Tudor domain‐containing proteins of <i>Drosophila melanogaster</i>

Ying, Muying; Chen, Dahua

doi:10.1111/j.1440-169x.2011.01308.x

Cited by 27 publications

(20 citation statements)

References 62 publications

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“…In mammals, 12 members of the TDRD protein family have been identified since Drosophila Tudor proteins were discovered [18,20,22,23,24,29,30,36,37,38]. We also demonstrated that our newly identified Tdrd12 is most homologous to mouse and human TDRD12 among the 12 TDRD family members.…”

Section: Discussionsupporting

confidence: 59%

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Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish

Dai

Shu

Lou

et al. 2017

IJMS

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The regularity of Piwi-interacting RNA (piRNA) biogenesis is crucial to germline development. Functioning as Piwi-interacting proteins, Tudor domain-related proteins (Tdrds) have been demonstrated to be involved in spermatogenesis and the piRNA pathway. In this study, zebrafish tdrd12 was identified, and the maternal and germ cell-specific expression patterns of zebrafish tdrd12 were observed. Utilizing TALEN (transcription activator-like effector nuclease) techniques, two independent tdrd12 mutant zebrafish lines were generated. Although no defects were found during the generation of the primordial germ cells (PGCs) in the tdrd12-null fish progenies obtained from the heterozygous tdrd12 mutant parents, all Tdrd12-deficient fish developed into infertile males. The reduced numbers and eventually loss of the germ cells by 35 days post fertilization (dpf) led to masculinization and infertility of the Tdrd12-deficient fish. Meiosis defects of the germ cells in the tdrd12 mutants during the gonad-transitioning period were observed, revealing the indispensable functions of Tdrd12 in gametogenesis. Our studies demonstrated that zebrafish Tdrd12 is essential for germ cell development and maintenance.

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Section: Discussionsupporting

confidence: 59%

“…Among PIWI-interacting proteins, Tdrd family proteins have been revealed as important molecules for piRNA biogenesis. Twelve members of TDRD family proteins have been identified in mice since the Drosophila Tudor protein was discovered [20,21]. They are mainly involved in germ cell development [19].…”

Section: Introductionmentioning

confidence: 99%

Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish

Dai

Shu

Lou

et al. 2017

IJMS

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“…tudor is one of 23 Tudor domain-containing proteins in Drosophila (Ying and Chen, 2012), but there is no evidence that loss of function of other Tudor domain-containing genes have grandchildless phenotypes in Drosophila (Handler et al, 2011; Pek et al, 2012). We therefore focus only on the expression and function of the orthologue of Drosophila tudor (CG9450).…”

Section: Resultsmentioning

confidence: 99%

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

2013

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SummaryPrimordial germ cell (PGC) formation in holometabolous insects like Drosophila melanogaster relies on maternally synthesised germ cell determinants that are asymmetrically localised to the oocyte posterior cortex. Embryonic nuclei that inherit this “germ plasm” acquire PGC fate. In contrast, historical studies of basally branching insects (Hemimetabola) suggest that a maternal requirement for germ line genes in PGC specification may be a derived character confined principally to Holometabola. However, there have been remarkably few investigations of germ line gene expression and function in hemimetabolous insects. Here we characterise PGC formation in the milkweed bug Oncopeltus fasciatus, a member of the sister group to Holometabola, thus providing an important evolutionary comparison to members of this clade. We examine the transcript distribution of orthologues of 19 Drosophila germ cell and/or germ plasm marker genes, and show that none of them localise asymmetrically within Oncopeltus oocytes or early embryos. Using multiple molecular and cytological criteria, we provide evidence that PGCs form after cellularisation at the site of gastrulation. Functional studies of vasa and tudor reveal that these genes are not required for germ cell formation, but that vasa is required in adult males for spermatogenesis. Taken together, our results provide evidence that Oncopeltus germ cells may form in the absence of germ plasm, consistent with the hypothesis that germ plasm is a derived strategy of germ cell specification in insects.

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“…These genes coded for proteins with regulative TUDOR, NYN or BTB/POZ domains (Anantharaman & Aravind ; Misra et al . ; Ying & Chen ). We found that members of these putative regulatory families were constitutively downregulated upon adaptation (Table S2, Supporting information).…”

Section: Discussionmentioning

confidence: 99%

Adaptation of a polyphagous herbivore to a novel host plant extensively shapes the transcriptome of herbivore and host

et al. 2015

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Generalist arthropod herbivores rapidly adapt to a broad range of host plants. However, the extent of transcriptional reprogramming in the herbivore and its hosts associated with adaptation remains poorly understood. Using the spider mite Tetranychus urticae and tomato as models with available genomic resources, we investigated the reciprocal genomewide transcriptional changes in both spider mite and tomato as a consequence of mite's adaptation to tomato. We transferred a genetically diverse mite population from bean to tomato where triplicated populations were allowed to propagate for 30 generations. Evolving populations greatly increased their reproductive performance on tomato relative to their progenitors when reared under identical conditions, indicative of genetic adaptation. Analysis of transcriptional changes associated with mite adaptation to tomato revealed two main components. First, adaptation resulted in a set of mite genes that were constitutively downregulated, independently of the host. These genes were mostly of an unknown function. Second, adapted mites mounted an altered transcriptional response that had greater amplitude of changes when re-exposed to tomato, relative to nonadapted mites. This gene set was enriched in genes encoding detoxifying enzymes and xenobiotic transporters. Besides the direct effects on mite gene expression, adaptation also indirectly affected the tomato transcriptional responses, which were attenuated upon feeding of adapted mites, relative to the induced responses by nonadapted mite feeding. Thus, constitutive downregulation and increased transcriptional plasticity of genes in a herbivore may play a central role in adaptation to host plants, leading to both a higher detoxification potential and reduced production of plant defence compounds.

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Tudor domain‐containing proteins of Drosophila melanogaster

Cited by 27 publications

References 62 publications

Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish

Tdrd12 Is Essential for Germ Cell Development and Maintenance in Zebrafish

Evidence against a germ plasm in the milkweed bugOncopeltus fasciatus, a hemimetabolous insect

Adaptation of a polyphagous herbivore to a novel host plant extensively shapes the transcriptome of herbivore and host

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