tsRNA Landscape and Potential Function Network in Subcutaneous and Visceral Pig Adipose Tissue

Abstract

Noncoding RNAs (ncRNAs) called tsRNAs (tRNA-derived short RNAs) have the ability to regulate gene expression. The information on tsRNAs in fat tissue is, however, limited. By sequencing, identifying, and analyzing tsRNAs using pigs as animal models, this research reports for the first time the characteristics of tsRNAs in subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT). A total of 474 tsRNAs, 20 and 21 of which were particularly expressed in VAT and SAT, respectively, were found in WAT. According to the analysis of the tsRNA/miRNA/mRNA co-expression network, the tsRNAs with differential expression were primarily engaged in the endocrine and immune systems, which fall under the classification of organic systems, as well as the global and overview maps and lipid metropolis, which fall under the category of metabolism. This research also discovered a connection between the activity of the host tRNA engaged in translation and the production of tsRNAs. This research also discovered that tRF-Gly-GCC-037/tRF-Gly-GCC-042/tRF-Gly-CCC-016 and miR-218a/miR281b may be involved in the regulation of fatty acid metabolism in adipose tissue through SCD based on the tsRNA/miRNA/mRNA/fatty acid network. In conclusion, our findings enrich the understanding of ncRNAs in WAT metabolism and health regulation, as well as reveal the differences between SAT and VAT at the level of tsRNAs.

Longitudinal observation of tRNA‐derived fragments profiles in gestational diabetes mellitus and its diagnostic value

“…34 Zeng et al 35 indicated that the triglyceride-glucose index is positively correlated with the occurrence of GDM, while Grieger et al 36 identified triglyceride concentration as an independent risk factor for GDM. Wang et al 37 investigated the role of various tRFs and microRNAs in regulating fatty acid metabolism within mammalian subcutaneous and visceral adipose tissues, with a particular focus on stearoyl-CoA desaturase (SCD). Despite these insights, further research is required to fully understand how tsRNAs influence the development of GDM through their involvement in lipid metabolism.…”

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