Tuber development phenotypes in adapted and acclimated, drought-stressed Solanum tuberosum ssp. andigena have distinct expression profiles of genes associated with carbon metabolism

Watkinson, Jonathan I.; Hendricks, Lori; Sioson, Allan A.; Heath, Lenwood S.; Bohnert, Hans J.; Grene, Ruth

doi:10.1016/j.plaphy.2007.10.020

Cited by 49 publications

(26 citation statements)

References 34 publications

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“…Some recent studies have also addressed the question of identifying adaptive genes by analyzing transcriptional changes upon water stress in drought-adapted Solanum tuberosum ssp. andigena (Schafleitner et al 2007;Vasquez-Robinet et al 2008;Watkinson et al 2008 Fig. 2 Validation of microarray analysis by semiquantitative RT-PCR.…”

Section: Discussionmentioning

confidence: 99%

“…Adaptive genes are potentially more appealing for containing yield penalty under water stress and for successful development of drought-tolerant varieties (Blum 2005). This question was recently addressed for potato crop by comparing the molecular response to long-term water limiting conditions of adapted Andean potato genotypes, leading to the identification of some potential candidate adaptive genes (Schafleitner et al 2007;Vasquez-Robinet et al 2008;Watkinson et al 2008). Ideally, comparative transcription profiling of the response to shock or gradual water stress conditions might also provide complementary information, since it is presumable that shock conditions induce likely genes in the sensing, signalling and immediate response, while genes regulated by a gradual water stress imposition are more related to a long-term adaptive response.…”

Section: Introductionmentioning

confidence: 99%

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Differential gene regulation in potato cells and plants upon abrupt or gradual exposure to water stress

et al. 2010

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Potato cDNA microarray slides (TIGR 1 K) were used to compare changes in gene expression of two potato cell populations subjected to shock or gradual exposure to PEG-mediated water stress. A total of 180 transcripts were found to be statistically up- or down-regulated in PEG-shocked or gradually stressed cells, compared to untreated control cells. Up-regulated genes in shocked cells were prevalently involved in carbohydrate metabolism, cellular communication, and signal transduction. In cells stressed gradually, the most represented induced genes were those involved in signal transduction, in response to environmental signals and in the regulation of transcription and translation machineries, including the heat shock protein 90-2, ribosomal and RNA-binding proteins. The expression of selected up-regulated genes was also tested in potato plants subjected to slow soil-drying conditions. Interestingly, the rgga gene, encoding an RNA-binding protein, was strongly induced in both leaves and roots of water-stressed potato plants, supporting its putative function in adaptive mechanisms to water stress. Pointing to differences in gene expression between shock-like and adaptive response, our findings might contribute to the controversial debate on the biological function of stress-responsive genes in cell damage repair or in restoring homeostasis and elaborating longer term responses for adaptation

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential gene regulation in potato cells and plants upon abrupt or gradual exposure to water stress

et al. 2010

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“…Antioxidant defense of metallothioneins is activated at both adaptation and acclimation stages while genes involved in the flavonoid pathway are induced in drought adaptation since they show an induced basal level of expression in droughtadapted accession. Since the tuber development differed between three accessions of potato exposed to drought stress, a follow-up transcriptomic study was carried out to correlate the expression of genes associated with carbon metabolism with the tuber development phenotypes under drought (Watkinson et al, 2008). Adapted accession VTSA01 showed starch deficiency while intermediate accession VTSA02 showed normal starch deposition due to the alterations of expression of genes involved in sucrose and starch metabolism, such as sucrose synthase, starch phosphorylase, and glycogen bound starch synthase, as well as genes associated with trehalose and respiration metabolisms.…”

Section: Potato Transcriptomics In Drought and Heat Stressesmentioning

confidence: 99%

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

Aksoy

Demirel²,

Öztürk³

et al. 2015

Turk J Bot

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Sustainable potato production practices are crucial for food security and social sustainability in the future since potato is a highly nutritious food and it is considered as one of the most promising crops to reduce human hunger and poverty in the world due to its high yield potential. However, being a temperate crop, potato is exposed to various environmental stresses, including extended periods of drought and heat. The majority of potato genomics, transcriptomics, and transgenics studies concentrate on the characterization of molecular mechanisms governing cold hardiness of tubers and response and tolerance mechanisms against diseases. Likewise, potato breeding studies focus on increasing the yield, extending the postharvest storage, and developing cultivars that withstand biotic stresses. The number of genomics, transcriptomics, and transgenics studies of drought and heat tolerance in potato is limited, although they are necessary state-of-the-art research procedures to characterize and identify the regulatory mechanism underlying any stresses in order to develop new crop varieties that can tolerate harsh environmental conditions. For these reasons, this review focuses on recent advances in genomics, transcriptomics, and transgenics of drought and heat tolerance in potato.

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“…These proteins that are concentrated in the chloroplast and the cytosol protect photosynthetic apparatus by non-enzymatic ROS-scavenging process (Lemaire et al 2007). These thioredoxin genes are known to be light-dependent regulators of carbon metabolizing enzymes including that of Calvin cycle, especially starch biosynthesis and maintenance of sink strength under waterlimiting conditions (Watkinson et al 2008).…”

Section: Functional Annotation Of Water Deficit Stress Upregulated Estsmentioning

confidence: 99%

Identification of Water Deficit Stress Upregulated Genes in Sugarcane

et al. 2010

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Sugarcane represents an important renewable source among biofuel crops with high capability to assimilate carbon among the C4 plants. Limited availability of freshwater renders this crop uneconomical, warranting the necessity for the development of varieties with higher water use efficiency and tolerant to water deficiency stress. Sugarcane variety cv. Co740 was subjected to varied levels of water deficiency stress to isolate transcripts differentially expressed to the imposed stress. The leaf relative water content was used as a measure to estimate the stress response. PCR-based cDNA suppression subtractive hybridization technique was applied to construct forward subtracted library for differentially expressed genes under stress. Dot blot-selected 158 clones showing elevated response were sequenced, of which 62% resembled similarity with known functional genes, 12% with hypo-

show abstract

Tuber development phenotypes in adapted and acclimated, drought-stressed Solanum tuberosum ssp. andigena have distinct expression profiles of genes associated with carbon metabolism

Cited by 49 publications

References 34 publications

Differential gene regulation in potato cells and plants upon abrupt or gradual exposure to water stress

Differential gene regulation in potato cells and plants upon abrupt or gradual exposure to water stress

Recent advances in potato genomics, transcriptomics, and transgenicsunder drought and heat stresses: a review

Identification of Water Deficit Stress Upregulated Genes in Sugarcane

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