“…Endogenous peroxidases were blocked by incubation in 10% methanol-1% H 2 O 2 solution (15min) followed by 3% normal horse serum solution. Then the sections were incubated at 4ºC overnight with one of the primary antibodies against 4Rtau, 3Rtau, amino acids [14][15][16][17][18][19][20][21][22][23][24][25][26] (antibody 499), amino acids 229-233 (antibody 229), amino acids 394-398), specific phospho-tau Thr181, Ser199, Thr231, Ser262 and Ser422, double-phosphorylation sites Ser202-Thr205 (clone AT8), Ser396-404 (PHF1) and Thr212-Ser214 (tau-100), conformational tau modifications at amino acids 5-15 (Alz50) and amino acids 312-322 (MC-1), and tau truncated at aspartic acid 421 (tau-C3). Other sections were incubated with one of the following antibodies against glial fibrillary acidic protein (GFAP): P-GFAP Ser8, phosphorylated tuberin (P-tuberin Ser939), superoxide dismutase 2 (SOD2), aquaporine 4 (AQP4), phosphorylated p38 (p38-P Thr180-Tyr182), phosphorylated protein kinase A α/β (PKA-P α/β Thr197), glutamate transporter solute carrier family 1, member 2 (GLT-1/EAAT2), vimentin and YKL-40.…”