Tuftelin Is Required for NGF-Induced Differentiation of PC12 Cells

Shilo, Dekel; Cohen, Gal; Blumenfeld, Anat; Goren, Koby; Hanhan, Salem; Sharon, Shay; Haze, Amir; Deutsch, D.; Lazarovici, Philip

doi:10.1007/s12031-019-01292-1

Cited by 10 publications

(4 citation statements)

References 32 publications

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“…Rat pheochromocytoma (PC12) cell is an ideal cell for studying the physiology, pathology, and pharmacology of nerve cells. The cell membrane has nerve growth factor (NGF) receptors, which can be transformed into neuron‐like cells with neuronal characteristics after NGF‐induced stimulation (Shilo et al., 2019; Xiong Gao et al., 2021). For example, PC12 cells have significantly elongated and intertwined processes, presenting a typical neuronal morphology; cells are anti‐nerve Metadifferentiation marker microtubule‐associated protein 2 staining was strongly positive.…”

Section: Ad Cell Modelmentioning

confidence: 99%

Nutritional assessment models for Alzheimer's disease: Advances and perspectives

Wen

Zhang

et al. 2023

Food Frontiers

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Alzheimer's disease (AD) is a neurodegenerative disease of uncertain pathogenesis that develops in the elderly population and is characterized by loss of cognitive function, memory loss, and deterioration of everyday behavior. The development of biological models of AD is constrained by the complexity of the pathogenesis and multiple causes. Creating disease models can aid in understanding the pathophysiology of AD and developing effective therapeutic drugs. In this review, the animal and cellular models that are currently being utilized to research AD are outlined and described in detail according to modeling approaches. The main animal models are natural aging models, transgenic models, and drug‐induced models. The main cellular models are traditional 2D cells, human induced pluripotent stem 2D cells (neurons and glial cells), and 3D cells, as well as organoid models, and the benefits and drawbacks of their various biological models are assessed. They do have their limitations due to an incomplete reflection of AD pathology. Therefore, new models for AD research are needed in the future. The summary of existing models is intended to provide a basis for the subsequent development of new disease models and to provide a reference for the study of disease mechanisms, clinical research, and new drug development in AD.

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Section: Ad Cell Modelmentioning

confidence: 99%

Nutritional assessment models for Alzheimer's disease: Advances and perspectives

Wen

Zhang

et al. 2023

Food Frontiers

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“…Tuftelin 1 (TUFT1) is an enamel associated teeth protein deemed to play a role in enamel mineralization. As well as, TUFT1 is expressed in embryonic stem cells, neuronal cells, and other non‐mineralizing tissues like brain, eyes, lung, adrenal gland, liver, kidneys, testes, and tumor tissues 8,9 . Oncogenic TUFT1 was associated with HCC progression and could be a useful molecular‐target for inhibiting HCC growth 10 .…”

Section: Introductionmentioning

confidence: 99%

“…As well as, TUFT1 is expressed in embryonic stem cells, neuronal cells, and other non-mineralizing tissues like brain, eyes, lung, adrenal gland, liver, kidneys, testes, and tumor tissues. 8,9 Oncogenic TUFT1 was associated with HCC progression and could be a useful molecular-target for inhibiting HCC growth. 10 Recently, it has been demonstrated for the first time that the expression of both TUFT 1-mRNA, and protein was upregulated in HCC cells.…”

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confidence: 99%

The expression of tuftelin 1 as a new theranostic marker in early diagnosis and as a therapeutic target in hepatocellular carcinoma

