Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column

Yamada, Kento; Kan, Hyo; Tsukagoshi, Kazuhiko

doi:10.1016/j.talanta.2018.02.046

Cited by 12 publications

(11 citation statements)

References 17 publications

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“…The theoretical elution times of the TRDC system were determined assuming that the stationary phase is not static and flows slowly relative to the mobile phase, according to a parabolic curve of linear velocity under laminar flow conditions. The parabolic curves for the organic solvent-rich and water-rich eluent solution can be drawn [10,12] under the present conditions as shown in Fig. 6.…”

Section: Estimation Of Elution Times For Analytesmentioning

confidence: 90%

“…Under certain conditions, annular flow consisting of inner and outer phases can be observed as a phase-separation multiphase flow, which we call tube radial distribution flow (TRDF). Novel open-tubular capillary chromatography has been performed based on specific TRDF, which we name separation method tube radial distribution chromatography (TRDC) [9,10]. The outer phase in TRDF acts as a pseudo-stationary phase under laminar flow conditions during chromatographic separation.…”

Section: Introductionmentioning

confidence: 99%

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Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

et al. 2019

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A method of tube radial distribution chromatography (TRDC) based on an annual flow with inner and outer phases created through phase transformation in an open-tubular capillary was developed. The outer phase works as a pseudo-stationary phase under laminar flow conditions in TRDC. Two model fluorescent analytes, hydrophobic perylene and hydrophilic Eosin Y, were separated by TRDC using a water/acetonitrile/ethyl acetate mixed solution (3:8:4 volume ratio, organic solvent-rich or 4:3:1 volume ratio, water-rich) as an eluent solution and a fused-silica capillary tube (75 m inner diameter and 120 cm length/100 cm effective length) as a separation column. We observed for the first time microfluidic behavior of fluorescent analytes that distributed and separated in the capillary tube visually by fluorescence microscope-charge coupled device camera. The separation mechanism in the TRDC was confirmed with visual fluorescence data and the obtained chromatographic data with UV detection. Furthermore, the elution times of perylene and Eosin Y were calculated based on their retention factors for the two phases and laminar flow conditions. The data and results obtained support the proposed separation mechanism in TRDC.

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Section: Estimation Of Elution Times For Analytesmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

et al. 2019

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“…In contrast, when the mixed solution was fed into a microspace, such as capillary tubes and microchannels on a microchip, annular flow with inner and outer phases was generated under certain conditions; we call this "Tube Radial Distribution Flow" (TRDF). The specific microfluidic flow, TRDF, was applied to capillary liquid chromatography [8] and micro-extraction [9].…”

Section: Introductionmentioning

confidence: 99%

Development of <i>α</i>-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution

Kan¹,

Mizutani²,

Tsukagoshi³

2020

AJAC

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Peroxyoxalate chemiluminescence was, for the first time, examined by using ternary mixed solutions of water-hydrophilic/hydrophobic organic solvent. Eosin Y as a model fluorescence compound was dissolved with the ternary solutions of water (1.0 mM carbonate buffer, pH 9.0)-acetonitrile-ethyl acetate, water-rich of 15:3:2 volume ratio and organic solvent-rich of 3:8:4 volume ratio, to which bis(2,4,6-trichlorophenyl) oxalate and hydrogen peroxide chemiluminescence reagent were added. The chemiluminescence observed with the ternary solutions, especially the organic solvent-rich solution, showed a larger signal than that observed with the water only solution or water-acetonitrile mixed solution. Chemiluminescence in the presence of twenty types of α-amino acid was similarly examined by using the ternary organic solvent-rich solution. The chemiluminescence of three α-amino acids with fluorescence properties was enhanced with the ternary solution. The data reported here may contribute to development of a new, sensitive peroxyoxalate chemiluminescence detection system.

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“…TRDF, annular flow in a micro-space, has been applied to new types of chromatography, extraction, mixing, and micro-reactor systems. [4][5][6] We previously attempted to separate and collect the two phases, i.e., the inner and outer phases, in the TRDF by using a micro-flow system with different inner diameter double tubes 7,8 and a microchip incorporating a triple-branched microchannel. 9,10 The flow lines were complicated, and it was difficult to use them in practice.…”

Section: Introductionmentioning

confidence: 99%

Phase Separation and Collection of Annular Flow by Phase Transformation

et al. 2019

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A polyethylene glycol/citrate mixed solution was fed into a single channel of a Y-type micro-channel on a microchip as an aqueous two-phase system. A phase separation multi-phase flow with a liquid-liquid interface was generated due to a phase transformation. An annular flow, one of the flow types in the phase separation multi-phase flow, was observed through bright-field microscopy. The flow consisted of citrate-rich inner and polyethylene glycol-rich outer phases. We attempted to separate and collect the two phases in the single channel into two separate Y-type channels. When the pressure losses for the separated channels were not very different, we observed symmetric flow in the Y-type channel. When the pressure losses were quite different, the polyethylene glycol-rich phase with higher viscosity was selectively distributed to the separated channel with lower pressure loss. Thus, the polyethylene glycol-rich phase was successfully and intentionally collected from the chosen Y-type channel via the creation of annular flow in the single channel.

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Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column

Cited by 12 publications

References 17 publications

Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

Development of <i>α</i>-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution

Phase Separation and Collection of Annular Flow by Phase Transformation

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Tube radial distribution chromatography system developed by combining commercially available HPLC system and open-tubular capillary tube as separation column

Cited by 12 publications

References 17 publications

Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

Confirmation of Separation Mechanism Through Visualization of Microfluidic Behavior of Fluorescent Analytes in Tube Radial Distribution Chromatography

Development of &lt;i&gt;α&lt;/i&gt;-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution

Phase Separation and Collection of Annular Flow by Phase Transformation

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Development of <i>α</i>-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution