Tubgcp3 is a mitotic regulator of planarian epidermal differentiation

Ge, Cui; Lin, Cuikun; Zhang, Mengzhen; Yuan, Jingchen; Feng, Xiyan; Hao, Zhimin; Zhang, Shoutao; Tian, Qingnan

doi:10.1016/j.gene.2021.145440

Cited by 2 publications

(4 citation statements)

References 45 publications

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“…Immunostaining was carried out as previously reported. 32 , 33 The planarians 1–5 mm in length used for experience were killed with 5% NAC in PBS (phosphate buffered saline) for 5 min at room temperature, washed three times with PBST (phosphate buffered saline containing 0.1% TritonX‐100) at RT and fixed in PBST containing 4% paraformaldehyde for 2–4 h at 4°C. Then, worms were placed in 100% methanol for 1 h at −20°C and treated with 1% SDS in PBST for 10 min at RT.…”

Section: Methodsmentioning

confidence: 99%

“…Immunostaining was carried out as previously reported. 32,33 The planarians 1-5 mm in length used for experience were killed with 5% NAC in PBS (phosphate buffered saline) for 5 min at room temperature, washed three times with PBST (phosphate buffered saline containing 0.1% TritonX-100) at RT and fixed in PBST containing 4% paraformaldehyde for 2-4 h at 4 C. Then, worms were placed in 100% methanol for 1 h at À20 C and treated with 1% SDS in PBST for 10 min at RT. After rinsed in PBST, the samples were blocked with 10% goat serum in PBST for 2 h at 4 C or RT.…”

Section: Whole-mount Immunostainingmentioning

confidence: 99%

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MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

et al. 2023

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Adult stem cells (ASCs) are pluripotent cells with the capacity to self‐renew and constantly replace lost cells due to physiological turnover or injury. Understanding the molecular mechanisms of the precise coordination of stem cell proliferation and proper cell fate decision is important to regeneration and organismal homeostasis. The planarian epidermis provides a highly tractable model to study ASC complex dynamic due to the distinct spatiotemporal differentiation stages during lineage development. Here, we identified the myosin regulatory light chain (MRLC) homologue in the Dugesia japonica transcriptome. We found high expression levels of MRLC in wound region during regeneration and also expressed in late epidermal progenitors as an essential regulator of the lineage from neoblasts to mature epidermal cells. We investigated the function of MRLC using in situ hybridization, real‐time polymerase chain reaction and double fluorescent and uncovered the potential mechanism. Knockdown of MRLC leads to a remarkable increase in cell death, causes severe abnormalities during regeneration and homeostasis and eventually leads to animal death. The global decrease in epidermal cell in MRLC RNAi animals induces accelerated epidermal proliferation and differentiation. Additionally, we find that MRLC is co‐expressed with cdc42 and acts cooperatively to control the epidermal lineage development by affecting cell death. Our results uncover an important role of MRLC, as an inhibitor of apoptosis, involves in epidermal development.

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Section: Methodsmentioning

confidence: 99%

Section: Whole-mount Immunostainingmentioning

confidence: 99%

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

et al. 2023

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“…Knockdown of Tubgcp3 will result in lysis of amputated planarians. In the homeostatic Tubgcp3 (RNAi) planarians, darker region of epidermis will appear and lead to body lysis in the end 80 …”

Section: Mechanism Of Tissue‐specific Regenerationmentioning

confidence: 99%

“…In the homeostatic Tubgcp3(RNAi) planarians, darker region of epidermis will appear and lead to body lysis in the end. 80…”

Section: Epidermalmentioning

confidence: 99%

An insight into planarian regeneration

Han

Zhao

et al. 2022

Cell Proliferation

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Background: Planarian has attracted increasing attentions in the regeneration field for its usefulness as an important biological model organism attributing to its strong regeneration ability. Both the complexity of multiple regulatory networks and their coordinate functions contribute to the maintenance of normal cellular homeostasis and the process of regeneration in planarian. The polarity, size, location and number of regeneration tissues are regulated by diverse mechanisms. In this review we summarize the recent advances about the importance genetic and molecular mechanisms for regeneration control on various tissues in planarian. Methods: A comprehensive literature search of original articles published in recent years was performed in regards to the molecular mechanism of each cell types during the planarian regeneration, including neoblast, nerve system, eye spot, excretory system and epidermal. Results: Available molecular mechanisms gave us an overview of regeneration process in every tissue. The sense of injuries and initiation of regeneration is regulated by diverse genes like follistatin and ERK signaling. The Neoblasts differentiate into tissue progenitors under the regulation of genes such as egfr-3. The regeneration polarity is controlled by Wnt pathway, BMP pathway and bioelectric signals. The neoblast within the blastema differentiate into desired cell types and regenerate the missing tissues. Those tissue specific genes regulate the tissue progenitor cells to differentiate into desired cell types to complete the regeneration process. Conclusion: All tissue types in planarian participate in the regeneration process regulated by distinct molecular factors and cellular signaling pathways. The neoblasts play vital roles in tissue regeneration and morphology maintenance. These studies provide new insights into the molecular mechanisms for regulating planarian regeneration.

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Tubgcp3 is a mitotic regulator of planarian epidermal differentiation

Cited by 2 publications

References 45 publications

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

MRLC controls apoptotic cell death and functions to regulate epidermal development during planarian regeneration and homeostasis

An insight into planarian regeneration

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