Tularemi Lenfadeniti Şüphesi ile Alınan Lenf Aspiratı Örneklerinde Mycobacterium tuberculosis Varlığının Araştırılması

Albayrak, Nurhan; Çelebі, Bekіr; Kavas, Semra Toprak; Şimşek, Hülya; Kılıç, Selçuk; Sezen, Figen; Arslantürk, Ahmet

doi:10.5578/mb.6007

Cited by 3 publications

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“…Following M. simiae, M. fortuitum , and M. abscessus were the next most frequently isolated species in our study. Similar findings have been reported in other studies, such as those conducted by Sharma et al [29], and Shenai et al[30] in India, Albayrak et al in Turkey [31], and Ahmed et al in Pakistan [32]. In Taiwan, Greece, and the United Kingdom, M. fortuitum is the second most commonly isolated NTM species after members of the M. avium complex (MAC), with percentages of 23%, 21%, and 20%, respectively [33].…”

Section: Discussionsupporting

confidence: 91%

Species and Genetic Diversity of Nontuberculous Mycobacteria (NTM) in Suspected Tuberculosis Cases in East Azerbaijan, Iran: A Cross-sectional Analysis

Roshdi Maleki

2024

Preprint

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IntroductionThe outbreak of nontuberculous mycobacterial (NTM) infections has increased worldwide, attracting attention in routine diagnostic settings, particularly among patients with suspected tuberculosis. This study aimed to acquire knowledge of NTM infections in patients with suspected tuberculosis and to evaluate the genetic diversity of the strains.MethodsIn this study, 230 clinical specimens were collected from suspected tuberculosis patients. Following decontamination with N-Acetyl-L-cysteine–sodium hydroxide (NALC-NaOH), the sediments of specimens were inoculated onto Löwenstein–Jensen medium and then incubated at 30°C for 8 weeks. The samples that yielded positive cultures underwent evaluation through sequencing conserved fragments ofIS6110andhsp65. For those samples that were not identified as part of theM. tuberculosiscomplex (MTC) byIS6110PCR, further analysis was conducted using PCR to detect fragments of thehsp65gene.ResultsTwenty-one NTM species were isolated from 230 clinical specimens (14 NTM from pulmonary specimens and 7 from extrapulmonary specimens). Among these, 12 (57.14%) were rapid-growing mycobacteria (RGM) and 9 (42.85%) were slow-growing mycobacteria (SGM). NoM. aviumcomplex (MAC) was identified in any of the specimens. Notably,M. kansasii, M. gordonae, andM. abscessusstrains exhibited significant genetic diversity.ConclusionsThe prevalence of infections attributed to nontuberculous species surpasses that of tuberculosis. These findings underscore the importance of exploring NTM species in individuals suspected of having TB.

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Section: Discussionsupporting

confidence: 91%

Species and Genetic Diversity of Nontuberculous Mycobacteria (NTM) in Suspected Tuberculosis Cases in East Azerbaijan, Iran: A Cross-sectional Analysis

Roshdi Maleki

2024

Preprint

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“…e Zootec. 2023; v30: 1-9. associated with lung diseases, including pulmonary infection (34), pulmonary nodule, necrotic granulomatous pneumonia (35) and pulmonary disease (36,37).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Mycobacteria in Fillets of Nile Tilapia (Oreochromis Niloticus) for Human Consumption

Giorno Eberhardt,

Rodrigues,

Araújo Júnior

et al. 2023

RVZ

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Cinquenta filés de tilápia do Nilo (Oreochromis niloticus) de um mercado de peixes de um município do Estado de São Paulo, Brasil, foram analisados quanto à prevalência de Mycobacterium spp. Após a coleta dos peixes, os filés foram armazenados individualmente e identificados em sacos plásticos estéreis herméticos, colocados em caixa térmica com gelo e levados ao laboratório. Amostras do músculo esquelético (20 mg) foram obtidas e avaliadas por PCR (Polymerase Chain Reaction). Os resultados mostraram a presença de Mycobacterium spp. em 100% das amostras (50/50). O sequenciamento Sanger revelou a presença de Mycobacterium gordonae em todas as amostras. Embora o M. gordonaeseja de baixo potencial patogênico em humanos, a literatura relata infecção em pacientes imunossuprimidos, como pulmões, tecidos moles, córnea, cavidade peritoneal e, menos frequentemente, bacteremia. Assim, o número consideravelmente elevado deste organismo encontrado neste estudo em um prato popular como o filé de tilápia é de grande preocupação. As mudanças nos hábitos alimentares da população, como o aumento do consumo de pescado cru, devem chamar a atenção dos piscicultores e dos comitês de fiscalização, visto que lesões macroscópicas sugestivas geralmente estão ausentes nos produtos. Além disso, é necessário avaliar o papel potencial do Mycobacterium gordonaecomo agente infeccioso quando ingerido através de alimentos, tanto para pessoas imunossuprimidas como para imunocompetentes.

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Investigation of Granulomatous Inflammations in Terms of Tuberculosis Diagnosis: A 5-Year Multi-center Laboratory Study

Oztomurcuk

Terzi

Demirci³

et al. 2022

Turk Thorac J

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Tularemi Lenfadeniti Şüphesi ile Alınan Lenf Aspiratı Örneklerinde Mycobacterium tuberculosis Varlığının Araştırılması

Cited by 3 publications

References 0 publications

Species and Genetic Diversity of Nontuberculous Mycobacteria (NTM) in Suspected Tuberculosis Cases in East Azerbaijan, Iran: A Cross-sectional Analysis

Species and Genetic Diversity of Nontuberculous Mycobacteria (NTM) in Suspected Tuberculosis Cases in East Azerbaijan, Iran: A Cross-sectional Analysis

Molecular Detection of Mycobacteria in Fillets of Nile Tilapia (Oreochromis Niloticus) for Human Consumption

Investigation of Granulomatous Inflammations in Terms of Tuberculosis Diagnosis: A 5-Year Multi-center Laboratory Study

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