Tubular polyvinyl alcohol composites used as vascular grafts: Manufacturing techniques and property evaluations

Lin, Jia Horng; Hu, Jin; Tu, Cheng Ying; Lee, Mong Chuan; Lu, Chao Tsang; Lu, Po; Chen, Yueh Sheng; Lou, Ching Wen

doi:10.1016/j.matlet.2016.12.097

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…The high failure rates of clinically utilized small diameter synthetic vascular grafts such as Dacron and ePTFE are likely due at least in part to the lack of endothelialization. Recent studies have demonstrated the applicability of PVA as a replacement of current commercial grafts for small diameter vascular grafts applications, including studies conducted in animal models. − ,− We had previously validated submillimeter PVA graft application for microvascular surgery in a rabbit animal model . Despite these promising results, the lack of endothelialization remains a bottleneck to further develop PVA as a viable choice for long-term success of small diameter vascular grafts.…”

Section: Discussionmentioning

confidence: 99%

One-Pot Covalent Grafting of Gelatin on Poly(Vinyl Alcohol) Hydrogel to Enhance Endothelialization and Hemocompatibility for Synthetic Vascular Graft Applications

Rizwan

Yao

Gorbet

et al. 2019

ACS Appl. Bio Mater.

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Cardiovascular diseases remain the leading cause of death worldwide. Patency rates of clinically utilized small diameter synthetic vascular grafts, such as Dacron and expanded polytetrafluoroethylene (ePTFE), to treat cardiovascular disease are inadequate because of the lack of endothelialization. Sodium trimetaphosphate (STMP) cross-linked poly(vinyl alcohol) (PVA) could be potentially employed as blood-compatible small diameter vascular graft for the treatment of cardiovascular disease. However, PVA severely lacks cell adhesion properties, and the efforts to endothelialize STMP-PVA have been insufficient to produce a functioning endothelium. To this end, we developed a one-pot method to conjugate cell-adhesive protein via hydroxyl-to-amine coupling using carbonyldiimidazole by targeting residual hydroxyl groups on cross-linked STMP-PVA hydrogel. Primary human umbilical vascular endothelial cells (HUVECs) demonstrated significantly improved cells adhesion, viability, and spreading on modified PVA. Cells formed a confluent endothelial monolayer, and expressed vinculin focal adhesions, cell–cell junction protein zonula occludens 1 (ZO1), and vascular endothelial cadherin (VE-Cadherin). Extensive characterization of the blood-compatibility was performed on modified PVA hydrogel by examining platelet activation, platelet microparticle formation, platelet CD61 and CD62P expression, and thrombin generation, which showed that the modified PVA was blood-compatible. Additionally, grafts were tested under whole, flowing blood without any anticoagulants in a nonhuman primate, arteriovenous shunt model. No differences were seen in platelet or fibrin accumulation between the modified-PVA, unmodified PVA, or clinical, ePTFE controls. This study presents a significant step in the modification of PVA for the development of next generation in situ endothelialized synthetic vascular grafts.

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Section: Discussionmentioning

confidence: 99%

One-Pot Covalent Grafting of Gelatin on Poly(Vinyl Alcohol) Hydrogel to Enhance Endothelialization and Hemocompatibility for Synthetic Vascular Graft Applications

Rizwan

Yao

Gorbet

et al. 2019

ACS Appl. Bio Mater.

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“…In their study on the manufacturing and performance of PVA knitted grafts, Jia-Horng Lin et al evaluated the circumferential mechanical properties of tubular weft knitted and warp knitted fabrics, demonstrating that both structures exhibited good compressive properties, with the warp knitted structure exhibiting a superior circumferential mechanical performance. They also optimized and modified the structure and function of the tubular fabrics [ 33 , 34 , 35 ]. Therefore, in the preparation, it was possible to select the appropriate warp and weft knitting structures according to the specific mechanical performance requirements.…”

