Tuftsin-derived T-peptide prevents cellular immunosuppression and improves survival rate in septic mice

Gao, Yulei; Chai, Yifeng; Dong, Ning; Han, Shuang; Zhu, Xiaomei; Zhang, Qinghong; Yao, Yong‐Ming

doi:10.1038/srep16725

Cited by 15 publications

(27 citation statements)

References 51 publications

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“…We have reported that the percentage and stability of Tregs were higher in septic patients than those without sepsis; a reduction in the stability of Tregs was accompanied by an improvement in survival rate and immune dysfunction of T-lymphocytes with sepsis [12–15, 23, 24]. Foxp- 3, which is still the mainly intracellular marker for identification of Tregs, was also critical for their function, differentiation, and maintenance [10, 11].…”

Section: Discussionmentioning

confidence: 99%

“…CD4 + CD25 + Tregs and CD4 + CD25 − T cells were isolated using mouse CD4 + CD25 + Tregs isolation kits, which we have recounted [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

“…The classical septic model, that is, CLP, is used which we have recounted in our previous studies to induce sepsis [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

“…The expression levels of Foxp-3, CTLA-4, and TGF- β m+ of CD4 + CD25 + Tregs, as well as the apoptotic rates of CD4 + CD25 + Tregs and CD4 + CD25 − T cells, were determined by flow cytometer (Becton-Dickinson), which we have recounted [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

“…The proliferative ability of CD4 + CD25 − T cells was determined by CCK-8, which we have recounted [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

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The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

Gao

Lü

Zhai

et al. 2017

Mediators of Inflammation

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Cellular immunosuppression appears to be involved in sepsis and sepsis-induced multiple organ dysfunction syndrome (MODS). Recent evidence showed that parenteral vitamin C (Vit C) had the ability to attenuate sepsis and sepsis-induced MODS. Herein, we investigated the impact of parenteral Vit C on cellular immunosuppression and the therapeutic value in sepsis. Using cecal ligation and puncture (CLP), sepsis was induced in WT and Gulo−/− mice followed with 200 mg/Kg parenteral Vit C administration. The immunologic functions of CD4+CD25+ regulatory T cells (Tregs) and CD4+CD25− T cells, as well as the organ functions, were determined. Administration of parenteral Vit C per se markedly improved the outcome of sepsis and sepsis-induced MODS of WT and Gulo−/− mice. The negative immunoregulation of Tregs was inhibited, mainly including inhibiting the expression of forkhead helix transcription factor- (Foxp-) 3, cytotoxic T lymphocyte associated antigen- (CTLA-) 4, membrane associated transforming growth factor-β (TGF-βm+), and the secretion of inhibitory cytokines [including TGF-β and interleukin- (IL-) 10], as well as CD4+ T cells-mediated cellular immunosuppression which was improved by parenteral Vit C in WT and Gulo−/− septic mice. These results suggested that parenteral Vit C has the ability to improve the outcome of sepsis and sepsis-induced MODS and is associated with improvement in cellular immunosuppression.

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Section: Discussionmentioning

confidence: 99%

“…CD4 + CD25 + Tregs and CD4 + CD25 − T cells were isolated using mouse CD4 + CD25 + Tregs isolation kits, which we have recounted [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

“…The classical septic model, that is, CLP, is used which we have recounted in our previous studies to induce sepsis [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…The proliferative ability of CD4 + CD25 − T cells was determined by CCK-8, which we have recounted [23, 24]. …”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

Gao

Lü

Zhai

et al. 2017

Mediators of Inflammation

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Coupling Surface Plasmon Polariton Modes to Complementary THz Metasurfaces Tuned by Inter Meta‐Atom Distance

