Tularemia in Denmark: Identification of a
            <i>Francisella tularensis</i>
            subsp.
            <i>holarctica</i>
            Strain by Real-Time PCR and High-Resolution Typing by Multiple-Locus Variable-Number Tandem Repeat Analysis

Byström, Mona; Böcher, S.; Magnusson, Anna; Prag, Jørgen; Johansson, Anders

doi:10.1128/jcm.43.10.5355-5358.2005

Cited by 42 publications

(44 citation statements)

References 18 publications

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“…In Belgium there have been reports of tularemia in wildlife, but not in humans, either in the databases or the literature. In Denmark, the only wildlife case reported was in a European brown hare, and there was a single human case described in the literature [72]. Tularemia cases in domestic animals have been reported from Bulgaria, the Czech Republic, Germany, Finland, France, Italy, Norway, Poland, Slovakia, Spain, Sweden and Switzerland.…”

Section: Geographical Distribution Of Tularemia In Europe 1992-2012mentioning

confidence: 99%

The status of tularemia in Europe in a one-health context: a review

et al. 2014

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SUMMARYThe bacterium Francisella tularensis causes the vector-borne zoonotic disease tularemia, and may infect a wide range of hosts including invertebrates, mammals and birds. Transmission to humans occurs through contact with infected animals or contaminated environments, or through arthropod vectors. Tularemia has a broad geographical distribution, and there is evidence which suggests local emergence or re-emergence of this disease in Europe. This review was developed to provide an update on the geographical distribution of F. tularensis in humans, wildlife, domestic animals and vector species, to identify potential public health hazards, and to characterize the epidemiology of tularemia in Europe. Information was collated on cases in humans, domestic animals and wildlife, and on reports of detection of the bacterium in arthropod vectors, from 38 European countries for the period 1992-2012. Multiple international databases on human and animal health were consulted, as well as published reports in the literature. Tularemia is a disease of complex epidemiology that is challenging to understand and therefore to control. Many aspects of this disease remain poorly understood. Better understanding is needed of the epidemiological role of animal hosts, potential vectors, mechanisms of maintenance in the different ecosystems, and routes of transmission of the disease.

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Section: Geographical Distribution Of Tularemia In Europe 1992-2012mentioning

confidence: 99%

The status of tularemia in Europe in a one-health context: a review

et al. 2014

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“…4,7,10,19 Sequence analysis of the single nucleotide polymorphism markers B.18 (derived state) and B.19 (ancestral state) confirmed the subclade B4.FTNF002-00, 21 which is endemic in Switzerland. Multiple-loci variable number of tandem repeats (VNTR) analysis 2,13,19 was performed, and the isolated strain showed a VNTR profile that is circulating in Switzerland. 19 Awareness of tularemia is emerging in Europe, and increased resources are being invested in order to better understand this disease.…”

Section: Research-article2013mentioning

confidence: 99%

Francisella tularensisinfection in a stone marten (Martes foina) without classic pathological lesions consistent with tularemia

Origgi

Pilo

2013

J VET Diagn Invest

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The current report describes the isolation and typing of a strain of Francisella tularensis, the causative agent of tularemia, from the spleen of a stone marten ( Martes foina) showing no classic lesions consistent with the disease. The identification of this bacterium, belonging to the World Health Organization risk 3 category and considered to have a low infectious dose, could be performed only because of an ongoing project screening F. tularensis in the environment sensu lato. The findings described herein should alert diagnostic laboratories of the possible presence of F. tularensis in clinical samples in countries where tularemia is endemic even in cases with no consistent anamnesis and from unsuspected animal species.

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“…The genomes of subspecies holarctica strains universally exhibit a specific 30-bp deletion at the targeted genomic locus (Johansson et al, 2004;Byström et al, 2005). The PCR assay relies on amplification of Ft-M19 that is located between the genes deaD and ppiC using a pair of amplification primers that are located inside the flanking genes.…”

Section: B2 Molecular Biotypingmentioning

confidence: 99%

“…These PCR assays can provide F. tularensis subspecies identification based on size differences in ampli fied DNA products. Molecular subtyping assays are directed against an upstream region of an RNA helicase (the Ft-M19 assay), the presence or absence of the ISFtu2 insertion sequence element (ISFtu2 assay), the F. tularensis region of difference 1 (RD1 assay) and the pdpD gene (pdpD-2 assay) (Johansson et al, 2000c;Broekhuijsen et al, 2003;Johansson et al, 2004;Nano et al, 2004;Petersen et al, 2004a;Byström et al, 2005). Primer sequences and sizes of amplified gene products for the subspecies are given in Table 6.2 and protocol B.2.…”

Section: Molecular Biotypingmentioning

confidence: 99%

“…B.2.1 Gel-based PCR for identification of F. tularensis subspecies holarctica (type B) strains (Byström et al, 2005) The targeted F. tularensis DNA marker has been denoted (Ft-M19) and is present in all four F. tularensis subspecies: tularensis (type A), holarctica (type B), mediasiatica, and novicida. The genomes of subspecies holarctica strains universally exhibit a specific 30-bp deletion at the targeted genomic locus (Johansson et al, 2004;Byström et al, 2005).…”

Section: B2 Molecular Biotypingmentioning

confidence: 99%

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