemistry A European J

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Apoptotic signaling pathways are altered in numerous pathologies such as cancer. In this scenario, caspase-9/PP2Acα interaction constitutes a key target with pharmacological interest to re-establish apoptosis in tumor cells. Very recently, a short peptide (C9h) known to disrupt caspase-9/PP2Acα interaction with subsequent apoptosis induction was described. Here, we prepared two sets of mesoporous silica nanoparticles loaded with safranin O (S2) or with C9h peptide (S4) and functionalized with ϵ-polylysine as capping unit. Aqueous suspensions of both nanoparticles showed negligible cargo release whereas in the presence of pronase, a marked delivery of safranin O or C9h was observed. Confocal microscopy studies carried out with HeLa cells indicated that both materials were internalized and were able to release their entrapped cargos. Besides, a marked decrease in HeLa cell viability (ca. 50 %) was observed when treated with C9h-loaded S4 nanoparticles. Moreover, S4 provides peptide protection from degradation additionally allowing for a dose reduction to observe an apoptotic effect when compared with C9h alone or in combination with a cell-penetrating peptide (i.e., Mut3DPT-C9h). Flow cytometry studies, by means of Annexin V-FITC staining, showed the activation of apoptotic pathways in HeLa as a consequence of S4 internalization, release of C9h peptide and disruption of caspase-9/PP2Acα interaction.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ϵ‐Polylysine‐Capped Mesoporous Silica Nanoparticles as Carrier of the C9h Peptide to Induce Apoptosis in Cancer Cells

Torre

Dominguez-Berrocal

Murguía

et al. 2018

Chemistry A European J

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show abstract

“…Several phosphatases have recently become attractive targets for the treatment of a variety of diseases, including cancers [1], [2], [3], [4]. However, the only clinical drugs targeting a phosphatase are the immunosuppresssive cyclosporin A and FK506, which inhibit Serine/Threonine phosphatase 2B (calcineurin) and NFAT activation [5], [6], [7], [8], [9].…”

Section: Introductionmentioning

confidence: 99%

Specific Targeting of Caspase-9/PP2A Interaction as Potential New Anti-Cancer Therapy

et al. 2013

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PurposePP2A is a serine/threonine phosphatase critical to physiological processes, including apoptosis. Cell penetrating peptides are molecules that can translocate into cells without causing membrane damage. Our goal was to develop cell-penetrating fusion peptides specifically designed to disrupt the caspase-9/PP2A interaction and evaluate their therapeutic potential in vitro and in vivo.Experimental DesignWe generated a peptide containing a penetrating sequence associated to the interaction motif between human caspase-9 and PP2A (DPT-C9h), in order to target their association. Using tumour cell lines, primary human cells and primary human breast cancer (BC) xenografts, we investigated the capacity of DPT-C9h to provoke apoptosis in vitro and inhibition of tumour growth (TGI) in vivo. DPT-C9h was intraperitonealy administered at doses from 1 to 25 mg/kg/day for 5 weeks. Relative Tumour Volume (RTV) was calculated.ResultsWe demonstrated that DPT-C9h specifically target caspase-9/PP2A interaction in vitro and in vivo and induced caspase-9-dependent apoptosis in cancer cell lines. DPT-C9h also induced significant TGI in BC xenografts models. The mouse-specific peptide DPT-C9 also induced TGI in lung (K-Ras model) and breast cancer (PyMT) models. DPT-C9h has a specific effect on transformed B cells isolated from chronic lymphocytic leukemia patients without any effect on primary healthy cells. Finally, neither toxicity nor immunogenic responses were observed.ConclusionUsing the cell-penetrating peptides blocking caspase-9/PP2A interactions, we have demonstrated that DPT-C9h had a strong therapeutic effect in vitro and in vivo in mouse models of tumour progression.

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Tumeur à rénine, une cause rare d’hypertension artérielle curable : à propos d’un cas

Ruyer

Millet

Ribstein

et al. 2011

Journal de Radiologie

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Tumeur à rénine. À propos d'un nouveau cas diagnostiqué au cours d'une grossesse

Cited by 3 publications

References 30 publications

ϵ‐Polylysine‐Capped Mesoporous Silica Nanoparticles as Carrier of the C9h Peptide to Induce Apoptosis in Cancer Cells

ϵ‐Polylysine‐Capped Mesoporous Silica Nanoparticles as Carrier of the C9h Peptide to Induce Apoptosis in Cancer Cells

Specific Targeting of Caspase-9/PP2A Interaction as Potential New Anti-Cancer Therapy

Tumeur à rénine, une cause rare d’hypertension artérielle curable : à propos d’un cas

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