Keller

Maissen

Haase

et al. 2017

Advanced Optical Materials

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The authors study the interaction of complementary terahertz (THz) split ring resonators with THz surface plasmon polaritons (SPPs) as a function of the meta-atom distance. The THz transmission properties of 15 samples for which the array dimensions are varied keeping the resonator shape constant are investigated. The linewidth of the inductive-capacitive (LC-)resonance is decreasing with increasing meta-atom distance, up to the frequency matching with the first SPP-mode. The SPP-mode couples to the narrow LC-resonance leading to an anti-crossing of the modes. In contrast, the narrow SPP-mode tunes across the broader dipole-like mode in orthogonal polarization. The excitation direction of the SPP-mode is found to lie along the electric field polarization of the THz. www.advopticalmat.de Figure 5. a) Normalized transmission spectra as a function of the lattice constant with a x = a y for the broad dipole-mode excited in x-direction. The calculated SPP-modes that cross the broad dipole-mode are traced in blue solid lines after Equation (2). Black sections denote not sampled lattice constants. The normalized transmission of the dipole-mode as function of the frequency is shown for square lattices in b) for a x = a y = 70 µm and c) for a x = a y = 85 µm. Transmission spectra of rectangular lattices are shown for d) a x = 70 µm and a y = 85 µm and for e) a x = 85 µm and a y = 70 µm.

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The contribution of neuropilin‐1 in the stability of CD4⁺CD25⁺ regulatory T cells through the TGF‐β1/Smads signaling pathway in the presence of lipopolysaccharides

Gao

Zhang

Wang

et al. 2021

Immunity Inflam & Disease

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Introduction: This study investigates the synergistic effect of TGF-β1 and Nrp-1 on CD4 + CD25 + T regs ' stabilization, and the associated pathways of signal transduction, in vitro models in the presence of LPS.Materials and Methods: Spleen CD4 + CD25 + T regs cells of mice models in the presence of LPS, were transfected with an shRNA targeting Nrp-1, Smad2, or Smad3, may or may not be treated with recombinant TGF-β1. Followed by subsequent determination of cellular proliferation, rate of apoptosis, observation of the Foxp3, CTLA-4, and TGF-β1 m+ expression levels, foxp3-TSDR methylation, secretion levels of the inhibitory cytokines IL-10 and TGF-β1, and Smad2/3 of CD4 + CD25 + T regs expression.Results: A remarkable stimulation in CD4 + CD25 + T regs ' stability is noted after administering recombinant TGF-β1 in the presence of LPS, and promoted cellular viability, increased Foxp3, CTLA-4, and TGF-β1 m+ expression, and elevated secretion of IL-10 and TGF-β1. This also inhibited the apoptosis and methylation of foxp3-TSDR of CD4 + CD25 + T regs . The shRNA transfection silenced Nrp-1 and Smad3, but not Smad2, resulting in the suppression of the recombinant TGF-β1-mediated effects in the presence of LPS.Conclusions: According to the results, Nrp-1 mediates TGF-β1 to improve the stability of regulatory CD4 + CD25 + T cells and maybe a possible therapeutic target with the ability to improve the CD4 + CD25 + T regs associated negative immunoregulation that is related to the TGF-β1/Smads cell signaling during sepsis.

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Tuftsin-derived T-peptide prevents cellular immunosuppression and improves survival rate in septic mice

Cited by 15 publications

References 51 publications

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

Coupling Surface Plasmon Polariton Modes to Complementary THz Metasurfaces Tuned by Inter Meta‐Atom Distance

The contribution of neuropilin‐1 in the stability of CD4⁺CD25⁺ regulatory T cells through the TGF‐β1/Smads signaling pathway in the presence of lipopolysaccharides

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Tuftsin-derived T-peptide prevents cellular immunosuppression and improves survival rate in septic mice

Cited by 15 publications

References 51 publications

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

The Parenteral Vitamin C Improves Sepsis and Sepsis-Induced Multiple Organ Dysfunction Syndrome via Preventing Cellular Immunosuppression

Coupling Surface Plasmon Polariton Modes to Complementary THz Metasurfaces Tuned by Inter Meta‐Atom Distance

The contribution of neuropilin‐1 in the stability of CD4+CD25+ regulatory T cells through the TGF‐β1/Smads signaling pathway in the presence of lipopolysaccharides

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The contribution of neuropilin‐1 in the stability of CD4⁺CD25⁺ regulatory T cells through the TGF‐β1/Smads signaling pathway in the presence of lipopolysaccharides