Tubulin isoform usage in maize microtubules

Eun, Soon-Ok; Wick, Susan M.

doi:10.1007/bf01280327

Cited by 12 publications

(8 citation statements)

References 56 publications

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“…The same could be true for MAP65-9, which is specific to stamens and pollen. This phenomenon has also been seen in the case of maize b-tubulins, where b2-tubulin is transcribed in many tissues but utilized only in male meiocytes (Eun and Wick, 1998). (3) The amount of protein is below the sensitivity of the immunofluorescence technique.…”

Section: Differential Localization Of At Map65 Isotypesmentioning

confidence: 89%

The C-Terminal Variable Region Specifies the Dynamic Properties ofArabidopsisMicrotubule-Associated Protein MAP65 Isotypes

et al. 2008

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The microtubule-associated protein, MAP65, is a member of a family of divergent microtubule-associated proteins from different organisms generally involved in maintaining the integrity of the central spindle in mitosis. The dicotyledon Arabidopsis thaliana and the monocotyledon rice (Oryza sativa) genomes contain 9 and 11 MAP65 genes, respectively. In this work, we show that the majority of these proteins fall into five phylogenetic clades, with the greatest variation between clades being in the C-terminal random coil domain. At least one Arabidopsis and one rice isotype is within each clade, indicating a functional specification for the C terminus. In At MAP65-1, the C-terminal domain is a microtubule binding region (MTB2) harboring the phosphorylation sites that control its activity. The At MAP65 isotypes show differential localization to microtubule arrays and promote microtubule polymerization with variable efficiency in a MTB2-dependent manner. In vivo studies demonstrate that the dynamics of the association and dissociation of different MAP65 isotypes with microtubules can vary up to 10-fold and that this correlates with their ability to promote microtubule polymerization. Our data demonstrate that the C-terminal variable region, MTB2, determines the dynamic properties of individual isotypes and suggest that slower turnover is conditional for more efficient microtubule polymerization.

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Section: Differential Localization Of At Map65 Isotypesmentioning

confidence: 89%

The C-Terminal Variable Region Specifies the Dynamic Properties ofArabidopsisMicrotubule-Associated Protein MAP65 Isotypes

et al. 2008

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“…Procedures were based on the method described by Eun and Wick (1998) and modified for better labeling of microtubules. At the end of lead treatment, apical 5-mm segments of primary roots were excised under a fixative containing 3.8% (w/v) paraformaldehyde, 10% (v/v) dimethyl sulfoxide, 0.1% (v/v) Triton X-100, 5 mM MgCl 2 and 5 mM EGTA prepared in 50 mM phosphate buffer.…”

Section: Fixation and Cryosectioning For Labeling Microtubulesmentioning

confidence: 99%

Lead disturbs microtubule organization in the root meristem of Zea mays

Eun¹,

Youn²,

Lee³

2000

Physiol Plant

141

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effects of lead on microtubules in the root meristem. LeadLead is an environmental pollutant that interferes with plant growth. Unfortunately, the mechanisms of lead toxicity in treatment perturbed the alignment of microtubules in a concentration-dependent manner beginning at 10 mM. Microtubules of plants are still poorly understood. In this study, we have investigated both the deposition sites and sources of cellular different regions of the root meristem and in different stages of the cell cycle showed differential susceptibility to lead. These toxicity of lead in maize seedlings (Zea mays L. cv. Golden Cross Bantam). Using atomic absorption spectroscopy and effects do not appear to be general phenomena common to toxic metals, since aluminum and copper, at concentrations that X-ray fluorescence microprobing, we show that lead accumulation is highest in the root meristem, and that the accumulation decreased root growth to a comparable level, did not have the same detrimental effects on microtubules. Based on these occurs both in the apoplast and symplast. Since cells are results, we suggest that the damage to microtubules is partly dividing vigorously in this region and because microtubules play responsible for lead-associated toxicity in plants. an important role in cell division, we have further examined the various pollutants, including heavy metals (Ma et al. 1995, Panda et al. 1997, Kovalchuk et al. 1998, Steinkellner et al. 1998. Because formation of multiple micronuclei results from improper nuclear separation and cytokinetic disorder, we suspect that the components of the processes may be directly susceptible to toxic metals. Microtubules play key roles in both nuclear division and cytokinesis in plants. Thus, in the present work, we examined lead deposition sites in seedlings of maize and the effects of lead on the microtubule organization in the region of root where lead deposition was the highest. Materials and methods Plant materialMaize (Zea mays L. cv. Golden Cross Bantam) seeds were sterilized for 15 min with 0.5% calcium hypochlorite, washed several times with tap water and soaked overnight in

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“…The expression of certain a-tubulin genes is tissue specific in maize (Montoliu et al 1989;Joyce et al 1992;Uribe et al 1998), as well in other plants (Ludwig et al 1988;Carpenter et al 1992;Kopczak et al 1992;Dixon et al 1994;Hellmann and Wernicke 1998;Schro¨der et al 2001). Immunofluorescence microscopy showed that all the isoforms of maize a-and b-tubulins examined appear to be incorporated into MTs to various extents (Eun and Wick 1998).…”

Section: Introductionmentioning

confidence: 99%

Post-translational modifications of ?-tubulin in Zea mays L. are highly tissue specific

Wang

Vignani

Scali

et al. 2004

Planta

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To further understand post-translational modifications (PTMs) of plant alpha-tubulin, post-translationally modified alpha-tubulin isoforms from selected tissues of Zea mays L. were examined using two-dimensional electrophoresis and immunoblotting. Except for polyglycylated tubulin, tyrosinated, detyrosinated, acetylated and polyglutamylated alpha-tubulin isoforms were all present in maize tissues. Tyrosinated alpha-tubulin was the predominant variant in all cases, with isoforms alpha1-alpha4 (alpha5) being the most common components. Leaves exhibited a striking difference in PTM patterns of alpha-tubulin isoforms compared to other tissues examined. In leaves, several major specific isoforms were highly modified by detyrosination, acetylation and polyglutamylation. In pollen and anthers, only the most abundant isoform alpha3 was acetylated to an appreciable extent, and no acetylated isoform was found in roots. Similarly, in pollen, anthers and roots, only alpha3 was appreciably polyglutamylated. Additionally, a detyrosinated isoform alpha6 was present in anthers and in leaves, while the tyrosinated isoform alpha6 seemed to be pollen specific. These results indicate that certain types of PTM of plant alpha-tubulin preferentially occur in a tissue-specific way.

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Tubulin isoform usage in maize microtubules

Cited by 12 publications

References 56 publications

The C-Terminal Variable Region Specifies the Dynamic Properties ofArabidopsisMicrotubule-Associated Protein MAP65 Isotypes

The C-Terminal Variable Region Specifies the Dynamic Properties ofArabidopsisMicrotubule-Associated Protein MAP65 Isotypes

Lead disturbs microtubule organization in the root meristem of Zea mays

Post-translational modifications of ?-tubulin in Zea mays L. are highly tissue specific

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