Section: Knittingmentioning

confidence: 99%

The Influence of Textile Structure Characteristics on the Performance of Artificial Blood Vessels

et al. 2023

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Cardiovascular disease is a major threat to human health worldwide, and vascular transplantation surgery is a treatment method for this disease. Often, autologous blood vessels cannot meet the needs of surgery. However, allogeneic blood vessels have limited availability or may cause rejection reactions. Therefore, the development of biocompatible artificial blood vessels is needed to solve the problem of donor shortage. Tubular fabrics prepared by textile structures have flexible compliance, which cannot be matched by other structural blood vessels. Therefore, biomedical artificial blood vessels have been widely studied in recent decades up to the present. This article focuses on reviewing four textile methods used, at present, in the manufacture of artificial blood vessels: knitting, weaving, braiding, and electrospinning. The article mainly introduces the particular effects of different structural characteristics possessed by various textile methods on the production of artificial blood vessels, such as compliance, mechanical properties, and pore size. It was concluded that woven blood vessels possess superior mechanical properties and dimensional stability, while the knitted fabrication method facilitates excellent compliance, elasticity, and porosity of blood vessels. Additionally, the study prominently showcases the ease of rebound and compression of braided tubes, as well as the significant biological benefits of electrospinning. Moreover, moderate porosity and good mechanical strength can be achieved by changing the original structural parameters; increasing the floating warp, enlarging the braiding angle, and reducing the fiber fineness and diameter can achieve greater compliance. Furthermore, physical, chemical, or biological methods can be used to further improve the biocompatibility, antibacterial, anti-inflammatory, and endothelialization of blood vessels, thereby improving their functionality. The aim is to provide some guidance for the further development of artificial blood vessels.

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A Review: Textile Technologies for Single and Multi‐Layer Tubular Soft Tissue Engineering

Doersam

Tsigkou

Jones

2022

Adv Materials Technologies

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In addition, it requires a fundamental understanding of the structure and function of the target tissue type.Tubular tissues, such as vascular, respiratory, or intestinal systems supply the body with important nutrients and transport blood, air, food, or fluids. A defect in these tissues can considerably affect the patient's quality of life, for example, the required surgical reconstruction of connecting pathways between two anatomical structures can significantly reduce the patient's mobility. Current treatment strategies for diseased tubular tissues include transplantations of donor organs, autologous tissues, or implantable medical devices to restore tissue function. [2] However, transplants of donor organs or autologous tissue are limited due to availability, donor site morbidity, and risk of disease transmission. [3] Compared to natural body parts, implants have not yet reached the functionality, quality, or longevity (often need replacement after years). [3] Moreover, they must remain in the body for years, and material-specific compatibility problems can cause chronic inflammatory responses, thus limiting their clinical use. Being able to develop tissues outside the body provides a longterm alternative to organ transplantation that could offset the increasing discrepancy between the required number of donor organs needed and availability due to a growing and aging population and the higher life expectancy. [1] Additionally, eliminating the need for time-intensive therapy, for example, immune suppressants, and improving the patient quality of life. [1] Tubular tissues have many unifying structural and biomechanical characteristics despite their different functions. They consist of a multi-layered muscular wall structure of multiple different cell types. The different cell types are embedded in a surrounding environment, the extracellular matrix (ECM). [1] The ECM serves for the spatial organization of the cells and consists of fibrous structures, for example, collagen or elastin, which are proteins that form fiber bundles. [2] Fibrous structures can be found throughout the human body, whether in the architecture and ECM of specific tissue structures, such as arteries, lymphatic vessels, cartilage, or in the fibrous nature of nerves, muscles, ligaments, tendons. [4] A major aim of TE is to mimic the ECM and develop a 3D scaffold that will be seeded with native cells for tissue formation. Current scaffold designs of tubular tissues include foams, gel mattresses, sponges, meshes, and nanofibrous structures. [5] The scaffold serves as a template In cell-free scaffold tissue engineering (TE), an essential prerequisite is the scaffold design to promote cellular activities and tissue formation. The success is greatly dependent upon the nature of the scaffold including the composition, topography, and mechanical performance. Recent TE approaches use textile technologies to create biomimetic and functional scaffolds similar to the extracellular matrix (ECM). The hierarchical architecture of fiber to yarn to fabric a...

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Tubular polyvinyl alcohol composites used as vascular grafts: Manufacturing techniques and property evaluations

Cited by 7 publications

References 13 publications

One-Pot Covalent Grafting of Gelatin on Poly(Vinyl Alcohol) Hydrogel to Enhance Endothelialization and Hemocompatibility for Synthetic Vascular Graft Applications

One-Pot Covalent Grafting of Gelatin on Poly(Vinyl Alcohol) Hydrogel to Enhance Endothelialization and Hemocompatibility for Synthetic Vascular Graft Applications

The Influence of Textile Structure Characteristics on the Performance of Artificial Blood Vessels

A Review: Textile Technologies for Single and Multi‐Layer Tubular Soft Tissue Engineering

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