Tubular secretion of Tamm-Horsfall protein in type 1 (insulin-dependent) diabetes mellitus using a simplified enzyme linked immunoassay

Torffvit, Ole; Agardh, Carl‐David; Kjellsson, B.; Wieslander, J.

doi:10.1016/0009-8981(92)90351-p

Cited by 30 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…For HbA1c, our data are in line with data of Torffvit et al [11] They have reported a correlation between urinary uromodulin and HbA1c for type 1 diabetic patients, but did not find a correlation with age, blood pressure, and antihypertensive treatment. [11] In this context, the difference between eAG, which directly correlates to HbA1c, and FPG has been previously demonstrated to be significantly different between subgroups in diabetic and nondiabetic subjects, and it has been suggested to be associated with glycemic control.…”

Section: Discussionsupporting

confidence: 82%

“…[11] In this context, the difference between eAG, which directly correlates to HbA1c, and FPG has been previously demonstrated to be significantly different between subgroups in diabetic and nondiabetic subjects, and it has been suggested to be associated with glycemic control. [35] In addition, the eAG/FPG ratio has been previously used to assess b-cell function.…”

Section: Discussionmentioning

confidence: 99%

“…Only few studies have also looked at this question assessing uromodulin in urine, but with contradictory results. [9][10][11][12] Moreover, there are at present, no data about the association between diabetes and uromodulin in blood.…”

Section: Introductionmentioning

confidence: 99%

“…[9,10] However other studies did not observe such an effect. [11,12] Whilst most studies in the past have looked at urinary uromodulin, two recent studies have measured uromodulin in blood. Both reported an association between uromodulin in plasma or serum and kidney function and recommended its further use as renal biomarker.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Serum uromodulin is associated with impaired glucose metabolism

et al. 2017

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Serum uromodulin is associated with impaired glucose metabolism

et al. 2017

View full text Add to dashboard Cite

“…TAL cells are the only cells in the body that form Tamm-Horsfall glycoprotein (THP), which is the major protein excreted in the urine [35]. The excretion rate is increased in diabetic patients with microalbuminuria and decreased in patients with macroalbuminuria [36]. In experimental diabetes of 80 days duration, the excretion rate is increased but the amount of THP in TAL cells and the amount of mRNA coding for THP is decreased [37].…”

Section: Discussionmentioning

confidence: 99%

Quantitative morphology of the rat kidney during diabetes mellitus and insulin treatment

Rasch

Dørup

1997

Diabetologia

View full text Add to dashboard Cite

Kidney enlargement in streptozotocin (STZ) diabetic rats is well documented [1][2][3][4]. This growth is mainly due to hyperplasia and to a lesser extent to cellular hypertrophy [2,5]. After insulin treatment and normalization of blood glucose concentration (BGS), kidney weight and glomerular volume decrease [6]. However, little is known about how fast the morphology changes following normalization of BG by insulin treatment and about the morphology of the diabetic kidney after insulin treatment or fasting.In the present study, quantitative estimation of each tissue component was performed during experimental diabetes and during insulin treatment. The results were compared with the kidney weight response and morphology in fasted diabetic animals and with the kidney weight and morphology in normal animals with hyperglycaemia induced by i. v. glucose injection.Information on kidney morphology in hyperglycaemia and normoglycaemia is a prerequisite to understanding better the physiological abnormalities found in diabetic patients. Diabetologia (1997) 40: 802-809 Quantitative morphology of the rat kidney during diabetes mellitus and insulin treatment Summary A morphometric study was performed on moderately hyperglycaemic streptozotocin diabetic rats after 10 and 50 days of diabetes, and on groups of rats that, after initial hyperglycaemia for 50 days, were insulin treated for 2 h or for 5, 15 or 38 days. A group of hyperglycaemic diabetic animals were fasted for 18 h. Another group of rats had acute hyperglycaemia induced by intravenous glucose injection. After 10 and 50 days of diabetes, kidney weight was increased by 55 and 93 %. Glomerular volume, tubule length, and tubular and interstitial volume increased in diabetic animals compared with controls. After 4 h insulin treatment, the kidney weight was 20 % decreased; after 5 days it was 31 % decreased. After 38 days the kidney weight was still 26 % greater than in controls. In diabetic animals, 18 h fasting induced a 30 % decrease in kidney weight. In normal animals, acute hyperglycaemia induced a 22 % increase in kidney weight. Volume fractions of most kidney structures remained similar in all groups. However, the glomerular volume fraction was smaller during kidney enlargement, and the tubular volume fraction was larger after induced hyperglycaemia compared with controls. In conclusion, high blood glucose levels in diabetic and normal animals are associated with increased kidney weight. In hyperglycaemic diabetic animals, normalization of blood glucose after insulin treatment or fasting was followed by a decrease in kidney weight. [Diabetologia (1997) 40: 802-809] Keywords Diabetes mellitus, experimental, kidney, diabetic nephropathy, morphometry.Received: 14 October 1996 and in revised form: 4 April 1997Corresponding author: Dr. R. Rasch, Department of Cell Biology, Institute of Anatomy, University of Aarhus, Building 234, DK-8000 Aarhus C, Denmark Abbreviations: CN, Controls; DH10, diabetic, hyperglycaemic for 10 days; DH50, diabetic, hyperglycaemic for 5...

show abstract

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Iijima¹,

Cho²,

Sakai³

et al. 2003

Clinical Laboratory Analysis

View full text Add to dashboard Cite

We have developed a new method for measuring total urinary protein using acid violet 6B (AV6B) pigment. Nine purified components of human urinary proteins and urine samples collected randomly from 123 diabetic outpatients were used. There were 62, 36, and 25 cases of prenephropathy, early nephropathy, and overt nephropathy, respectively. All samples were measured by Coomassie brilliant blue G 250 (CBB), pyrogallol red-molybdate (PRM), and AV6B methods using an optical photometer. In healthy subjects, the major components of urinary proteins, such as gamma-globulins, IgG, IgA Tamm-Horsfall protein, and transferrin, the reactivity values of the AV6B and PRM methods were similar. The CBB method was the least sensitive of the three methods. In the urine samples from diabetic patients, the urinary protein values measured by the AV6B method were higher than those measured by the CBB method in the prenephropathy stage. The values obtained by the AV6B method (y) correlated well with those from the CBB method (x) (y=1.243x+3.61, r=0.904). When the values from the AV6B method (y) were compared to those from the PRM method (x), correlation was low (y=1.406x-29.15, r=0.786). In conclusion, the AV6B method was more useful than the CBB and PRM methods for low levels of urinary protein.

show abstract

Tubular secretion of Tamm-Horsfall protein in type 1 (insulin-dependent) diabetes mellitus using a simplified enzyme linked immunoassay

Cited by 30 publications

References 26 publications

Serum uromodulin is associated with impaired glucose metabolism

Serum uromodulin is associated with impaired glucose metabolism

Quantitative morphology of the rat kidney during diabetes mellitus and insulin treatment

Development of a new method for measuring total urinary protein using acid violet 6B pigment

Contact Info

Product

Resources

About