Tsukamurella tyrosinosolvens sp. nov.

Yassin, A. F.; Rainey, Fred A.; Burghardt, Jutta; Brzezinka, H.; Schmitt, Stephan; Seifert, Paul S.; Zimmermann, Ortrud; Mauch, H.; Gierth, D.; Lux, I.; Schaal, K. P.

doi:10.1099/00207713-47-3-607

Cited by 73 publications

(44 citation statements)

References 6 publications

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“…With few discrepancies, our study of 15 strains of Tsukamurella generally showed good agreement between our phenotypic data and those reported by Yassin et al for T. paurometabola, T. inchonensis, T. pulmonis, and T. tyrosinosolvens (34)(35)(36). Testing for sugar utilization with the API 50 CH system showed excellent correlation with published data based on tests with conventional biochemicals.…”

Section: G Aurantiaca Was Renamed Rhodococcus Aurantiacus By Tsupporting

confidence: 79%

“…Testing for sugar utilization with the API 50 CH system showed excellent correlation with published data based on tests with conventional biochemicals. However, with degradation agars, we found inconsistencies among different studies and with our results (2,24,(34)(35)(36). Moreover, in our study we found discrepancies between different commercial systems.…”

Section: G Aurantiaca Was Renamed Rhodococcus Aurantiacus By Tcontrasting

confidence: 56%

“…Tsukamurella wratislaviensis was isolated from soil (10). Strains of Tsukamurella inchonensis, Tsukamurella pulmonis, and Tsukamurella tyrosinosolvens have been isolated only from human specimens, and all were associated with clinical disease (34)(35)(36). We have encountered an unusual isolate in multiple cultures of blood from a 5-year-old girl with acute myelogenous leukemia who presented with sepsis.…”

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confidence: 99%

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Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Kattar

Cookson²,

Carlson³

et al. 2001

J Clin Microbiol

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We have isolated a gram-positive, weakly acid-alcohol-fast, irregular rod-shaped bacterium from cultures of blood from a 5-year-old girl with acute myelogenous leukemia. This isolate was compared with 14 other strains including reference strains of Tsukamurella species by a polyphasic approach based on physiological and biochemical properties, whole-cell short-chain fatty acid and mycolic acid analyses, DNA-DNA hybridization, and sequencing of the 16S rRNA gene. This isolate represents a new taxon within the genus Tsukamurella for which we propose the name Tsukamurella strandjordae sp. nov. Our study also revealed that Tsukamurella paurometabola ATCC 25938 represents a misnamed Tsukamurella inchonensis isolate and confirms that Tsukamurella wratislaviensis belongs to the genus Rhodococcus.Tsukamurellae are members of the mycolic acid-containing aerobic actinomycetes. The genus was created in 1988 to accommodate a group of chemically unique organisms characterized by a series of very long chain (68 to 76 carbons) highly unsaturated (two to six double bonds) mycolic acids, in addition to possessing meso-diaminopimelic acid and arabinogalactan, common to the genus Corynebacterium (6). The type species, Tsukamurella paurometabola, described by Steinhaus as Corynebacterium paurometabolum in 1941, was originally isolated from the mycetomes and ovaries of bed bugs (27). The first human isolate of Tsukamurella was reported in 1971 as Gordona aurantiaca (33). Four additional species were proposed in the 1990s. Tsukamurella wratislaviensis was isolated from soil (10). Strains of Tsukamurella inchonensis, Tsukamurella pulmonis, and Tsukamurella tyrosinosolvens have been isolated only from human specimens, and all were associated with clinical disease (34-36). We have encountered an unusual isolate in multiple cultures of blood from a 5-year-old girl with acute myelogenous leukemia who presented with sepsis. On the basis of physiological and biochemical characteristics, analysis of cell components, DNA-DNA hybridizations, and the 16S rRNA gene sequence, we propose that this strain represents a new taxon within the genus Tsukamurella to which we assign the name Tsukamurella strandjordae sp. nov. Our study compared this isolate to reference and clinical strains of the other Tsukamurella species and found that T. paurometabola ATCC 25938 is a misnamed T. inchonensis isolate. MATERIAL AND METHODSStrains. The study included type strains of all proposed Tsukamurella species. T. paurometabola ATCC 8368 and T. wratislaviensis ATCC 51786 were purchased from the American Type Culture Collection (ATCC). A. F. Yassin generously provided the type strains T. pulmonis ATCC 700081 and T. inchonesis ATCC 700082. Type strain T. tyrosinosolvens DSMZ 44234 was purchased from the Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH (DSMZ; Braunschweig, Germany). Other purchased reference strains included strains T. paurometabola ATCC 25938 and T. tyrosinosolvens DSMZ 44316. The strain of T. strandjordae, the subject of this study, was is...

