Tualang honey inhibits cell proliferation and promotes apoptosis of human lung adenocarcinoma cells via apoptosis signaling pathway

Amran, Nazirah; Wan-Ibrahim, Wan Izlina; Rashid, Nurshamimi Nor; Ali, Johari Mohd; Abdul‐Rahman, Puteri Shafinaz

doi:10.1016/j.eujim.2020.101149

Cited by 5 publications

(4 citation statements)

References 36 publications

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“…These findings suggest that QV0 inhibits the mitochondrial OCR in mesothelioma cells, causing mitochondrial dysfunction-induced apoptosis. Our findings are in agreement with those of a study by Amran et al, which demonstrated that Tualang honey inhibits cell proliferation and induces cell apoptosis with reduced mitochondrial membrane potential in the human breast cancer cell lines, MCF-7 and MDA-MB-231 25 . Moreover, we found that the anti-cancer effects of QV0 on proliferation, apoptosis and mitochondrial function were cancer cell-specific, with QV0 having no effect on the non-malignant cells, thus suggesting its potential to be used as a novel anti-cancer drug that induces minimal damage or alteration to healthy non-malignant cells.…”

Section: Discussionsupporting

confidence: 94%

Leptospermum extract (QV0) suppresses pleural mesothelioma tumour growthin vitroandin vivoby mitochondrial dysfunction associated apoptosis

Shi¹,

Zhang

Yu³

et al. 2022

Preprint

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Pleural mesothelioma (PM) is a highly aggressive, fast-growing asbestos-induced cancer with limited effective treatments. There has been an interest in using naturally occurring anticancer agents derived from plant materials for the treatment of PM. However, it is unclear if aqueous extract from the Leptospermum polygalifolium (QV0) has activity against PM. Here we investigated the anti-cancer property of QV0 in vitro and in vivo. Animals treated with Defender (QV0 dietary supply) exhibited a reduced tumour size over 30 days, which was associated with an average extended of seven days mouse life. There was no liver toxicity, nor increased blood glucose post-treatment in animals treated with Defender. Moreover, QV0 suppressed the growth of 13 cancer cell lines in a dose-dependent manner, effective at concentrations as low as 0.02% w/v. This response was found to be associated with inhibited cell migration, proliferation, and colony formation, but without evident cell cycle alteration. We observed mitochondrial dysfunction post QV0 treatment, as evidenced by significantly decreased basal and maximal oxygen consumption rates. Significantly enhanced tumour apoptosis was observed in the Defender-treated animals, correlating with mitochondrial dysfunction. To the best of our knowledge, this study constitutes the first demonstration of an improved host survival (without adverse effects) response in a QV0-treated PM mouse model, associated with an evident inhibition of PM cell growth and mitochondrial dysfunction-related enhancement of tumour apoptosis.

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Section: Discussionsupporting

confidence: 94%

Leptospermum extract (QV0) suppresses pleural mesothelioma tumour growthin vitroandin vivoby mitochondrial dysfunction associated apoptosis

Shi¹,

Zhang

Yu³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…These findings suggest that QV0 inhibits the mitochondrial OCR in mesothelioma cells, causing mitochondrial dysfunction-induced apoptosis. Our findings are in agreement with those of a study by Amran et al., which demonstrated that Tualang honey inhibits cell proliferation and induces cell apoptosis with reduced mitochondrial membrane potential in the human breast cancer cell lines MCF-7 and MDA-MB-231 ( 33 ). Moreover, we found that the anti-cancer effects of QV0 on proliferation, apoptosis, and mitochondrial function were cancer cell-specific, with QV0 having no effect on non-malignant cells ( Figure 1 ), thus suggesting its potential to be used as a novel anti-cancer drug that induces minimal damage or alteration to healthy non-malignant cells.…”

Section: Discussionsupporting

confidence: 93%

Leptospermum extract (QV0) suppresses pleural mesothelioma tumor growth in vitro and in vivo by mitochondrial dysfunction associated apoptosis

Shi

Zhang

et al. 2023

Front. Oncol.

