Tsuneonella litorea sp. nov., a novel carotenoid-producing bacterium isolated from coastal sediment

Abstract

A Gram-stain-negative, aerobic, non-motile and pleomorphic bacterium designated as YG55T was isolated from a coastal sediment sample. Growth was found to occur at 10–37 °C (optimum, 28 °C), at pH 6–9 (optimum, pH 8) and in 0–6 % NaCl (optimum, 1 %). The results of 16S rRNA gene-based analysis showed that strain YG55T was related to the members of the genus Tsuneonella and shared the highest identity of 99.4 % with Tsuneonella dongtanensis GDMCC 1.2307T, followed by Tsuneonella troitsensis JCM 17037T (98.4 %). The phylogenomic results indicated that strain YG55T formed an independent branch distinct from the reference type strains. The 22.7 and 21.8 % digital DNA–DNA hybridization (dDDH) values and 83.0 and 81.8 % average nucleotide identity (ANI) values between strain YG55T and the two relatives were below the species definition thresholds of 70 % (dDDH) and 95–96 % (ANI), indicating that the strain represents a novel genospecies. The results of chemotaxonomic characterization indicated that the major cellular fatty acids of strain YG55T were summed feature 8 (C18 : 1 ω6c and/or C18 : 1 ω7c), C14 : 0 2OH and C16 : 0; the main polar lipids comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine and sphingoglycolipid; the respiratory quinone was ubiquinone-10. The genomic size and DNA G+C contents were 3.03 Mbp and 66.98 %. The strain contained carotenoid biosynthesis genes and could produce carotenoids. Based on its genotypic and phenotypic characteristics, strain YG55T is concluded to represent a novel species of the genus Tsuneonella , for which the name Tsuneonella litorea sp. nov. is proposed. The type strain is YG55T (=GDMCC 1.2590 T=KCTC 82812T).

Isolation and Cultivation of Carotenoid-Producing Strains from Tidal Flat Sediment and Proposal of Croceibacterium aestuarii sp. nov., a Novel Carotenoid-Producing Species in the Family Erythrobacteraceae

“…Currently, the family Erythrobacteraceae accommodates 20 genera (https://lpsn.dsmz.de/family/erythrobacteraceae, accessed on 27 December 2023) [14]. Ecologically, the family Erythrobacteraceae strains are commonly isolated from multiple marine environments, including seawater [15,16], sediments [17,18], as well as marine fauna and flora [19][20][21]. The genus Croceibacterium, belonging to the family Erythrobacteraceae, was proposed by Liu et al [22], describing Croceibacterium ferulae as the type species and reclassifying Porphyrobacter mercurialis as C. mercuriale.…”