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Section: G Aurantiaca Was Renamed Rhodococcus Aurantiacus By Tsupporting

confidence: 79%

Section: G Aurantiaca Was Renamed Rhodococcus Aurantiacus By Tcontrasting

confidence: 56%

mentioning

confidence: 99%

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Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Kattar

Cookson²,

Carlson³

et al. 2001

J Clin Microbiol

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“…Asymmetrical daughter cells were generated from the fragmentation process of the transverse septa at one pole of each parental cell during growth (Fig. 2b, d), which was also in congruence with observations reported in previous studies (Yassin et al, 1996(Yassin et al, , 1997Teng et al, 2015).…”

supporting

confidence: 81%

Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra)

Tang

Teng

Cheung

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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“…Specifically, upon review of a Gram stain of the blood isolate, the differential was expanded to include mycobacterial pathogens and other aerobic actinomycoses, as well as Tsukamurella spp., in part due to the clinical picture and overlapping laboratory findings (11). Three days later, the patient developed acute renal insufficiency, due to the TMP-SMZ, and was rehospitalized for further management of his renal disease.…”

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confidence: 99%

Misidentification of Mycobacterium peregrinum , the Causal Organism of a Case of Bacteremia and Automatic Implantable Cardioverter Defibrillator-Associated Infection, Due to Its Unusual Acid-Fast Staining Characteristics

et al. 2005

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We report an unusual case of Mycobacterium peregrinum bacteremia and infection of an automatic implantable cardioverter defibrillator that was originally misidentified as a Nocardia sp. due, in part, to its partially acid-fast staining characteristic, morphology, and odor. The misdiagnosis had a direct effect on patient care, though the patient was subsequently successfully treated. CASE REPORTA 74-year-old white male was admitted with a past history of ischemic cardiomyopathy, coronary artery disease, hypertension, and a recent diagnosis of nonsustained ventricular tachycardia requiring placement of an automatic implantable cardioverter defibrillator (AICD) 6 weeks earlier. He presented to our institution with persistent erythema and a history of a pustule around the AICD insertion site on his left chest wall. The patient stated he immediately noted erythema around the insertion site after AICD placement and was prescribed a 10-day course of cephalexin at a 3-week follow-up with his cardiologist. Subsequently, he noted a pustule along the suture line and was admitted for further evaluation and management. On physical examination he was nontoxic in appearance and afebrile, his vital signs were stable, and his physical examination was within normal limits except for the left chest area. The left chest wall was erythematous in an area of 4 to 5 cm where the AICD site had been inserted subcutaneously; there was a deep purple discoloration along the incision line, and the area was warm to touch and minimally tender to palpation. There was no obvious pustule or purulent discharge along the incision, and the surrounding area was not fluctuant. Laboratory data were unremarkable except for a creatinine level of 1.7 mg/dl, which was his baseline. Blood cultures were drawn on admission, and empirical vancomycin was begun. On the day of admission he underwent extraction of the AICD and the leads in the electrophysiology laboratory. Deep-pocket tissue and superficial wound specimens were sent for routine bacterial, fungal, and acid-fast bacillus cultures.The described wound specimens and two sets of blood cultures were submitted to the microbiology laboratory for bacterial culture. Direct Gram-stained smears of the wound cultures demonstrated rare to few polymorphonuclear leukocytes with no organisms seen. After 4 days of incubation, growth was detected in both blood cultures and in the thioglycolate broth media of both wound cultures.On the fifth hospital day, Gram-stained smears of the wound broth cultures were reported as having gram-positive rods. One day later, growth was detected on the sheep blood and chocolate agar plates, and no growth was detected on the MacConkey agar plate. Colonies were tan, flat, and dry and upon further incubation became wrinkled in appearance and exhibited a musty odor. The Vitek Identification System (bioMérieux, Hazelwood, MO) was unable to identify the isolate. The isolate was subcultured to Columbia agar for identification using the API CORYNE system (bioMérieux, Hazelwood, MO), but it t...

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Tsukamurella tyrosinosolvens sp. nov.

Cited by 73 publications

References 6 publications

Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Tsukamurella strandjordae sp. nov., a Proposed New Species Causing Sepsis

Tsukamurella serpentis sp. nov., isolated from the oral cavity of Chinese cobras (Naja atra)

Misidentification of Mycobacterium peregrinum , the Causal Organism of a Case of Bacteremia and Automatic Implantable Cardioverter Defibrillator-Associated Infection, Due to Its Unusual Acid-Fast Staining Characteristics

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