Tubulin marker line of grapevine suspension cells as a tool to follow early stress responses

Guan, Xin; Buchholz, Günther; Nick, Peter

doi:10.1016/j.jplph.2014.10.023

Cited by 16 publications

(16 citation statements)

References 63 publications

(53 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A suspension cell culture of Vitis rupestris expressing the fluorescent tubulin marker GFP‐AtTUB6 (Guan et al, ) was used in this experiment. The cells were cultivated in liquid medium containing 4.3 g‐L −1 Murashige and Skoog salts (Duchefa, Haarlem, The Netherlands), 30 g‐L −1 sucrose, 200 mg‐L −1 KH 2 PO 4 , 100 mg‐L −1 inositol, 1 mg‐L −1 thiamine, and 0.2 mg‐L −1 2,4‐dichlorophenoxyacetic acid (2,4‐D), pH 5.8, and 35 mg‐L −1 hygromycin.…”

Section: Methodsmentioning

confidence: 99%

“…The response of central and cortical MTs was followed over time using the GFP‐AtTuB6 marker (Guan et al, ) after application of cold stress (0 °C) and the different chemical treatments in individual cells by spinning‐disc confocal microscopy. Confocal images were recorded with an AxioObserver Z1 (Zeiss, Jena, Germany) using a 63 × LCI‐NeofluarImmCorr DIC objective (NA 1.3), the 488‐nm emission line of an Ar‐Kr laser, and a spinning‐disc device (YOKOGAWA CSU‐X1 5000).…”

Section: Methodsmentioning

confidence: 99%

“…Information about the events and molecular components that convey cold signalling from stimulus to perception, and then further from perception to early signal transduction culminating in cold adaptation is central to identify genetic factors that promote cold hardiness. Using a transgenic grapevine cell line expressing a fluorescent tubulin marker (Guan, Buchholz, & Nick, ), we dissect the early events of cold signalling upstream of MTs. We show that calcium influx, membrane rigidification, and activation of NAD(P)H oxidase contribute to signalling and that a G protein in concert with PLD convey the signal towards MTs, whereas calmodulin seems to be not involved.…”

Section: Introductionmentioning

confidence: 99%

“…Alkalinization was followed over 1 hr, and peak values (corrected for the basal value established during pre-equilibration) reached at around 35 min after the onset of treatment were plotted relative to the value in the control (treatment with 0°C without addition of any compounds). In addition to this ice water control without any chemical treatment, a second control kept at room temperature (27°C) was included-in this second control, no fluctuation of apoplastic pH was observed.2.7 | Microscopy and image processingThe response of central and cortical MTs was followed over time using the GFP-AtTuB6 marker(Guan et al, 2015) after application of cold stress (0°C) and the different chemical treatments in individual cells by spinning-disc confocal microscopy. Confocal images were recorded with an AxioObserver Z1 (Zeiss, Jena, Germany) using a 63 × LCI-NeofluarImmCorr DIC objective(NA 1.3), the 488-nm emission line of an Ar-Kr laser, and a spinning-disc device (YOKOGAWA CSU-X1 5000).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Cold sensing in grapevine—Which signals are upstream of the microtubular “thermometer”

Wang

Nick

2017

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Plants can acquire freezing tolerance in response to cold but non-freezing temperatures. To efficiently activate this cold acclimation, low temperature has to be sensed and processed swiftly, a process that is linked with a transient elimination of microtubules. Here, we address cold-induced microtubules elimination in a grapevine cell line stably expressing a green fluorescent protein fusion of Arabidopsis TuB6, which allows to follow their response in vivo and to quantify this response by quantitative image analysis. We use time-course studies with several specific pharmacological inhibitors and activators to dissect the signalling events acting upstream of microtubules elimination. We find that microtubules disappear within 30 min after the onset of cold stress. We provide evidence for roles of calcium influx, membrane rigidification, and activation of NAD(P)H oxidase as factors in signal susception and amplification. We further conclude that a G-protein in concert with a phospholipase D convey the signal towards microtubules, whereas calmodulin seems to be not involved. Moreover, activation of jasmonate pathway in response to cold is required for an efficient microtubule response. We summarize our findings in a working model on a complex signalling hub at the membrane-cytoskeleton interphase that assembles the susception, perception and early transduction of cold signals.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Cold sensing in grapevine—Which signals are upstream of the microtubular “thermometer”

