Tuberculous anergic sera or purified protein derivative treatment induces modification in lymphocyte transformation of cells from patients with tuberculosis

Mejia-Lopez, Herlinda; Vaca-García, O; Cristerna-Aguirre, J M; Cruz-Chávez, A; Selman-Lama, M; Taylor, Mária Lucía

doi:10.1128/jcm.28.2.344-349.1990

Cited by 3 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analysis of T‐lymphocyte cytokine response is important in studying the immune response to mycobacterial tuberculosis and clinical course of the disease. In agreement with previously published studies, our results showed that the tuberculin‐negative patients with active pulmonary tuberculosis were mainly those with more serious forms of the disease in whom a prolonged duration of the disease should be anticipated [14, 26, 27]. A growing body of evidence suggest that the human immune response to infection is similarly mediated by T‐cell subpopulations secreting specific cytokine patterns.…”

Section: Discussionsupporting

confidence: 90%

Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis

et al. 2007

View full text Add to dashboard Cite

The possible immunological relationship between the pattern of Th1/Th2 cytokine production and tuberculin reactivity was assessed in patients with active Mycobacterium tuberculosis infection. The production of the intracellular cytokines interferon (IFN)‐γ and interleukin‐4 (IL‐4) was measured in CD4+ and CD8+ T cells obtained from peripheral blood and bronchoalveolar lavage fluid (BALF) of 20 tuberculin skin‐positive patients and compared with the findings recorded in nine tuberculin skin‐negative patients with active pulmonary tuberculosis. Upon stimulation with phorbol 12‐myristate acetate/ionomycin for 6 h, tuberculin‐negative patients had a significantly higher proportion of IFN‐γ‐producing CD4+ T lymphocytes in BALF than in peripheral blood, while both CD4+ and CD8+ T‐lymphocyte subsets in BALF of tuberculin‐positive patients secreted more IFN‐γ than their peripheral blood counterparts. Tuberculin‐negative patients had a significantly higher proportion of IFN‐γ‐producing CD4+ T lymphocytes in peripheral blood than tuberculin‐positive patients. There was no significant difference in the production of IFN‐γ by BALF CD4+ T lymphocytes, or by either peripheral blood or BALF CD8+ T lymphocytes. In two tuberculin‐negative patients, peripheral blood CD4+ T lymphocytes produced IL‐4. Study results suggested a higher immune activity in the blood of tuberculin‐negative patients, with an increased lymphocyte activity in BALF versus peripheral blood in both patient groups.

show abstract

Section: Discussionsupporting

confidence: 90%

Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis

et al. 2007

View full text Add to dashboard Cite

show abstract

Characterization of an Inhibitory Seric Factor From Tuberculosis Anergic Patients That Acts on non-Adherent PPD Reactive Cells

Taylor

Elizondo²,

Mejia-Lopez³

et al. 1995

Immunological Investigations

View full text Add to dashboard Cite

Non-adherent cells from PPD+ tuberculosis patients (TBP PPD+) and from healthy individuals treated with whole tuberculosis anergic immune sera or with its protein A-Sepharose IgG fraction, or with sera fraction separated by PPD-Sepharose chromatography, were submitted to immunofluorescence assays. Anti-human IgG or IgM FITC-conjugate were used to reveal the assays, and results were expressed by a fluorescence percentage or fluorescence index. The presence of IgG over the surface of PPD+ non-adherent cells was detected. High fluorescence percentages were observed only in those PPD+ cells treated with whole anergic serum or with its IgG fraction. Positive fluorescence index values were obtained only in those PPD+ cells treated with anergic serum, meanwhile fluorescence index was always negative when non-bound fractions from PPD-Sepharose were used. Results suggest that non-adherent population are the cell targets for the serum inhibitory factor, which previously has been detected to inhibit antigen response in PPD reactive cells and, point out the specific behavior of this factor, since it was eliminate by PPD-Sepharose chromatography. The IgG nature of the factor was demonstrated by SDS-PAGE and immunoelectrophoresis.

show abstract

BCG Specificity of an inhibitory seric factor from tuberculosis anergic patients that acts on non-adherent ppd reactive cells

Mejia-Lopez

Martínez-García

Zenteno

et al. 1997

Immunological Investigations

View full text Add to dashboard Cite

The effect of tuberculosis anergic immune sera adsorbed with BCG was studied on cocultures of adherent and non-adherent cells from PPD+ tuberculosis patient (TBP PPD+). This effect on the cocultures was quantified by lymphocyte transformation (LT) test using PPD as antigen. Only those cocultures with non-adherent cells from TBP PPD+ patients treated with anergic sera, inhibited the LT response induced by PPD, whereas sera adsorption with BCG eliminated the inhibitory effect.

show abstract

Tuberculous anergic sera or purified protein derivative treatment induces modification in lymphocyte transformation of cells from patients with tuberculosis

Cited by 3 publications

References 10 publications

Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis

Cytokine Profile of T lymphocytes from Peripheral Blood and Bronchoalveolar Lavage Fluid in Patients with Active Pulmonary Tuberculosis

Characterization of an Inhibitory Seric Factor From Tuberculosis Anergic Patients That Acts on non-Adherent PPD Reactive Cells

BCG Specificity of an inhibitory seric factor from tuberculosis anergic patients that acts on non-adherent ppd reactive cells

Contact Info

Product

Resources

About