TTDA inhibited apoptosis by regulating the p53-Bax/Bcl2 axis in glioma

Bai, Huan-Lan; Kang, Chun‐Min; Sun, Zhenqiang; Li, Xue-Heng; Dai, Xiaoyan; Huang, Ruiying; Zhao, Jingjing; Bei, Yan-Rou; Huang, Xianzhang; Lu, Zhifeng; Wu, Shao-Guo; Lu, Jing-Bo; Ping, Baohong; Wang, Qian; Hu, Yan‐Wei

doi:10.1016/j.expneurol.2020.113380

Cited by 24 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Section: Results and Discussionmentioning

confidence: 99%

“…B-cell lymphoma 2 (BCL2)-associated X (BAX) and caspase 3 are downstream target genes of tumor protein p53, which plays an important role in mitochondrial pathway apoptosis. 51 P53 inhibition in pancreatic cancer cells could enhance MDM2 expression and reduce BAX, caspase 3, caspase 9, and cyclindependent kinase inhibitor 1A (p21). 52 Bai et al found that cytosine-phosphorothioate-guanine oligodeoxynucleotides could induce apoptosis in human bladder cancer cells by enhanced expressions of pro-apoptotic-related factors caspase 3, BAX, and p53, as well as reduced BCL2 expression.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hormesis Effect of Methyl Triclosan on Cell Proliferation and Migration in Human Hepatocyte L02 Cells

Yao

Tang

et al. 2021

ACS Omega

View full text Add to dashboard Cite

Methyl triclosan (mTCS) is a methylated derivative of triclosan (TCS), which is extensively used as an antimicrobial component of various nursing products and disinfectants. Current research studies of mTCS mainly focused on the environmental persistence and bioaccumulation potential. Knowledge regarding the toxicity and carcinogenicity of mTCS is limited until now. In this study, the human hepatocyte L02 cells were used to investigate the cellular effects of mTCS under different concentrations (0.1–60 μM). The hormesis effect was observed where a low dose of mTCS (≤5 μM) exposure stimulated the cell proliferation ability, while high-dose exposure (≥20 μM) inhibited cell proliferation. In the same time, low doses of mTCS (0.5 and 1 μM) induced enhanced anchorage-independent proliferation ability and cell migration ability, indicating a positive effect on malignant transformation in L02 cells. Moreover, reactive oxygen species productions were significantly increased after mTCS exposure (≥1 μM), as compared with the control group. Furthermore, expressions of tumor-related genes, mouse double minute 2 (MDM2), matrix metalloproteinase 9 (MMP9), and proliferating cell nuclear antigen (PCNA), and proto-oncogene MYC (c-Myc), Jun, and FosB were significantly upregulated, while no significant changes were observed on expressions of apoptosis-related and cell cycle-related genes in L02 cells after exposure of low-dose mTCS. In conclusion, these results indicated that a low dose of mTCS had a hormesis effect in L02 cells on cell proliferation and malignant transformation in vitro, which might be mediated through oxidative stress response.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Hormesis Effect of Methyl Triclosan on Cell Proliferation and Migration in Human Hepatocyte L02 Cells

Yao

Tang

et al. 2021

ACS Omega

View full text Add to dashboard Cite

show abstract

“…To further validate whether DBDCT induced apoptosis of HL7702 cells via the Gal-1-dependent pathway, the expressions of apoptosis-associated proteins in HL7702 cells were detected. e expressions of the antiapoptotic protein Bcl-2 and the proapoptotic protein Bax and p53 are crucial determinants of the apoptotic response mediated by many agents [38,39]. Accumulating evidence has shown that a decrease in the ratio of Bax/Bcl-2 inhibits Bax translocation to the mitochondria and then protects cells against apoptotic insults; however, a shift in the balance towards an excess of Bax evokes apoptosis [38,39].…”

Section: Discussionmentioning

confidence: 99%

Galectin‐1‐Dependent Mitochondria Apoptosis Plays an Essential Role in the Potential Protein Targets of DBDCT‐Induced Hepatotoxicity as Revealed by Quantitative Proteomic Analyses

