Tug of War at the Cell-Matrix Interface

Provenzano, Paolo P.

doi:10.1016/j.bpj.2017.03.032

Cited by 2 publications

(3 citation statements)

References 10 publications

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“…It is therefore crucial to better understand their response to the different stimuli (chemical, topographical, mechanical) provided by their microenvironments. At the cell level, these cues trigger various cellular adaptations concerning adhesion, polarization, migration, or proliferation among others. , Astrocytes were also shown to be sensitive to rigidity, but unlike neurons, they were reported to display better adhesion and a larger spreading area on stiff substrates rather than on soft ones when cultured on laminin or poly- d -lysine coatings. ,,, …”

Section: Introductionmentioning

confidence: 99%

“…At the cell level, these cues trigger various cellular adaptations concerning adhesion, polarization, migration, or proliferation among others. 28,29 Astrocytes were also shown to be sensitive to rigidity, but unlike neurons, they were reported to display better adhesion and a larger spreading area on stiff substrates rather than on soft ones when cultured on laminin or poly-D-lysine coatings. 11,25,26,30 In the present work, we address the influence of the mechanical heterogeneities of the ECM on primary brain cell sensitivity to rigidity in vitro, in dependence with surface chemistry, cell seeding composition, and culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Poly-l-lysine/Laminin Surface Coating Reverses Glial Cell Mechanosensitivity on Stiffness-Patterned Hydrogels

Tomba

Migdal

Fuard

et al. 2022

ACS Appl. Bio Mater.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly-l-lysine/Laminin Surface Coating Reverses Glial Cell Mechanosensitivity on Stiffness-Patterned Hydrogels

Tomba

Migdal

Fuard

et al. 2022

ACS Appl. Bio Mater.

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show abstract

“…It is therefore crucial to better understand their response to the different stimuli (chemical, topographical, mechanical) provided by their microenvironments. Indeed, at the cell level, these cues trigger various cellular adaptations concerning adhesion, polarization, migration or proliferation among others (8,9). For the last two decades, the study of cell response to the mechanical environment has attracted increasing attention (10), both in vivo (11,12) and in vitro (13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Glial cell mechanosensitivity is reversed by adhesion cues

Tomba¹,

Migdal²,

Fuard³

et al. 2019

Preprint

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Brain tissues demonstrate heterogeneous mechanical properties, which evolve with aging and pathologies. The observation in these tissues of smooth to sharp rigidity gradients raises the question of brain cells responses to both different values of rigidity and their spatial variations. Here, we use recent techniques of hydrogel photopolymerization to achieve stiffness structuration down to micrometer resolution. We investigate primary neuron adhesion and orientation as well as glial cell adhesive and proliferative properties on multi-rigidity polyacrylamide hydrogels presenting a uniform density of adhesive molecules. We first observed that neurons grow following rigidity gradients. Then, our main observation is that glial cell adhesion and proliferation can be enhanced on stiff or on soft regions depending on the adhesive coating of the hydrogel, i. e. fibronectin or poly-L-lysine/laminin. This behavior was unchanged in the presence or not of neuronal cells. In addition, and contrarily to other cell types, glial cells were not confined by sharp, micron-scaled gradients of rigidity. Our observations suggest that their mechanosensitivity could involve adheison-related mechanosensitive pathways that are specific to brain tissues. SIGNIFICANCE By growing primary brain cells on 2D multi-rigidity polyacrylamide hydrogels, we show that favorable culture conditions for glial cells switch from stiff to soft substrates when changing the adhesive ligands from fibronectin to poly-L-lysine/laminin. Together with neurons, glial cells thus provide a unique example where soft is preferred to stiff, but unlike neurons, this preference can be reversed by changing the nature of the coating. We additionally show that contrarily to other cell types, glial cells are deformed by subcellular gradients of rigidity but cannot be confined by these rigidity gradients. These observations point that glial cell use a very specific, integrin-related machinery for rigidity sensing.

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Tug of War at the Cell-Matrix Interface

Cited by 2 publications

References 10 publications

Poly-l-lysine/Laminin Surface Coating Reverses Glial Cell Mechanosensitivity on Stiffness-Patterned Hydrogels

Poly-l-lysine/Laminin Surface Coating Reverses Glial Cell Mechanosensitivity on Stiffness-Patterned Hydrogels

Glial cell mechanosensitivity is reversed by adhesion cues

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