TT‑1, an analog of melittin, triggers apoptosis in human thyroid cancer TT cells via regulating caspase, Bcl‑2 and Bax

Abstract

Abstract. Melittin is a 26 amino acid residue antimicrobial peptide with known antitumor activity. In the present study, a novel peptide TT-1, derived from melittin and contained only 11 amino acids, was designed, and its antitumor effect was investigated. The present study is aimed to elucidate the effects and relative mechanisms of TT-1 on a human thyroid cancer cell line (TT) in vitro and in vivo. Cell viability assays, Annexin V/propidium iodide assays, western blotting and quantitative reverse transcription polymerase chain reaction were performed. Furthermore, a tumor-xenograft model was established to investigate the apoptotic mechanisms of TT-1 on TT cells. The results obtained indicated that TT-1 was able to suppress the proliferation of TT cells and exhibited low cytotoxicity to normal thyroid cells in vitro. The apoptotic rates of TT cells were also increased following TT-1 treatment. Additionally, TT-1 stimulated caspase-3, caspase-9 and Bax, and inhibited B-cell lymphoma 2 mRNA and protein expression. Finally, it was also demonstrated that TT-1 is able to markedly suppress tumor growth in a TT-bearing nude mouse model. In summary, TT-1 may inhibit the proliferation of TT cells by inducing apoptosis in vitro and in vivo, indicating that TT-1 may be a potential candidate for the treatment of thyroid cancer.

Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect

“…1). The apoptotic rates of human thyroid cancer cell line (TT) cells were increased following melittin treatment, melittin causes increased Caspase3, Caspase9, Bax, and inhibited B-cell lymphoma 2 genes, and protein expression (Wan 2018). Melittin decreased the invasion rate of MCF-7 human breast cancer cell line by down-regulating CD147, and MMP-9 by inhibiting cyclophilin A expression so the results provide evidence for melittin in anticancer therapy, and mechanisms (Wang et al 2017).…”

Delivery of Melittin Loaded Niosomes for Breast Cancer Treatment: an in-vitro and in-vivo Evaluation of Anti-cancer Effect

“…11), the administration of Melittin loaded niosome shows the synergic and higher up-regulating effects in (Bax, Caspase3 and Caspase9 genes) and downregulating (Bcl2, MMP2, and MMP9 genes) in both cell lines.The apoptotic rates of human thyroid cancer cell line (TT) cells were increased following Melittin treatment, Melittin causes increased Caspase3, Caspase9, Bax, and inhibited B-cell lymphoma 2 genes and protein expression[59].Melittin decreased the invasion rate of MCF-7 human breast cancer cell line by down-regulating CD147 and MMP-9 by inhibiting cyclophilin A expression so the results provide an evidence for Melittin in anticancer therapy and mechanisms[60].Honey bee venom contain Melittin and chrysin are effective for destroying chemoresistant ovarian cancer cells through upregulation of Caspase3 and Caspase9 and down-regulation of Bcl2 genes expression[61].…”

Anti-Proliferation Activity of a Decapeptide from Perinereies aibuhitensis toward Human Lung Cancer H1299 Cells

“…Huang [ 22 ] reported that SIO induced the death of prostate cancer cells (DU-145, PC-3, and LNCaP) and showed an increase in the Bax/Bcl-2 ratio. Furthermore, Wan et al [ 28 ] found that an oligopeptide (KIKAVLKVLTT) derived from melittin induced the death of thyroid cancer TT cells via upregulation of Bax and down-regulation of Bcl-2. Our results also indicated that PAP induced apoptosis of H1299 cells by up-regulating the Bax/Bcl-2 ratio, which was consistent with previous studies.…”