Song

Ren

Liu

et al. 2022

Bioinorganic Chemistry and Applications

View full text Add to dashboard Cite

Di-n-butyl-di-(4-chlorobenzohydroxamato) tin(IV) (DBDCT), a new patent agent, exhibited strong antitumor activity. In some cases, its activity was close to or even higher than cisplatin, a first-line clinical metallic agent. Similar to platinum compounds, it also showed toxicity. However, the effective targets and mechanisms for specific toxicity and biological activity are still unclear. In this study, proteomic analysis revealed that 146 proteins (98 upregulated and 48 downregulated) were differentially identified by label-free LC-MS/MS after DBDCT treatment. Meanwhile, network analysis of these differential proteins suggested that protein Galectin-1 (Gal-1) could regulate the apoptosis process (15 related proteins), which played an essential role in the potential targets of DBDCT-induced hepatotoxicity. Furthermore, it was demonstrated that DBDCT might promote ROS production, activate NF-κB p65, inhibit Ras and p-ERK1/2 expressions, increase the level of Gal-1, subsequently upregulate the expressions of Bax, p53, Fas, and FasL, and downregulate the expression of Bcl-2. As a result of these modulations, caspase cascades were finally activated, which executed apoptosis in HL7702 liver cells. Correspondingly, NAC (inhibitor of ROS), PDTC (inhibitor of NF-κB), EGF (ERK1/2 activator), and OTX008 (inhibitor of Gal-1) were found to reverse and abolish the DBDCT-associated cytotoxicity partially. In conclusion, Gal-1 might be the potential target for toxicity and biological activity. Moreover, the present study will lay the groundwork for future research about di-n-butyl-di-(4-chlorobenzohydroxamato) tin structure optimization and developing it into a new potential anticancer agent.

show abstract

“…The results of GSEA enrichment analysis demonstrate that several pathways and biology functions are positively associated with risk scores such as p53 signaling pathway, cell cycle pathway, DNA replication pathway, DNA post-replication repair pathway. The p53 is a famous tumor suppressed factor which is signi cantly associated with some important roles such as DNA damage and oncogenic signaling, and its dysfunction can promote cancer development and deterioration [39,40]. Deviant cell cycle is the most common variation during tumor onset and development [41].…”

Section: Discussionmentioning

confidence: 99%

Identification of A Glycolysis-Related Seven-lncRNA Signature to Predict Survival in Diffuse Glioma Patients

Huang

Bai

et al. 2022

Preprint

View full text Add to dashboard Cite

Background: Glioma, the most common tumor in brain, seriously threaten human’s healthy. Glycolysis is a crucial hallmark of malignant tumor which can facilitate cancer progression and deterioration. Long noncoding RNAs (LncRNAs) are increasingly implicated in disease progression. Therefore, our study plans to reveal whether glycolysis-related LncRNAs can independently predict prognosis of glioma patients. Methods: Firstly, co-expression network between glycolysis-related genes and LncRNAs is constructed using Pearson correlation. Secondly, univariate Cox regression and the Least Absolute Shrinkage and Selection Operator (LASSO) analysis are used to identify key glycolysis-related LncRNAs. Thirdly, based on multivariate Cox regression, a predictive model is built. At last, the Kaplan–Meier survival analysis, receiver operating characteristic (ROC) and gene set enrichment analysis (GSEA) are performed to assess the effect of risk score among glioma patients. Results: A glycolysis-related LncRNAs risk signature based on nine LncRNAs was identified. The risk score calculation formula is yielded as follows: risk score = (−0.16 × TPM of RP11-294N21.3) + (0.22 × TPM of AC093627.7) + (0.09 × TPM of AC093627.10) + (0.05 × TPM of RP11-359G22.2) +(0.17 × TPM of LINC01272) + (0.05 × TPM of AC092484.1) + (0.01 × TPM of AC026904.1). The risk score is independently associated with prognosis, and each identified LncRNAs is significantly related to the overall survival of patients. Moreover, the results of GSEA analysis indicate that the high-risk score is mainly involved in some vital biologic process.Conclusion: The risk model based on our LncRNAs signature can significantly predict prognosis and may serve as potential therapeutic targets in the future.

show abstract

TTDA inhibited apoptosis by regulating the p53-Bax/Bcl2 axis in glioma

Cited by 24 publications

References 34 publications

Hormesis Effect of Methyl Triclosan on Cell Proliferation and Migration in Human Hepatocyte L02 Cells

Hormesis Effect of Methyl Triclosan on Cell Proliferation and Migration in Human Hepatocyte L02 Cells

Galectin‐1‐Dependent Mitochondria Apoptosis Plays an Essential Role in the Potential Protein Targets of DBDCT‐Induced Hepatotoxicity as Revealed by Quantitative Proteomic Analyses

Identification of A Glycolysis-Related Seven-lncRNA Signature to Predict Survival in Diffuse Glioma Patients

Contact Info

Product

Resources

About