Tuberculosis – burden and serodiagnosis

Mohiuddin,

doi:10.3329/imcjms.v14i1.47457

Cited by 2 publications

(1 citation statement)

References 37 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The advantages of such diagnostics in comparison with the detection and identification of the pathogen itself consist in the relatively simple sampling, the reproducible results that do not depend on the choice of the sampling site or that slowly change for the patient over time, and the excluded need to grow the pathogen until it reaches the detectable concentration. Serodiagnostics is actively used for many widespread and socially significant infections, including tuberculosis, human immunodeficiency virus, and more [ 4 , 5 , 6 , 7 , 8 ]. Particular interest in this diagnostic method has arisen this year due to the coronavirus pandemic [ 9 , 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Lateral Flow Serodiagnosis in the Double-Antigen Sandwich Format: Theoretical Consideration and Confirmation of Advantages

Sotnikov

Zherdev

Dzantiev

2020

Sensors

View full text Add to dashboard Cite

Determination of the presence in the blood of antibodies specific to the causative agent of a particular disease (serodiagnosis) is an effective approach in medical analytical chemistry. Serodiagnostics performed in the lateral flow immunoassay format (immunochromatography) meet the modern requirements for point-of-care testing and are supported by existing technologies of large-scale diagnostic tests production, thus increasing the amount of attention in a tense epidemiological situation. For traditional lateral flow serodiagnostics formats, a large number of nonspecific immunoglobulins in the sample significantly reduces the degree of detectable binding. To overcome these limitations, an assay based on the formation of immobilized antigen-specific antibody-labeled antigen complexes detection was proposed. However, the requirements for its implementation, providing maximum sensitivity, have not been established. This article describes the mathematical model for the above assay. The influence of the ratio of reagent concentrations on the analysis results is considered. It is noted that the formation of specific antibody complexes with several labeled antigens is the main limiting factor in reducing the detection limit, and methods are proposed to minimize this factor. Recommendations for the choice of the assay conditions, following from the analysis of the model, are confirmed experimentally.

show abstract