Wang

Nick

2017

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

show abstract

“…We found that the G. arboreum EST JK757657 was homolog of Arabidopsis (AT1G29830), a microtubule (dyenin protein). Plant microtubules (PMT's), dynein have major role in in cell division and expansion, sensory transmission particularly pertinent to membrane stress and its derivatives, such as cold and water stress (Nick, 2013;Guan et al, 2015). MT's has been significantly known for their roles in sensing of osmotic stress (Wang et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)

Jamal¹,

Shahid²,

Aftab³

et al. 2019

PAK.J.BOT.

View full text Add to dashboard Cite

Cotton is major fiber crop among agricultural commodities grown worldwide. Prolonged osmotic stress and seasonal water shortage effect plant growth and yields. To explore the drought responsive molecular mechanism in cotton roots, we constructed cDNA library for the identification, functional annotation and expression analysis of potential drought responsive transcripts. mRNA extracted from cotton roots under drought stress was used to construct cDNA library. A total of 711 expressed sequence tags were generated from cDNA library. NCBI BLAST analysis of reported expressed sequence tags showed homology of 27% sequences with Gossypium species while 24% sequences showed no sequence similarity in non-redundant nucleotide database. Remaining 49% sequences revealed sequence similarity results with other plant species. Functional annotation was performed for sequenced EST's using Arabidopsis Gene ontology database (TAIR). We examined the expression pattern of 08 differentially expressed ESTs using RT-qPCR and validated that all these were upregulated in roots under osmotic stress, but to different folds. The candidate EST sequences identified in this study will be valuable resource to elucidate stress responsive molecular mechanism and genetic breeding of cotton in future.

show abstract

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

et al. 2022

Self Cite

View full text Add to dashboard Cite

Key message Paclitaxel synthesis in Taxus cells correlates with a cell-fate switch that leads to vacuoles of a glossy appearance and vermiform mitochondria. This switch depends on actin and apoplastic respiratory burst. Abstract Plant cell fermentation, the production of valuable products in plant cell culture, has great potential as sustainable alternative to the exploitation of natural resources for compounds of pharmaceutical interest. However, the success of this approach has remained limited, because the cellular aspects of metabolic competence are mostly unknown. The production of the anti-cancer alkaloid Paclitaxel has been, so far, the most successful case for this approach. In the current work, we map cellular aspects of alkaloid synthesis in cells of Taxus chinensis using a combination of live-cell imaging, quantitative physiology, and metabolite analysis. We show evidence that metabolic potency correlates with a differentiation event giving rise to cells with large vacuoles with a tonoplast that is of a glossy appearance, agglomerations of lipophilic compounds, and multivesicular bodies that fuse with the plasma membrane. Cellular features of these glossy cells are bundled actin, more numerous peroxisomes, and vermiform mitochondria. The incidence of glossy cells can be increased by aluminium ions, and this increase is significantly reduced by the actin inhibitor Latrunculin B, and by diphenylene iodonium, a specific inhibitor of the NADPH oxidase Respiratory burst oxidase Homologue (RboH). It is also reduced by the artificial auxin Picloram. This cellular fingerprint matches the implications of a model, where the differentiation into the glossy cell type is regulated by the actin–auxin oscillator that in plant cells acts as dynamic switch between growth and defence.

show abstract

Tubulin marker line of grapevine suspension cells as a tool to follow early stress responses

Cited by 16 publications

References 63 publications

Cold sensing in grapevine—Which signals are upstream of the microtubular “thermometer”

Cold sensing in grapevine—Which signals are upstream of the microtubular “thermometer”

Generation and analysis of expressed sequence tags from roots cDNA library of cotton (Gossypium arboreum)

Switching cell fate by the actin–auxin oscillator in Taxus: cellular aspects of plant cell fermentation

Contact Info

Product

Resources

About