TSPY4 is a novel sperm-specific biomarker of semen exposure in human cervicovaginal fluids; potential use in HIV prevention and contraception studies

Jacot, Terry A.; Zalenskaya, Irina A.; Mauck, Christine; Archer, David F.; Doncel, Gustavo F.

doi:10.1016/j.contraception.2012.11.022

Cited by 20 publications

(27 citation statements)

References 37 publications

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“…Multiplex PCR is beneficial because multiple genes may be assessed using a single aliquot of sample [32]. …”

Section: Discussionmentioning

confidence: 99%

“…We sent the supernatant to the Centers for Disease Control and Prevention for PSA measurements, as previously described [31]. The pellet was resuspended in 700 μL PBS, and half was used for SRY qPCR (labeled “YcDNA”) analysis, and the other half was used for SRY-TSPY4 multiplex PCR and TSPY4 qPCR analysis, both performed as previously described [32,33]. …”

Section: Methodsmentioning

confidence: 99%

“…We used both qPCR and multiplex PCR to assess both Y chromosome biomarkers to measure semen exposure. Although both assays detect the SRY region of the Y chromosome, we use the terms SRY for the multiplex system and YcDNA for the qPCR system, to maintain consistency with previously published data [26,32,33]. …”

Section: Methodsmentioning

confidence: 99%

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Assessment of the vaginal residence time of biomarkers of semen exposure

et al. 2016

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Objective The primary objective of this pilot study is to determine and compare the residence time in the vagina of biomarkers of semen exposure for up to 15 days post exposure. The biomarkers are prostate-specific antigen (PSA), Y chromosome DNA, the sex determining region of the Y chromosome (SRY) and testis-specific protein Y-encoded 4 (TSPY4). The secondary objectives are to determine if biomarker concentrations differed between intercourse and inoculation groups, to establish whether the sampling frequency post exposure affected biomarker concentrations and decay profile and to determine if biomarker concentrations in vaginal swabs obtained by the participant at home were similar to swabs obtained by the nurse in the clinic. Study design We randomized healthy women to unprotected intercourse (n=17) versus vaginal inoculation with the male partner’s semen in the clinic (n=16). Women were then further randomized to have vaginal swabs obtained at either 7 or 4 time points after semen exposure, up to 15 days post exposure, either obtained at home by the participant or in the clinic by the research nurse. Results PSA and SRY were markers of recent semen exposure. TSPY4 was detectable in approximately 50% of participants at 15 days post exposure. Unprotected intercourse resulted in significantly higher concentrations of select biomarkers. Sampling frequency and home versus clinic sampling had no significant effect on biomarker concentrations. Conclusions Objective biomarkers of recent or distant semen exposure may have great utility for verifying protocol compliance in a variety of clinical trials.

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“…Multiplex PCR is beneficial because multiple genes may be assessed using a single aliquot of sample [32]. …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Assessment of the vaginal residence time of biomarkers of semen exposure

et al. 2016

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“…Ccdc89 is a potential regulator of neurogenesis, interacting with the orange domain of the hairy-related transcription factor (HRT/Hey1) in Xenopus and mouse [106]. The nuclear protein Tspy4 is a member of the Tspy (testis-specific protein Y-encoded) family and, as such, is a sperm-specific biomarker in human cervico-vaginal fluids [107]. However the recent discovery that Tspy1 is found together with the multi-domain adapter protein CASK in neuronal axon fibers in the brain [108] could be the starting point to further investigate the role of Tspy4 in the development of the brain.…”

Section: Resultsmentioning

confidence: 99%

Transcriptional Analysis of Apoptotic Cerebellar Granule Neurons Following Rescue by Gastric Inhibitory Polypeptide

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Calissano

et al. 2014

IJMS

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Apoptosis triggered by exogenous or endogenous stimuli is a crucial phenomenon to determine the fate of neurons, both in physiological and in pathological conditions. Our previous study established that gastric inhibitory polypeptide (Gip) is a neurotrophic factor capable of preventing apoptosis of cerebellar granule neurons (CGNs), during its pre-commitment phase. In the present study, we conducted whole-genome expression profiling to obtain a comprehensive view of the transcriptional program underlying the rescue effect of Gip in CGNs. By using DNA microarray technology, we identified 65 genes, we named survival related genes, whose expression is significantly de-regulated following Gip treatment. The expression levels of six transcripts were confirmed by real-time quantitative polymerase chain reaction. The proteins encoded by the survival related genes are functionally grouped in the following categories: signal transduction, transcription, cell cycle, chromatin remodeling, cell death, antioxidant activity, ubiquitination, metabolism and cytoskeletal organization. Our data outline that Gip supports CGNs rescue via a molecular framework, orchestrated by a wide spectrum of gene actors, which propagate survival signals and support neuronal viability.

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“…The multiplex PCR also amplifies 2 Y chromosomal genes, the sex determining region of the Y chromosome (SRY) and testis-specific Y encoded 4 (TSPY4), from vaginal swab DNA if semen exposure occurred. 10 If the multiplex PCR amplified one or both of these genes, it was concluded that semen exposure occurred within approximately 7 days of applicator insertion.…”

Section: Methodsmentioning

confidence: 99%