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Pleural mesothelioma (PM) is a highly aggressive, fast-growing asbestos-induced cancer with limited effective treatments. There has been interest in using naturally occurring anticancer agents derived from plant materials for the treatment of PM. However, it is unclear if an aqueous extract from Leptospermum polygalifolium (QV0) has activity against PM. Here we investigated the anti-cancer properties of QV0 and Defender® (QV0 dietary formula) in vitro and in vivo, respectively. QV0 suppressed the growth of eight PM cell lines in a dose-dependent manner, effective at concentrations as low as 0.02% w/v (equivalent to 0.2 mg/ml). This response was found to be associated with inhibited cell migration, proliferation, and colony formation but without evident cell cycle alteration. We observed mitochondrial dysfunction post-QV0 treatment, as evidenced by significantly decreased basal and maximal oxygen consumption rates. Ten SCID mice were treated with 0.25 mg/g Defender® daily and exhibited reduced tumor size over 30 days, which was associated with an average extension of seven days of mouse life. There was no evidence of liver toxicity or increased blood glucose post-treatment in animals treated with Defender®. Significantly enhanced tumor apoptosis was observed in the Defender®-treated animals, correlating to mitochondrial dysfunction. Lastly, the high levels of polyphenols and antioxidant properties of QV0 and Defender® were detected in HPLC analysis. To the best of our knowledge, this study constitutes the first demonstration of an improved host survival (without adverse effects) response in a QV0-treated PM mouse model, associated with evident inhibition of PM cell growth and mitochondrial dysfunction-related enhancement of tumor apoptosis.

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“…99 The pro-apoptosis and antiproliferative effects of Tualang honey on adenocarcinoma cells were proved by MTT and annexin V-FITC assay. 100 Kelulut honey had a promising cytotoxic effect on lung adenocarcinoma epithelial cell line and was capable of inducing cell cycle arrest at G2/M phase. 101 Moroccan honey showed significant cytostatic effects on HCT cells, and a strong positive correlation was found between the cytostatic activity and the content of antioxidant substances (phenols, flavonoids and flavonols).…”

Section: Anticancer Activitymentioning

confidence: 99%

“…The mechanisms of anticancer might be attributed to the apoptotic, antiproliferative, antitumor necrosis, antioxidant, anti‐inflammatory, estrogenic and immunomodulatory activities of honey 99 . The pro‐apoptosis and antiproliferative effects of Tualang honey on adenocarcinoma cells were proved by MTT and annexin V‐FITC assay 100 . Kelulut honey had a promising cytotoxic effect on lung adenocarcinoma epithelial cell line and was capable of inducing cell cycle arrest at G2/M phase 101 .…”

Section: Functional Characteristicsmentioning

confidence: 99%

Composition, functional properties and safety of honey: a review

Wang

Lin

et al. 2023

J Sci Food Agric

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Honey has been used not only as a food source but also for medicinal purposes. Recent studies have indicated that honey exhibits antioxidant, hepatoprotective, hypolipidemic, hypoglycemic and anti‐obesity properties, as well as anticancer, anti‐atherosclerotic, hypotensive, neuroprotective and immunomodulatory activities. These health benefits of honey could be attributed to its wide range of nutritional components, including polysaccharides and polyphenols, which have been proven to possess various beneficial properties. It is notable that the composition of honey can also be affected by nectar, season, geography and storage condition. Moreover, the safety of honey requires caution to avoid any potential safety incidents. Therefore, this review aims to provide recent research regarding the chemical composition, biological activities and safety of honey, which might be attributed to comprehensive utilization of honey. © 2023 Society of Chemical Industry.

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Tualang honey inhibits cell proliferation and promotes apoptosis of human lung adenocarcinoma cells via apoptosis signaling pathway

Cited by 5 publications

References 36 publications

Leptospermum extract (QV0) suppresses pleural mesothelioma tumour growthin vitroandin vivoby mitochondrial dysfunction associated apoptosis

Leptospermum extract (QV0) suppresses pleural mesothelioma tumour growthin vitroandin vivoby mitochondrial dysfunction associated apoptosis

Leptospermum extract (QV0) suppresses pleural mesothelioma tumor growth in vitro and in vivo by mitochondrial dysfunction associated apoptosis

Composition, functional properties and safety of honey: a review

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