Tuber metabolic profiling of resistant and susceptible potato varieties challenged with Phytophthora infestans

Hamzehzarghani, Habiballah; Vikram, A.; Abu-Nada, Y.; Kushalappa, Ajjamada C.

doi:10.1007/s10658-015-0840-3

Cited by 10 publications

(9 citation statements)

References 38 publications

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“…These oxylipins act as antimicrobial compounds and also induce the expression of pathogenesis-related proteins (Bollina et al 2011). Significantly enhanced accumulation of trihydroxybutyrate was also reported in infected leaves of LLS resistant potato cultivar (Hamzehzarghani et al 2016) and inoculated leaves of Alternaria leaf spot resistant genotypes of groundnut (Mahatma et al 2019). Thus induction of 2,3,4trihydroxybutyric acid in resistant genotypes confirms its role as a defense metabolite.…”

Section: Non-targeted Metabolomics Using Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 64%

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

Mahatma

Thawait

Jadon

et al. 2021

Physiol Mol Biol Plants

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Late leaf spot (LLS) caused by fungi Passalora personata is generally more destructive and difficult to control than early leaf spot. The aim of this study was to decipher biochemical defense mechanism in groundnut genotypes against P. personata by identifying resistance specific biomarkers and metabolic pathways induced during host-pathogen interaction. Metabolomics of non-infected and infected leaves of moderately resistant (GPBD4 and ICGV86590), resistant (KDG128 and RHRG06083) and susceptible (GG20, JL24 and TMV2) genotypes was carried out at 5 days after infection (65 days after sowing). Nontargeted metabolite analysis using GC-MS revealed total 77 metabolites including carbohydrates, sugar alcohols, amino acids, fatty acids, polyamines, phenolics, terpenes and sterols. Variable importance in projection (VIP) measure of partial least squares-discriminant analysis (PLS-DA) showed that resistant and moderately resistant genotypes possessed higher intensities of ribonic acid, cinnamic acid, malic acid, squalene, xylulose, galactose, fructose, glucose, b-amyrin and hydroquinone while susceptible genotypes had higher amount of gluconic acid 2-methoxime, ribo-hexose-3-ulose and gluconic acid. Heat map analysis showed that resistant genotypes had higher intensities of b-amyrin, hydroquinone in non-infected and malic acid, squalene, putrescine and 2,3,4-trihydroxybutyric acid in infected leaves. Dendrogram analysis further separated resistant genotypes in the same cluster along with infected moderately resistant genotypes. The most significant pathways identified are: linoleic acid metabolism, flavone and flavonol biosynthesis, cutin, suberin and wax biosynthesis, pentose and glucuronate interconversions, starch and sucrose metabolism, stilbenoid biosynthesis and ascorbate and aldarate metabolism. Targeted metabolite analysis further confirmed that resistant genotypes possessed higher content of primary metabolites sucrose, glucose, fructose, malic acid and citric acid. Moreover, resistant genotypes possessed higher content of salicylic, coumaric, ferulic, cinnamic, gallic acid (phenolic acids) and kaempferol, quercetin and catechin (flavonols). Thus metabolites having higher accumulation in resistant genotypes can be used as biomarkers for screening of LSS resistant germplasm. These results unravel that higher amount of primary metabolites leads to stimulate the accumulation of more amounts of secondary metabolites such as phenolic acid, flavanols, stilbenes and terpenoids (squalene and b-amyrin) biosynthesis which are ultimately involved in defense mechanism against LLS pathogen.

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Section: Non-targeted Metabolomics Using Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 64%

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

Mahatma

Thawait

Jadon

et al. 2021

Physiol Mol Biol Plants

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“…Scopoletin has been shown to correlate with plant resistance against several pathogen in tobacco, and to possess antifungal activity ( El Oirdi et al, 2010 ; Sun et al, 2014 ; Yang et al, 2018 ). Furthermore, several hydroxycoumarins have been associated with resistance to late blight in potato leaves and tubers ( Yogendra et al, 2014 ; Yogendra et al, 2015 ; Hamzehzarghani et al, 2016 ). In our antifungal bioassay, scopoletin, and especially esculetin (which showed both high RRC and RRI scores), exhibited antifungal activity against C. coccodes at 500 µM ( Figure 8 ).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, flavonoid glycosides, such as rutin and nicotiflorin, are important for determining resistance against several plant pathogens in a number of plants, including potatoes ( Bollina et al, 2010 ; El Hadrami et al, 2011 ; Henriquez et al, 2012 ; Kröner et al, 2012 ; Pushpa et al, 2014 ). Resistance-related metabolites to late blight have also been identified in potato tubers, and include glucosinolate derivatives, coumarins, and organic acids ( Hamzehzarghani et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)

et al. 2020

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“…In einer Vielzahl von Studien wurden die molekularen Abwehrmechanismen der Kartoffelpflanze in Folge einer P. infestans-Infektion untersucht (Andreu et al 2001, Göbel et al 2002, Matthews et al 2005, Yogendra et al 2014, Hamzehzarghani et al 2016, Tomita et al 2017. Eine Stoffgruppe, die im Zusammenhang mit dem Schutz vor P. infestans gebracht wird, sind die Phytoalexine.…”

Section: Kraut Und Knollenfäuleunclassified

“…In weiteren Metabolomstudien wurde eine Hochregulierung verschiedener Metaboliten, wie Zimtsäure, 3-Indolacetonitril oder Malonsäure, postuliert. Jedoch fehlen in all diesen Studien quantitative Daten sowie Daten zur Hemmeigenschaft der jeweiligen Metaboliten gegenüber P. infestans (Yogendra et al 2015, Hamzehzarghani et al 2016, Tomita et al 2017. Die molekulare Schutzreaktion der Pflanze als Reaktion auf die Infektion ist äußerst komplex und bis heute noch nicht vollständig verstanden.…”

Section: In Einer Ungerichtetenunclassified

Molekulare Charakterisierung des Einflusses biotischer und abiotischer Stressfaktoren auf das Metabolom von Kartoffeln (Solanum tuberosum L.)

et al. 2021

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Tuber metabolic profiling of resistant and susceptible potato varieties challenged with Phytophthora infestans

Cited by 10 publications

References 38 publications

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

Metabolic profiling for dissection of late leaf spot disease resistance mechanism in groundnut

Insights on the Structural and Metabolic Resistance of Potato (Solanum tuberosum) Cultivars to Tuber Black Dot (Colletotrichum coccodes)

Molekulare Charakterisierung des Einflusses biotischer und abiotischer Stressfaktoren auf das Metabolom von Kartoffeln (Solanum tuberosum L.)

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