Abdel-Hamid

Zakaria

Ansary

et al. 2023

Cell Biochemistry & Function

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Currently, many challenges are associated with hepatocellular carcinoma (HCC) as the failure of early diagnosis, and the lack of effective therapy. This study aimed to investigate the possible role of tuftelin 1 (TUFT 1) in the early diagnosis of HCC and evaluate the potential contribution of the TUFT 1/Ca+2/phosphinositol 3 kinase (PI3K) pathway in dantrolene sodium (Dan) therapeutic outcomes. The study was performed on two sets of rats, the staging (30 rats) and treatment sets (80 rats). HCC was induced by a single dose of diethylnitrosamine (DENA). The hepatic content of TUFT 1 protein was assayed via western blot and immunohistochemistry (IHC), while PI3K, vascular endothelial growth factor (VEGF), Cyclin D1, and matrix‐metalloproteinase‐9 (MMP‐9) contents were assessed using enzyme‐linked immunosorbent assay. Hepatic and serum calcium were measured colorimetrically. Furthermore, the nuclear proliferation marker, (Ki‐67), (Kiel [Ki] where the antibody was produced in the University Department of Pathology and the original clone number is 67)—expression was assessed by IHC. TUFT 1/Ca+2/PI3K signaling pathway was progressively activated in the 3 studied stages of HCC with subsequent upregulation of angiogenesis, cell cycle, and metastasis. More interestingly, Dan led to TUFT 1/Ca+2/PI3K pathway disruption by diminution of the hepatic contents of TUFT 1, calcium, PI3K, VEGF, Cyclin D1, and MMP‐9 in a dose‐dependent pattern. TUFT 1 can serve as a theranostic biomarker in HCC. Moreover, Dan exerted an antineoplastic effect against HCC via the interruption of TUFT 1/Ca+2/PI3K pathway.

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“…TUFT1 belongs to the enamel associated teeth proteins and is thought to play a role in enamel mineralization. Also, TUFT1 is expressed in embryonic stem cells, neuronal cells, and non-mineralizing tissues like eyes, brain, adrenal gland, lung, liver, kidneys, testis, and tumor tissues[ 13 - 15 ]. TUFT1 can modulate the Rab GTPase-regulated process to activate the mammalian target of rapamycin complex 1 signaling that is activated in many cancers[ 16 ] and associated with poor prognosis in breast or pancreatic cancer and thyroid carcinoma[ 10 , 11 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

Wu¹,

Zheng²,

Ye³

et al. 2021

WJG

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BACKGROUND Abnormal tuftelin 1 (TUFT1) has been reported in multiple cancers and exhibits oncogenic roles in tumor progression. However, limited data are available on the relationship between TUFT1 and hepatocellular carcinoma (HCC), and the exact biological mechanism of TUFT1 is still poorly understood in HCC. AIM To investigate TUFT1 expression in HCC and how interfering TUFT1 transcription affects HCC growth. METHODS TUFT1 in HCC and non-HCC tissues based on databases of the Cancer Genome Atlas and Oncomine were analyzed, and TUFT1 in human HCC tissues on microarray were detected by immunohistochemistry for clinicopathological features, overall survival, and disease-free survival. HCC cells were transfected with constructed vectors of TUFT1 that interfere or over-express TUFT1 for analyzing the biological behaviors of HCC cells. Proliferation, invasion, migration, and apoptosis of cells were detected by cell counting kit-8, scratch assay, transwell tests, and flow cytometry and confirmed by Western blotting, respectively. RESULTS Abnormal TUFT1 levels in databases expressed in HCC at messenger RNA (mRNA) level and HCC tissues were mainly located in cytoplasm and membrane. The level of TUFT1 expression in the HCC group was significantly higher ( χ 2 = 18.563, P < 0.001) than that in the non-cancerous group, closely related to clinical staging, size, vascular invasion of tumor, hepatitis B e-antigen positive, and ascites ( P < 0.01) of HCC patients, and negatively to HCC patients’ overall survival and disease-free survival ( P < 0.001). After interfering with TUFT1 transcription at mRNA level in the MHCC-97H cells by the specific TUFT1-short hairpin RNA, cell proliferation, invasion, and metastasis were significantly inhibited with increasing apoptosis rate. In contrast, proliferation, invasion, and migration were significantly enhanced after over-expression of TUFT1 mRNA in Hep3B cells in vitro . CONCLUSION Oncogenic TUFT1 was associated with the progression of HCC and could be a potential molecular-target for inhibiting HCC growth.

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Tuftelin Is Required for NGF-Induced Differentiation of PC12 Cells

Cited by 10 publications

References 32 publications

Nutritional assessment models for Alzheimer's disease: Advances and perspectives

Nutritional assessment models for Alzheimer's disease: Advances and perspectives

The expression of tuftelin 1 as a new theranostic marker in early diagnosis and as a therapeutic target in hepatocellular carcinoma

Oncogenic tuftelin 1 as a potential molecular-targeted for inhibiting hepatocellular carcinoma growth

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